Effects of <italic style="font-style: italic">Citrus medica</italic> before and after being steamed on dryness indexes and blood metabolism in rats

NIE Qinxin; ZHANG Qiuxia; WANG Jinyu; WU Peijun; HE Yali; LIN Hongbin

doi:10.6039/j.issn.1001-0408.2022.23.21

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Effects of Citrus medica before and after being steamed on dryness indexes and blood metabolism in rats

更新时间：2023-02-22

- Effects of Citrus medica before and after being steamed on dryness indexes and blood metabolism in rats
- ZHONGGUO YAOFANG Vol. 33, Issue 23, Pages: 2926-2930(2022)
- 作者机构：
  
  1.广州中医药大学中药学院，广州　510006
  2.广州实验室药物与疫苗研究部，广州　510005
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2022.23.21
  CLC： R965
- Published：15 December 2022，
  
  Received：08 June 2022，
  
  Revised：02 September 2022，
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聶沁馨,张秋霞,汪金玉等.佛手蒸制前后对大鼠燥性指标及血液代谢的影响 ^Δ[J].中国药房,2022,33(23):2926-2930.

NIE Qinxin,ZHANG Qiuxia,WANG Jinyu,et al.Effects of Citrus medica before and after being steamed on dryness indexes and blood metabolism in rats[J].ZHONGGUO YAOFANG,2022,33(23):2926-2930.
聶沁馨,张秋霞,汪金玉等.佛手蒸制前后对大鼠燥性指标及血液代谢的影响 ^Δ[J].中国药房,2022,33(23):2926-2930. DOI： 10.6039/j.issn.1001-0408.2022.23.21.

NIE Qinxin,ZHANG Qiuxia,WANG Jinyu,et al.Effects of Citrus medica before and after being steamed on dryness indexes and blood metabolism in rats[J].ZHONGGUO YAOFANG,2022,33(23):2926-2930. DOI： 10.6039/j.issn.1001-0408.2022.23.21.

摘要

目的

探究佛手蒸制前后对大鼠燥性相关指标和血液代谢的影响。

方法

将24只SD大鼠随机分为空白组、生佛手组（2.4 g/kg）、制佛手组（2.4 g/kg）、枳壳组（阳性对照，1.2 g/kg），每组6只。连续给药5周，动态观察大鼠体质量、摄食量、饮水量、尿液量，检测大鼠血清中肾水通道蛋白3（AQP3）含量。采用液相色谱-质谱联用技术对大鼠血清样本进行代谢轮廓分析，通过主成分分析（PCA）、正交偏最小二乘法-判别分析（OPLS-DA）等方法筛选差异代谢物，并将筛选后的差异代谢物映射到KEGG数据库进行通路分析。

结果

各组大鼠体质量均平缓上升；制佛手组大鼠的摄食量、饮水量均具有高于生佛手组和枳壳组的趋势，但差异无统计学意义（

＞0.05）。给药第21天，与枳壳组比较，制佛手组大鼠尿液量显著升高（

＜0.05），血清AQP3含量显著降低（

＜0.05），且均接近空白组。代谢组学研究发现，生佛手主要影响机体的脂代谢，制佛手主要影响机体的氨基酸代谢；生佛手组和制佛手组之间的差异代谢物涉及半胱氨酸和蛋氨酸代谢，甘氨酸、丝氨酸和苏氨酸代谢，牛磺酸和次牛磺酸代谢等通路。

结论

制佛手可能通过影响环磷酸腺苷-蛋白激酶A通路缓和生佛手的燥性，血清AQP3含量可作为评价佛手炮制前后燥性变化的指标；生佛手和制佛手对大鼠血清内源性代谢物的影响存在较大差异，尤其在脂质代谢方面。

Abstract

OBJECTIVE

To explore the effects of

Citrus medica

before and after being steamed on blood metabolism and dryness indexes in rats.

METHODS

Twenty-four SD rats were randomly divided into blank group，

C. medica

group （2.4 g/kg）， processed

C. medica

group （2.4 g/kg），

Citrus aurantium

group （positive control， 1.2 g/kg）， with 6 rats in each group. After 5 weeks of continuous administration， the body weight， food intake， water intake and urine volume were observed dynamically. The content of aquaporin 3 （AQP3） in serum was detected. The metabolic profile of rat serum samples was analyzed by liquid chromatography-mass spectrometry. Principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis （OPLS-DA） were used to screen differential metabolites， and screened differential metabolites were mapped to the KEGG database for pathway analysis.

RESULTS

The body weight of rats in each group increased slowly； the food intake and water intake of processed

C. medica

group tended to be higher than those in

C. medica

group and

C. aurantium

group， but there was no significant difference among those groups （

＞0.05）. On the 21st day of administration， compared with

C. aurantium

group， the urine volume of processed

C. medica

group was significantly increased （

＜0.05）， the serum AQP3 content of processed

C. medica

group was significantly decreased （

＜0.05）， and were close to those of blank group. According to the metabonomic study，

C. medica

mainly affected the lipids metabolism， and processed

C. medica

mainly affected the amino acid metabolism. The differential metabolites pathway between

C. medica

and processed

C. medica

involved the metabolism of cysteine and methionine， metabolism of glycine， serine and threonine， and metabolism of taurine and hypotaurine.

CONCLUSIONS

Processed

C. medica

might relieve the dryness of

C. medica

by affecting adenosine phosphate-protein kinase A. The content of AQP3 in serum can be used as the evaluation index for the dryness of

C. medica

before and after processing. The effects of

C. medica

and processed

C. medica

on endogenous metabolites in rat serum are quite different， especially in the aspect of lipid metabolism.

关键词

佛手蒸制燥性差异代谢物代谢组学

Keywords

steamingdrynessdifferential metabolitesmetabonomics

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Related Institution

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Engineering Research Center for the Development and Application of Ethnic Medicine and TCM （Ministry of Education）， Guizhou Medical University

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Effects of Citrus medica before and after being steamed on dryness indexes and blood metabolism in rats

DOI：10.6039/j.issn.1001-0408.2022.23.21

摘要

Abstract

关键词

Keywords

references