Preliminary study on the effect of Pinggan huoxue powder on cerebral ischemia-reperfusion injury in rats and its mechanism

SONG Faping; ZHANG Jie; TANG Yong; LIU Zongmei; WANG Xiaoling

doi:10.6039/j.issn.1001-0408.2022.24.11

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Preliminary study on the effect of Pinggan huoxue powder on cerebral ischemia-reperfusion injury in rats and its mechanism

更新时间：2023-02-22

- Preliminary study on the effect of Pinggan huoxue powder on cerebral ischemia-reperfusion injury in rats and its mechanism
- ZHONGGUO YAOFANG Vol. 33, Issue 24, Pages: 2999-3004(2022)
- 作者机构：
  
  1.陕西中医药大学第一临床医学院，陕西咸阳　712000
  2.安康市中医医院脑病科，陕西安康　725000
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2022.24.11
  CLC： R932;R285
- Published：30 December 2022，
  
  Received：19 July 2022，
  
  Revised：25 November 2022，
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宋发萍,张洁,唐勇等.平肝活血散对大鼠脑缺血再灌注损伤的影响及作用机制初探 ^Δ[J].中国药房,2022,33(24):2999-3004.

SONG Faping,ZHANG Jie,TANG Yong,et al.Preliminary study on the effect of Pinggan huoxue powder on cerebral ischemia-reperfusion injury in rats and its mechanism[J].ZHONGGUO YAOFANG,2022,33(24):2999-3004.
宋发萍,张洁,唐勇等.平肝活血散对大鼠脑缺血再灌注损伤的影响及作用机制初探 ^Δ[J].中国药房,2022,33(24):2999-3004. DOI： 10.6039/j.issn.1001-0408.2022.24.11.

SONG Faping,ZHANG Jie,TANG Yong,et al.Preliminary study on the effect of Pinggan huoxue powder on cerebral ischemia-reperfusion injury in rats and its mechanism[J].ZHONGGUO YAOFANG,2022,33(24):2999-3004. DOI： 10.6039/j.issn.1001-0408.2022.24.11.

摘要

目的

探讨平肝活血散对大鼠脑缺血再灌注损伤（CIRI）的影响及作用机制。

方法

采用线栓法制备CIRI模型。将大鼠分为假手术组、模型组、阳性对照组（通心络胶囊0.325 g/kg）和平肝活血散低、中、高剂量组（3.02、6.04、12.08 g/kg），每组12只。各组大鼠灌胃等量水或对应药液，每日1次，连续14 d。干预第1、7、14天对大鼠神经功能进行Longa评分。末次给药后，测定大鼠脑梗死体积百分比，观察神经元损伤（神经元密度）和凋亡情况，检测脑缺血皮层中p53、B淋巴细胞瘤2（Bcl-2）、Bcl-2相关X蛋白（Bax）、胱天蛋白酶3（Caspase-3）、活化Caspase-3（Cleaved Caspase-3）蛋白表达水平。

结果

与假手术组比较，模型组大鼠干预第1、7、14天的Longa评分、脑梗死体积百分比均显著升高（

＜0.05）；脑缺血皮层细胞排列紊乱，尼氏小体缺失、空泡，出现大量细胞凋亡；病灶侧神经元密度、Bcl-2蛋白表达均显著降低（

＜0.05），脑缺血皮层中p53、Bax、Caspase-3、Cleaved Caspase-3蛋白表达均显著升高（

＜0.05或

＜0.01）。与模型组比较，平肝活血散高剂量组大鼠脑缺血皮层中Bcl-2表达均显著升高（

＜0.05）；平肝活血散中、高剂量组大鼠干预第7、14天的Longa评分均显著降低（

＜0.05），其余指标亦显著逆转（

＜0.05或

＜0.01）。

结论

平肝活血散可保护CIRI模型大鼠脑神经，抑制细胞凋亡；其机制可能与下调p53、Bax、Caspase-3蛋白表达，上调Bcl-2蛋白表达有关。

Abstract

OBJECTIVE

To investigate the effect and mechanism of Pinggan huoxue powder on cerebral ischemia-reperfusion injury （CIRI） in rats.

METHODS

CIRI model was induced by suture-occluded method. SD rats were divided into sham operation group， model group， positive control group （Tongxinluo capsule 0.325 g/kg）， Pinggan huoxue powder low-dose， medium-dose and high-dose groups （3.02， 6.04， 12.08 g/kg）， with 12 rats in each group. Each group was given constant volume of water or drug intragastrically， once a day， for consecutive 14 d. Longa score was used to evaluate neurological function of rats on the 1st， 7th and 14th day of intervention. After the last administration， the percentage of cerebral infarction volume in rats was measured， and neuronal injury （neuronal density） and neuronal apoptosis were observed； the protein expressions of p53， B cell lymphoma-2 （Bcl-2）， Bcl-2 associated X protein （Bax）， Caspase-3 and activated Caspase-3 （Cleaved caspase-3） in brain tissue were detected.

RESULTS

Compared with sham operation group， Longa score at the 1st， 7th and 14th day of intervention and the percentage of cerebral infarction volume in model group significantly increased （

＜0.05）， and the arrangement of cortical cells in ischemic side was disordered， Nissl bodies were absent and vacuolated， and a large number of cell apoptosis occurred. The neuronal density and protein expression of Bcl-2 at the lesion side were significantly decreased （

＜0.05）， while the protein expressions of p53， Bax， Caspase-3 and Cleaved Caspase-3 were significantly increased （

＜0.05 or

＜0.01）. Compared with model group， the expressions of Bcl-2 in cerebral ischemia cortex of rats in Pinggan huoxue powder high-dose group were significantly increased （

＜0.05）. Longa scores of rats in Pinggan huoxue powder medium-dose and high-dose groups on the 7th and 14th day of intervention were significantly decreased （

＜0.05）， and other indexes were also significantly reversed （

＜0.05 or

＜0.01）.

CONCLUSIONS

Pinggan huoxue powder can protect cerebral nerves and inhibit apoptosis of CIRI model rats， the mechanism of which may be related to down-regulating the protein expressions of p53， Bax and Caspase-3 and up-regulating the protein expression of Bcl-2.

关键词

平肝活血散脑缺血再灌注损伤细胞凋亡大鼠

Keywords

cerebral ischemia-reperfusion injuryapoptosisrat

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