Establishment of fingerprint and determination of differential components of <italic style="font-style: italic">Sophora flavescens</italic>

DONG Xiaolong; SHEN Jiajie; ZHU Jiayu; WANG Mengjiao; ZOU Lisi; PAN Linmei

doi:10.6039/j.issn.1001-0408.2023.03.09

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Establishment of fingerprint and determination of differential components of Sophora flavescens

更新时间：2023-02-22

- Establishment of fingerprint and determination of differential components of Sophora flavescens
- ZHONGGUO YAOFANG Vol. 34, Issue 3, Pages: 298-302(2023)
- 作者机构：
  
  1.南京中医药大学药学院，南京　210023
  2.江苏省植物药深加工工程研究中心，南京　210023
  3.江苏省中医药防治肿瘤协同创新中心，南京　210023
  4.江苏省经典名方工程研究中心，南京　210023
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.03.09
  CLC： R917
- Published：15 February 2023，
  
  Received：06 July 2022，
  
  Revised：26 November 2022，
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董晓龙,沈佳捷,朱佳钰等.苦参的指纹图谱建立及差异性成分含量测定 ^Δ[J].中国药房,2023,34(03):298-302.

DONG Xiaolong,SHEN Jiajie,ZHU Jiayu,et al.Establishment of fingerprint and determination of differential components of Sophora flavescens[J].ZHONGGUO YAOFANG,2023,34(03):298-302.
董晓龙,沈佳捷,朱佳钰等.苦参的指纹图谱建立及差异性成分含量测定 ^Δ[J].中国药房,2023,34(03):298-302. DOI： 10.6039/j.issn.1001-0408.2023.03.09.

DONG Xiaolong,SHEN Jiajie,ZHU Jiayu,et al.Establishment of fingerprint and determination of differential components of Sophora flavescens[J].ZHONGGUO YAOFANG,2023,34(03):298-302. DOI： 10.6039/j.issn.1001-0408.2023.03.09.

摘要

目的

建立不同产地苦参药材的指纹图谱，筛选差异性成分，并进行含量测定。

方法

采用《中药色谱指纹图谱相似度评价系统（2012版）》建立12批苦参药材的高效液相色谱（HPLC）指纹图谱，指认共有峰并进行相似度评价；采用SIMCA 14.1和SPSS 23.0软件进行化学模式识别分析[聚类分析（CA）、主成分分析（PCA）、正交偏最小二乘法-判别分析（OPLS-DA）]，并以变量重要性投影（VIP）值＞1为标准筛选影响苦参药材质量的差异性成分；采用同一HPLC法测定4种差异性成分的含量。

结果

12批苦参药材的指纹图谱中共有17个共有峰，相似度均大于0.96；共指认了6个共有峰，分别为氧化苦参碱（峰1）、氧化槐果碱（峰2）、苦参碱（峰10）、三叶豆紫檀苷（峰14）、苦参酮（峰16）、降苦参酮（峰17）。CA、PCA、OPLS-DA结果显示，12批苦参药材可按不同产地聚为3类：S1～S7（陕西商州区）聚为一类，S8～S10（河南伊川县）聚为一类，S11～S12（内蒙古赤峰市）聚为一类。苦参碱、降苦参酮、苦参酮、氧化槐果碱以及峰11、峰9所代表的化学成分的VIP值均大于1。12批苦参药材中苦参碱、降苦参酮、苦参酮、氧化槐果碱的含量分别为2.65～4.93、1.54～3.44、9.63～12.94、5.08～6.10 mg/g。

结论

本研究成功建立了苦参药材的HPLC指纹图谱，并结合化学模式识别分析筛选出6种差异性成分，可为该药材的质量控制提供参考。

Abstract

OBJECTIVE

To establish the fingerprint of

Sophora flavescens

， and to screen differential components and determine their contents.

METHODS

HPLC fingerprints of 12 batches of

S. flavescens

were established by using

Similarity Evaluation System of Chromatographic Fingerprints of TCM

（2012 edition）； common peaks were identified and their similarities were evaluated. Chemical pattern recognition analysis [cluster analysis （CA），principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis（OPLS-DA）]

were performed with SIMCA 14.1 and SPSS 23.0 software， and differential components which influenced the quality of

S. flavescens

were screen with variable importance in the projection（VIP）＞1 as standard. Meanwhile， the contents of 4 kinds of differential components were determined by the same HPLC method.

