Optimization of extraction technology for couplet medicinals of <italic style="font-style: italic">Astragalus membranaceus</italic>-<italic style="font-style: italic">Puerariae lobata</italic> by multi-index comprehensive scoring method combined with orthogonal experiment

WANG Dandan; NING Meng; WU Zuomin; WANG Rui; JIN Shaoju; YU Xiaotao

doi:10.6039/j.issn.1001-0408.2023.01.11

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Optimization of extraction technology for couplet medicinals of Astragalus membranaceus-Puerariae lobata by multi-index comprehensive scoring method combined with orthogonal experiment

更新时间：2023-02-22

- Optimization of extraction technology for couplet medicinals of Astragalus membranaceus-Puerariae lobata by multi-index comprehensive scoring method combined with orthogonal experiment
- ZHONGGUO YAOFANG Vol. 34, Issue 1, Pages: 57-61(2023)
- 作者机构：
  
  1.漯河市中心医院药学部，河南漯河　462300
  2.漯河医学高等专科学校药理学教研室，河南漯河　462002
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.01.11
  CLC： R943;R283
- Published：15 January 2023，
  
  Received：08 June 2022，
  
  Revised：20 October 2022，
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王单单,宁萌,吴作敏等.多指标综合评分法结合正交实验优选黄芪-葛根药对提取工艺^Δ[J].中国药房,2023,34(01):57-61.

WANG Dandan,NING Meng,WU Zuomin,et al.Optimization of extraction technology for couplet medicinals of Astragalus membranaceus-Puerariae lobata by multi-index comprehensive scoring method combined with orthogonal experiment[J].ZHONGGUO YAOFANG,2023,34(01):57-61.
王单单,宁萌,吴作敏等.多指标综合评分法结合正交实验优选黄芪-葛根药对提取工艺^Δ[J].中国药房,2023,34(01):57-61. DOI： 10.6039/j.issn.1001-0408.2023.01.11.

WANG Dandan,NING Meng,WU Zuomin,et al.Optimization of extraction technology for couplet medicinals of Astragalus membranaceus-Puerariae lobata by multi-index comprehensive scoring method combined with orthogonal experiment[J].ZHONGGUO YAOFANG,2023,34(01):57-61. DOI： 10.6039/j.issn.1001-0408.2023.01.11.

摘要

目的

优选黄芪-葛根药对的提取工艺。

方法

以提取液中葛根素、大豆苷、毛蕊异黄酮葡萄糖苷、大豆苷元、毛蕊异黄酮、芒柄花黄素的含量及出膏率作为评价指标，采用层次分析法确定各评价指标的权重系数并计算综合评分。通过单因素实验考察料液比、提取次数、提取时间对综合评分的影响，确定各因素的水平。采用多指标综合评分法，以上述7个指标的综合评分为指标，设计正交实验优选黄芪-葛根药对的提取工艺参数并进行验证。

结果

葛根素、大豆苷、毛蕊异黄酮葡萄糖苷、大豆苷元、毛蕊异黄酮、芒柄花黄素的含量及出膏率的权重系数分别为0.304 7、0.065 2、0.185 8、0.185 8、0.107 8、0.107 8、0.042 7。最终优选出的黄芪-葛根药对提取工艺为料液比1∶8（g/mL），加热回流提取3次，每次1 h。验证实验中各评价指标的RSD均小于3.00%（

＝3）。

结论

本研究优选的黄芪-葛根药对的提取工艺稳定、可行。

Abstract

OBJECTIVE

To optimize extraction technology of couplet medicinals of

Astragalus membranaceus

Puerariae lobatae

METHODS

With contents of puerarin，daidzin，calycosin-7-

-D-glucopyranoside，daidzein，calycosin and formononetin and the yield of dry extract as index，the analytic hierarchy method was used to determine the weight coefficient of each index and calculate the comprehensive score. The effects of solid-liquid ratio， extraction times and extraction time on the comprehensive score were investigated by single factor test. The level of each factor was determined. By multi-index comprehensive scoring method， using comprehensive scores of above 7 indexes as indexes，the extraction technology of couplet medicinals of

A. membranaceus

P. lobata

was optimized by orthogonal experiment，and the validation tests were conducted.

RESULTS

The weight coefficient for the contents of puerarin，daidzin，calycosin-7-

-D-glucopyranoside，daidzein，calycosin and formononetin and the yield of dry extract were respectively 0.304 7，0.065 2，0.185 8，0.185 8，0.107 8，0.107 8 and 0.042 7. The optimal extraction technology was determined as follows： solid-liquid of 1∶8（g/mL），extracting 3 times and for 1 h each time. RSD of each evaluation index in the validation test results was lower than 3.00% （

＝3）.

CONCLUSIONS

The optimized extraction technology for

A. membranaceus

P. lobata

is stable and feasible.

关键词

黄芪-葛根药对提取工艺多指标综合评分法正交实验葛根素大豆苷毛蕊异黄酮葡萄糖苷大豆苷元毛蕊异黄酮芒柄花黄素

Keywords

extraction technologymulti-index comprehensive scoring methodorthogonal experimentpuerarindaidzincalycosin-7-O-β-D-glucopyranosidedaidzeincalycosinformononetin

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Optimization of extraction technology for couplet medicinals of Astragalus membranaceus-Puerariae lobata by multi-index comprehensive scoring method combined with orthogonal experiment

DOI：10.6039/j.issn.1001-0408.2023.01.11

摘要

Abstract

关键词

Keywords

references