Study on inhibitory effects of Wubao capsule on airway inflammation in asthmatic model mice by regulating the upstream and downstream cytokines of type Ⅱ innate lymphoid cells

ZHANG Qiushi; HUANG Feng; TONG Xiaoyun; YANG Zhuya; LI Baojing; LIN Yuping; QU Lu; NIE Jian; HU Chunyan

doi:10.6039/j.issn.1001-0408.2023.02.06

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Study on inhibitory effects of Wubao capsule on airway inflammation in asthmatic model mice by regulating the upstream and downstream cytokines of type Ⅱ innate lymphoid cells

更新时间：2023-02-22

- Study on inhibitory effects of Wubao capsule on airway inflammation in asthmatic model mice by regulating the upstream and downstream cytokines of type Ⅱ innate lymphoid cells
- ZHONGGUO YAOFANG Vol. 34, Issue 2, Pages: 156-160(2023)
- 作者机构：
  
  1.云南中医药大学南药重点实验室，昆明　650500
  2.云南中医药大学第一附属医院科研部，昆明　650021
  3.云南中医药大学中药学院，昆明　650500
  4.云南中医药大学基础医学院，昆明　650500
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.02.06
  CLC： R965
- Published：30 January 2023，
  
  Received：28 September 2022，
  
  Revised：28 December 2022，
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张秋实,黄丰,童晓云等.五宝胶囊通过调节Ⅱ型固有淋巴细胞上下游细胞因子抑制哮喘模型小鼠气道炎症的研究 ^Δ[J].中国药房,2023,34(02):156-160.

ZHANG Qiushi,HUANG Feng,TONG Xiaoyun,et al.Study on inhibitory effects of Wubao capsule on airway inflammation in asthmatic model mice by regulating the upstream and downstream cytokines of type Ⅱ innate lymphoid cells[J].ZHONGGUO YAOFANG,2023,34(02):156-160.
张秋实,黄丰,童晓云等.五宝胶囊通过调节Ⅱ型固有淋巴细胞上下游细胞因子抑制哮喘模型小鼠气道炎症的研究 ^Δ[J].中国药房,2023,34(02):156-160. DOI： 10.6039/j.issn.1001-0408.2023.02.06.

ZHANG Qiushi,HUANG Feng,TONG Xiaoyun,et al.Study on inhibitory effects of Wubao capsule on airway inflammation in asthmatic model mice by regulating the upstream and downstream cytokines of type Ⅱ innate lymphoid cells[J].ZHONGGUO YAOFANG,2023,34(02):156-160. DOI： 10.6039/j.issn.1001-0408.2023.02.06.

摘要

目的

研究五宝胶囊通过调节Ⅱ型固有淋巴细胞（ILC2s）上下游细胞因子对哮喘模型小鼠气道炎症的影响。

方法

40只雌性BALB/c小鼠随机分为正常组、模型组、阳性对照组（地塞米松1 mg/kg）和五宝胶囊低、高剂量组（0.5、1 g/kg），每组8只，通过卵清蛋白（OVA）致敏、雾化激发的方法建立哮喘模型，并连续灌胃7 d生理盐水或药液。通过酶联免疫吸附试验检测血清中免疫球蛋白E（IgE）、OVA-IgE含量，支气管肺泡灌洗液（BALF）中白细胞介素5（IL-5）、IL-9、IL-13、IL-25、IL-33、胸腺基质淋巴细胞生成素（TSLP）、黏蛋白5AC（MUC5AC）含量；采用苏木精-伊红染色观察小鼠肺组织病理变化；采用过碘酸雪夫染色观察杯状细胞增生变化；通过流式细胞术检测肺组织中ILC2s的数量（除五宝胶囊低剂量组外）。

结果

与模型组比较，五宝胶囊高、低剂量组小鼠血清中IgE、OVA-IgE含量和BALF中IL-5、IL-9、IL-13、IL-25、IL-33、TSLP、MUC5AC含量均显著降低（

＜0.01），肺组织炎症细胞浸润及基底膜增厚均有不同程度的缓解，杯状细胞增生被抑制；五宝胶囊高剂量组小鼠肺组织中ILC2s数量显著降低（

＜0.01）。

结论

五宝胶囊能有效减少哮喘模型小鼠肺组织中ILC2s数量，降低小鼠BALF中ILC2s上下游细胞因子的含量，从而抑制气道炎症，改善哮喘。

Abstract

OBJECTIVE

To study the effects of Wubao capsule on airway inflammation in asthmatic model mice by regulating upstream and downstream cytokines of type Ⅱ innate lymphoid cells （ILC2s）.

METHODS

Totally 40 female BABL/c mice were randomly divided into normal group， model group， positive control group （dexamethasone 1 mg/kg）， Wubao capsule low-dose and high-dose groups （0.5， 1 g/kg）， with 8 mice in each group. Asthma models were induced by ovalbumin （OVA） sensitization and nebulization. Each group was given normal saline or drug intragastrically for 7 consecutive days. The contents of IgE and OVA-IgE in serum， the contents of interleukin 5 （IL-5）， IL-9， IL-13， IL-25， IL-33， thymic stromal lymphopoietin （TSLP） and mucin 5AC （MUC5AC） in bronchoalveolar lavage fluid （BALF） were determined by ELISA. HE staining was used to observe the pathological changes of lung tissues in mice. PAS staining was used to observe the changes of goblet cell proliferation in each group. The number of ILC2s in lung tissue was determined by flow cytometry （except for Wubao capsule low-dose group）.

RESULTS

Compared with model group， the contents of IgE and OVA-IgE in serum and the contents of IL-5， IL-9， IL-13， IL-25， IL-33， TSLP and MUC5AC in BALF were significantly reduced in Wubao capsule high-dose and low-dose groups （

＜0.01）. The infiltration of inflammatory cells and the thickening of basement membrane in lung tissue was alleviated to varying degrees， and the proliferation of goblet cells was inhibited； the number of ILC2s in lung tissues of mice in Wubao capsule high-dose group was significantly reduced （

＜0.01）.

CONCLUSIONS

Wubao capsule could effectively reduce the number of ILC2s in lung tissue， the contents of upstream and downstream cytokines of ILC2s in BALF of asthmatic model mice， so as to inhibit the airway inflammation and improve asthma.

关键词

五宝胶囊Ⅱ型固有淋巴细胞哮喘气道炎症

Keywords

type Ⅱ innate lymphoid cellsasthmaairway inflammation

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