OBJECTIVE

2

To establish the fingerprint of Pulan liyan mixture， and content determination method of 8 components such as chlorogenic acid so as to evaluate the quality of Pulan liyan mixture.

METHODS

2

Using baicalin as reference peak， high-performance liquid chromatography （HPLC） fingerprints of 11 batches of Pulan liyan mixture were established by the

Chromatographic Fingerprint Similarity Evaluation System of TCM

（2012 edition） for similarity evaluation and identifying the common peaks. Cluster analysis， principal component analysis and orthogonal partial least squares-discriminant analysis of those samples were performed with SPSS 20.0 software and SIMCA 14.1 software. The variable importance projection （VIP） value greater than 1 was used as the index to screen the markers affecting the quality of Pulan liyan mixture； the contents of 8 components such as chlorogenic acid were determined by the same HPLC method.

RESULTS

2

Totally 20 common peaks in the fingerprints of 11 batches of Pulan liyan mixture was established， and the similarities were 0.973-0.994. The 8 peaks were identified， which were chlorogenic acid， caffeic acid， loganin， cichoric acid， styroside B， baicalin， melaleucalycin A-7-

O-β

-D-glucuronic acid and wogonin. The results of cluster analysis showed that the 11 batches of samples were divided into two categories， S3， S10 and S11 were grouped into one category， and the other batches of samples were grouped into one category. The results of principal component analysis showed that the cumulative variance contribution rate of the first four principal components was 95.546%， and the classification results were consistent with the cluster analysis results. The results of orthogonal partial least squares-discriminant analysis showed that 15， 14 （baicalin）， 10， 18 （wogonin）， 11， 17 （laminarin A-7-

O

-

β

-D-glucuronic acid）， 4， 20， 16， 12， 7 and 19 peaks had VIP values greater than 1. The contents of chlorogenic acid， caffeic acid， loganin， cichoric acid， styroside B， baicalin， melaleucalycin A-7-

O-β

-D-glucuronic acid and wogonin were 0.287-1.021， 0.163-0.485， 0.735-3.641， 0.587-4.012， 1.920-9.063， 37.443-115.974， 3.623-13.942 and 7.135-18.736 mg/g， respectively.

CONCLUSIONS

2

The established HPLC fingerprint and the determination method of 8 components such as chlorogenic acid could be used for the quality evaluation of Pulan liyan mixture. The 12 components， such as baicalin， wogonoside， melaleucalycin A-7-

O

-

β

-D-glucuronic acid， may be the markers affecting the quality of Pulan liyan mixture.