Comparison of protein binding rate of main effective components of Houpu paiqi mixture in animal plasma of different species

GUO Mingxin; FAN Wentao; WU Xia; SHEN Ying; HU Zhiqiang

doi:10.6039/j.issn.1001-0408.2022.24.09

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Comparison of protein binding rate of main effective components of Houpu paiqi mixture in animal plasma of different species

更新时间：2023-02-22

- Comparison of protein binding rate of main effective components of Houpu paiqi mixture in animal plasma of different species
- ZHONGGUO YAOFANG Vol. 33, Issue 24, Pages: 2990-2994(2022)
- 作者机构：
  
  1.江苏大学附属宜兴医院药学部，江苏宜兴　214200
  2.广东药科大学新药研发中心，广州　510006
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2022.24.09
  CLC： R969.1;R927.2
- Published：30 December 2022，
  
  Received：04 June 2022，
  
  Revised：03 November 2022，
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郭明鑫,范文韬,吴霞等.厚朴排气合剂主要药效成分在不同种属动物血浆中蛋白结合率的比较 ^Δ[J].中国药房,2022,33(24):2990-2994.

GUO Mingxin,FAN Wentao,WU Xia,et al.Comparison of protein binding rate of main effective components of Houpu paiqi mixture in animal plasma of different species[J].ZHONGGUO YAOFANG,2022,33(24):2990-2994.
郭明鑫,范文韬,吴霞等.厚朴排气合剂主要药效成分在不同种属动物血浆中蛋白结合率的比较 ^Δ[J].中国药房,2022,33(24):2990-2994. DOI： 10.6039/j.issn.1001-0408.2022.24.09.

GUO Mingxin,FAN Wentao,WU Xia,et al.Comparison of protein binding rate of main effective components of Houpu paiqi mixture in animal plasma of different species[J].ZHONGGUO YAOFANG,2022,33(24):2990-2994. DOI： 10.6039/j.issn.1001-0408.2022.24.09.

摘要

目的

测定厚朴排气合剂主要药效成分厚朴酚、和厚朴酚在不同种属动物血浆中的蛋白结合率并比较种属差异。

方法

采用平衡透析法，将牛、兔、大鼠血浆（即透析内液）置于4.5、9.0、13.5 μg/mL（按厚朴排气合剂饮片质量计）的含药透析液中平衡透析24 h；采用超高效液相色谱-串联三重四极杆质谱技术检测透析内、外液中厚朴酚、和厚朴酚的质量浓度，计算血浆蛋白结合率并进行比较。

结果

在4.5、9.0、13.5 μg/mL质量浓度下，厚朴酚在牛血浆中的蛋白结合率分别为（68.13±4.52）%、（74.34±1.12）%、（86.22±0.50）%，在兔血浆中的蛋白结合率分别为（59.55±4.62）%、（72.81±4.56）%、（86.40±1.91）%，在大鼠血浆中的蛋白结合率分别为（56.63±2.87）%、（77.81±1.83）%、（83.18±0.65）%；和厚朴酚在牛血浆中的蛋白结合率分别为（34.82±1.67）%、（40.29±3.28）%、（63.57±0.59）%，在兔血浆中的蛋白结合率分别为（34.25±5.62）%、（62.12±7.36）%、（80.86±4.01）%，在大鼠血浆中的蛋白结合率分别为（37.06±3.28）%、（52.61±1.69）%、（79.83±7.38）%，组间比较差异大多具有统计学意义（

＜0.05或

＜0.01）。

结论

厚朴排气合剂主要药效成分厚朴酚、和厚朴酚的血浆蛋白结合率存在明显的种属差异，且有一定的浓度依赖趋势。

Abstract

OBJECTIVE

To determine the protein binding rates of main effective components in Houpu paiqi mixture as magnolol and honokiol in animal plasma of different species， and compare the differences among species.

METHODS

The plasma of bovine， rabbit and rat （internal dialysate） was placed in 4.5， 9.0 and 13.5 μg/mL （according to the weight of the decoction pieces of Houpu paiqi mixture） dialysate containing drug for 24 h by equilibrium dialysate method. The concentrations of magnolol and honokiol in dialysis internal and external fluids were determined by ultra-performance liquid chromatography-tandem triple quadrupole mass spectrometry， and the plasma protein binding rates were calculated and compared.

RESULTS

At the concentrations of 4.5， 9.0 and 13.5 μg/mL， the protein binding rates of magnolol were （68.13±4.52）%，（74.34±1.12）% and （86.22±0.50）% in bovine plasma，（59.55±4.62） %，（72.81±4.56）% and （86.40±1.91）% in rabbit plasma， and （56.63±2.87）%，（77.81±1.83）% and （83.18±0.65）% in rat plasma， respectively. The protein binding rates of honokiol were （34.82±1.67）%，（40.29±3.28）% and （63.57±0.59）% in bovine plasma，（34.25±5.62）%，（62.12±7.36）% and （80.86±4.01）% in rabbit plasma， and （37.06±3.28）%，（52.61±1.69）% and （79.83±7.38）% in rat plasma， respectively. There was generally statistical significance among those groups （

＜0.05 or

＜0.01）.

CONCLUSIONS

There are significant species differences in plasma protein binding rates of magnolol and honokiol， the main effective components of Houpu paiqi mixture， in a certain concentration-dependent trend.

关键词

厚朴排气合剂厚朴酚和厚朴酚血浆蛋白结合率平衡透析法牛血浆兔血浆大鼠血浆

Keywords

magnololhonokiolplasma protein binding rateequilibrium dialysisbovine plasmarabbit plasmarat plasma

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