RESULTS

There were 17 common peaks in the fingerprints of 12 batches of

S. flavescens

，and their similarities were all higher than 0.96. A total of 6 common peaks were identified， i.e. oxymatrine （peak 1）， oxysophocarpine （peak 2）， matrine （peak 10）， trifolirhizin （peak 14）， kurarinone （peak 16） and norkurarinone （peak 17）. Results of CA， PCA and OPLS-DA showed that 12 batches of

S. flavescens

were divided into 3 categories according to producing area， i.e. S1-S7 （Shangzhou District of Shaanxi Province） were grouped into one category， S8-S10 （Yichuan County of Henan Province） into one category and S11-S12 （Chifeng City of Inner Mongolia） into one category. VIPs of matrine， norkurarinone， kurarinone and oxysophocarpine and the chemical components represented by peak 11 and 9 were all greater than 1. The contents of matrine， norkurarinone， kurarinone and oxysophocarpine in 12 batches of

S. flavescens

were 2.65-4.93， 1.54-3.44， 9.63-12.94 and 5.08-6.10 mg/g， respectively.

CONCLUSIONS

HPLC fingerprint of

S. flavescens

is established successfully in the study， and can be used to screen 6 differential components by combining with chemical pattern recognition analysis， which can provide reference for quality control of

S. flavescens

关键词

苦参指纹图谱含量测定化学模式识别分析

Keywords

fingerprintcontent determinationchemical pattern recognition analysis

references

王圳伊，王露露，张晶.苦参的化学成分、药理作用及炮制方法的研究进展[J].中国兽药杂志，2019，53（10）：71-79.

姚梅芬，张思巨，李琳，等.苦参中黄酮类成分及其药理作用研究现状[J].中国中医药信息杂志，2013，20（3）：110-112.

于娜，范红艳.苦参黄酮类化合物药理作用的研究进展[J].吉林医药学院学报，2021，42（4）：304-307.

胡婷，邹成梅，江吉周.苦丁茶主物质提取及其HPLC指纹图谱分析[J].华中师范大学学报（自然科学版），2021，55（1）：61-71.

梁霜，兰庆同，陈宗燕，等.全国中药特色技术传承项目在医院中药饮片验收中的应用[J].中成药，2019，41（9）：2274-2276.

刘东方，赵丽娜，李银峰，等.中药指纹图谱技术的研究进展及应用[J].中草药，2016，47（22）：4085-4094.

杜义龙，李赛，李艳荣，等.半枝莲标准汤剂的指纹图谱建立与抗氧化活性成分的化学模式识别[J].中国药房，2022，33（4）：425-432.

王梦娇，唐婷婷，潘林梅，等.基于HPLC指纹图谱和化学模式识别评价不同产地瓜蒌质量[J].中国现代应用药学，2021，38（8）：919-924.

李慧峰，孔祥鹏，孟霜，等.复方茴芹颗粒的指纹图谱建立、化学模式识别及多成分含量测定[J].中国药房，2022，33（21）：2627-2631.

宋奇，刘宏，王添敏，等. HPLC指纹图谱和多成分定量结合化学模式识别法评价不同产地胡桃楸叶质量[J].中草药，2022，53（7）：2143-2149.

李慧峰，田凡玉，孟霜，等.酒五味子的指纹图谱建立及化学模式识别分析[J].中国药房，2021，32（24）：3008-3013.

龚伟伟，罗光明，秦倩，等.茶芎HPLC指纹图谱的建立及差异性成分的含量测定[J].中国药房，2022，33（16）：1968-1973.

陈婷，胡芳，唐樑，等.基于HPLC指纹图谱及定量测定的益气逐瘀利水方质量评价研究[J].中国医院药学杂志，2022，42（7）：707-712.

潘宏春，阳勇，秦伟瀚，等.基于多元统计学方法的亚香棒虫草与冬虫夏草化学差异研究[J].中草药，2020，51（16）：4316-4324.

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Related Institution

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Establishment of fingerprint and determination of differential components of Sophora flavescens

DOI：10.6039/j.issn.1001-0408.2023.03.09

摘要

Abstract

关键词

Keywords

references