OBJECTIVE

2

To prepare spider fibroin membrane loaded with

Periplaneta americana

extract， and investigate its characterization，

in vitro

drug release property and cytotoxicity.

METHODS

2

Using natural

spider silk collected from

Chilobrachys guangxiensis

as raw material，

P. americana

extract as model drug， the drug-loaded spider fibroin membrane （hereinafter referred to as drug-loaded membrane） was prepared by solvent casting method. The material matrix spider fibroin membrane without

P. americana

extract （hereinafter referred to as blank membrane） was prepared with same method. The membrane structure was characterized by static water contact angle， Fourier infrared chromatography， X-ray diffraction and scanning electron microscopy from different angles； drug release characteristics in artificial saliva were simulated

in vitro

to evaluate the drug sustained-release performance. MTT assay was adopted to validate the cytotoxicity of drug-loaded membrane.

RESULTS

2

The drug-loaded membrane was prepared， and the static water contact angle was less than 90°， which was less than that of blank membrane.

The drug-loaded membrane showed the characteristic absorption peak to polypeptide of

P. americana

extract at 1 500-1 700 cm

－1

.

X-ray diffraction and scanning electron microscopy also proved that the drug was successfully loaded into the pellicle. The release time of the pellicle in artificial saliva was more than 200 min. The MTT test results showed that the cell proliferation rates of blank membrane and drug-loaded membrane were 84.6% and 79.4% （both greater than 70%）， respectively， without significant potential cytotoxicity.

CONCLUSIONS

2

Drug-loaded membrane prepared with natural

spider silk has a certain sustained-release effect in artificial saliva， which can be further developed as a drug sustained-release carrier with excellent biological characteristics and biocompatibility.