Study on the effect of natural compound XCQ-9 of <italic style="font-style: italic">Cynanchum paniculatum</italic> on the proliferation and apoptosis of Jurkat cell and its mechanism

WEI Xuenai; YANG Kun; LIU Qin; ZHAO Peng; YAN Ying; LI Yanmei

doi:10.6039/j.issn.1001-0408.2023.01.09

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Study on the effect of natural compound XCQ-9 of Cynanchum paniculatum on the proliferation and apoptosis of Jurkat cell and its mechanism

更新时间：2023-02-22

- Study on the effect of natural compound XCQ-9 of Cynanchum paniculatum on the proliferation and apoptosis of Jurkat cell and its mechanism
- ZHONGGUO YAOFANG Vol. 34, Issue 1, Pages: 47-51(2023)
- 作者机构：
  
  1.贵州医科大学药学院，贵阳　550025
  2.贵州省中国科学院天然产物化学重点实验室，贵阳　550014
  3.贵州医科大学医药卫生管理学院，贵阳　550025
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.01.09
  CLC： R965
- Published：15 January 2023，
  
  Received：05 July 2022，
  
  Revised：15 December 2022，
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韦学耐,杨坤,刘琴等.徐长卿中的天然产物XCQ-9对Jurkat细胞增殖和凋亡的影响及机制研究 ^Δ[J].中国药房,2023,34(01):47-51.

WEI Xuenai,YANG Kun,LIU Qin,et al.Study on the effect of natural compound XCQ-9 of Cynanchum paniculatum on the proliferation and apoptosis of Jurkat cell and its mechanism[J].ZHONGGUO YAOFANG,2023,34(01):47-51.
韦学耐,杨坤,刘琴等.徐长卿中的天然产物XCQ-9对Jurkat细胞增殖和凋亡的影响及机制研究 ^Δ[J].中国药房,2023,34(01):47-51. DOI： 10.6039/j.issn.1001-0408.2023.01.09.

WEI Xuenai,YANG Kun,LIU Qin,et al.Study on the effect of natural compound XCQ-9 of Cynanchum paniculatum on the proliferation and apoptosis of Jurkat cell and its mechanism[J].ZHONGGUO YAOFANG,2023,34(01):47-51. DOI： 10.6039/j.issn.1001-0408.2023.01.09.

摘要

目的

探讨徐长卿中天然产物XCQ-9抑制人急性T淋巴细胞白血病Jurkat细胞增殖和凋亡的作用及可能机制。

方法

以Jurkat细胞作为白血病细胞模型，采用MTT法测定0（空白对照）、2.5、5、10、20、40 μmol/L XCQ-9作用24、48、72 h后对Jurkat细胞增殖的抑制作用。用0（空白对照）、2.5、5、10 μmol/L XCQ-9作用于Jurkat细胞24、48 h后，利用流式细胞术分析XCQ-9对细胞周期和细胞凋亡的影响，并通过Western blot实验检测上述药物作用24 h后细胞中胱天蛋白酶9（Caspase-9）、活化的Caspase-9（Cleaved Caspase-9）、Caspase-3、活化的Caspase-3（Cleaved Caspase-3）、聚腺苷二磷酸-核糖聚合酶（PARP）、活化的PARP（Cleaved PARP）、细胞周期蛋白依赖性激酶1（CDK1）和细胞周期蛋白B1（Cyclin B1）的表达情况。

结果

与空白对照比较，不同浓度XCQ-9均可显著降低Jurkat细胞的存活率（

＜0.01），并呈时间和浓度依赖性趋势。5、10 μmol/L XCQ-9作用48 h后均可显著诱导Jurkat细胞凋亡（

＜0.05或

＜0.01），将细胞周期阻滞在G

期（

＜0.01）。10 μmol/L XCQ-9作用24 h后，可显著下调细胞中CDK1、Caspase-9蛋白的表达（

＜0.01），上调细胞中Cyclin B1、Cleaved Caspase-9、Cleaved Caspase-3和Cleaved PARP蛋白的表达（

＜0.05或

＜0.01）。

结论

XCQ-9通过诱导G

期阻滞抑制Jurkat细胞增殖，并激活Caspase通路促进细胞凋亡，从而发挥其抗肿瘤作用。

Abstract

OBJECTIVE

To investigate the inhibitory effect of natural compound XCQ-9 of

Cynanchum paniculatum

on the proliferation and apoptosis of Jurkat cell line of human T-cell acute lymphoblastic leukemia and its possible mechanism.

METHODS

Jurkat cell was used as the leukemia cell model， and MTT assay was adopted to detect the inhibitory effects of 0（blank control）， 2.5， 5， 10， 20 and 40 μmol/L XCQ-9 on the proliferation of Jurkat cell after treated for 24， 48， 72 h. After treated with 0 （blank control）， 2.5， 5， 10 μmol/L XCQ-9 for 24 h and 48 h， the cell cycle and apoptosis were analyzed by flow cytometry. The expressions of Caspase-9， Cleaved Caspase-9， Caspase-3， Cleaved Caspase-3， poly ADP-ribose poly-merase （PARP）， Cleaved-PARP， cyclin-dependent kinase 1 （CDK1） and Cyclin B1 were detected by Western blot after treated for 24 h.

RESULTS

Compared with blank control group， XCQ-9 at different concentrations could significantly decrease the survival rate of Jurkat cells （

＜0.01）， and showed a dose and time-dependent manner. After 48 h treatment of 5， 10 μmol/L XCQ-9， Jurkat cell apoptosis was induced significantly （

＜0.05 or

＜0.01）， and the cell was arrested in G

phase （

＜0.01）. After 24 h treatment of 10 μmol/L XCQ-9， the protein expressions of CDK1 and Caspase-9 were remarkably down-regulated （

＜0.01）， while the protein expressions of Cyclin B1， Cleaved Caspase-9， Cleaved Caspase-3 and Cleaved PARP were significantly up-regulated （

＜0.05 or

＜0.01）.

CONCLUSIONS

XCQ-9 plays anti-tumor effect through inducing G

phase arrest to inhibit proliferation and activating Caspase pathway to increase apoptosis.

关键词

XCQ-9徐长卿人急性T淋巴细胞白血病Jurkat细胞细胞凋亡细胞周期胱天蛋白酶途径

Keywords

Cynanchum paniculatumhuman T-cell acute lymphoblastic leukemiaJurkat cellcell apoptosiscell cycleCaspase pathway

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Study on the effect of natural compound XCQ-9 of Cynanchum paniculatum on the proliferation and apoptosis of Jurkat cell and its mechanism

DOI：10.6039/j.issn.1001-0408.2023.01.09

摘要

Abstract

关键词

Keywords

references