Difference analysis of chemical components in roots and rhizomes of 3 medicinal plants of <italic style="font-style: italic">Gentiana</italic> from the same origin

LI Rui; ZHANG Yingying; LIU Weihong; SONG Yanqiu; LI Longxing; LI Haifeng

doi:10.6039/j.issn.1001-0408.2023.03.10

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Difference analysis of chemical components in roots and rhizomes of 3 medicinal plants of Gentiana from the same origin

更新时间：2023-02-22

- Difference analysis of chemical components in roots and rhizomes of 3 medicinal plants of Gentiana from the same origin
- ZHONGGUO YAOFANG Vol. 34, Issue 3, Pages: 303-308(2023)
- 作者机构：
  
  1.大理大学药学院，云南大理　671003
  2.云南省滇西抗病原植物资源筛选研究重点实验室，云南大理　671003
  3.大理大学图书馆，云南大理　671003
  4.大理大学公共卫生学院，云南大理　671003
  5.大理大学分析测试中心，云南大理　671003
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.03.10
  CLC： R284.1;R917
- Published：15 February 2023，
  
  Received：12 August 2022，
  
  Revised：26 December 2022，
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李睿,张莹莹,刘卫红等.相同产地3种龙胆属植物根及根茎中化学成分的差异分析 ^Δ[J].中国药房,2023,34(03):303-308.

LI Rui,ZHANG Yingying,LIU Weihong,et al.Difference analysis of chemical components in roots and rhizomes of 3 medicinal plants of Gentiana from the same origin[J].ZHONGGUO YAOFANG,2023,34(03):303-308.
李睿,张莹莹,刘卫红等.相同产地3种龙胆属植物根及根茎中化学成分的差异分析 ^Δ[J].中国药房,2023,34(03):303-308. DOI： 10.6039/j.issn.1001-0408.2023.03.10.

LI Rui,ZHANG Yingying,LIU Weihong,et al.Difference analysis of chemical components in roots and rhizomes of 3 medicinal plants of Gentiana from the same origin[J].ZHONGGUO YAOFANG,2023,34(03):303-308. DOI： 10.6039/j.issn.1001-0408.2023.03.10.

摘要

目的

为从龙胆属植物中挖掘坚龙胆可替代资源提供依据。

方法

采用高效液相色谱（HPLC）法对3种龙胆属植物（坚龙胆、头花龙胆、微籽龙胆）根及根茎中4种活性成分（龙胆苦苷、獐牙菜苦苷、獐牙菜苷和苦龙胆酯苷）的含量进行测定；采用超高效液相色谱-电喷雾-四极杆飞行时间高分辨质谱联用（UPLC-ESI-Q-TOF-MS）技术对上述植物根及根茎中化学成分进行鉴定，并进行主成分分析（PCA）。

结果

头花龙胆与坚龙胆根及根茎中均含有龙胆苦苷、獐牙菜苦苷、獐牙菜苷和苦龙胆酯苷这4种活性成分，其中头花龙胆和坚龙胆根及根茎中龙胆苦苷含量均是2020年版《中国药典》（一部）限量标准的4倍以上；而微籽龙胆根及根茎中仅检测到獐牙菜苦苷、獐牙菜苷和苦龙胆酯苷，其中的獐牙菜苷和苦龙胆酯苷含量分别是坚龙胆的34.12、8.81倍。通过UPLC-ESI-Q-TOF-MS技术从3种龙胆属植物根及根茎中共鉴定出33种化合物，主要为环烯醚萜类成分；此外，坚龙胆和头花龙胆中还含有

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3.01623845

2.69857121

酮类成分，微籽龙胆中还含有黄酮类成分。PCA结果显示，头花龙胆和坚龙胆差异较小，而微籽龙胆与坚龙胆差异较大。

结论

同一产地头花龙胆与坚龙胆根及根茎的化学成分差异较小，存在可替代性；而微籽龙胆与坚龙胆根及根茎的化学成分差异较大，不能代替坚龙胆入药。

Abstract

OBJECTIVE

To provide reference for exploring alternative resources of

Gentiana rigescens

from the plants of

Gentiana

METHODS

The contents of four components （gentiopicroside， swertiamarin， swertioside and amarogentin） in the roots and rhizomes from 3 plants of

Gentiana

（

G. rigescens

，

G. cephalantha

，

G. delavayi

） were determined by high-performance liquid chromatography （HPLC）. The chemical compositions in the above roots and rhizomes were identified by ultra-performance liquid chromatography electrospray ionization quarter-time of flight mass spectrometry （UPLC-ESI-Q-TOF-MS）， and the differences were analyzed by principal component analysis （PCA）.

RESULTS

Four active components such as gentiopicroside， swertiamarin， swertioside and amarogentin were detected in the roots and rhizomes of

G. rigescens

and

G. cephalantha

， and the contents of the four components were similar in both. The contents of gentiopicroside in the root and rhizome of

G. cephalantha

and

G.rigescens

were more than four times of the limit standard of the

Chinese Pharmacopoeia

（Part Ⅰ） in 2020； However， only swertiamarin， swertioside and amarogentin were detected in the roots and rhizomes of

G.delavayi

， and the contents of swertioside and amarogentin were 34.12 and 8.81 times of those of

G.rigescens

， respectively. In addition， a total of 33 compounds were identified from the roots and rhizomes of 3 plants of

Gentiana

by UPLC-ESI-Q-TOF-MS， mainly iridoids. Additionally，

G. rigescens

and

G. cephalantha

contained xantones，

G. delavayi

contained flavonoids. PCA showed that there was a small difference between

G. rigescens

and

G. cephalantha

； however， there was a big difference between

G. delavayi

and

G. rigescens

CONCLUSIONS

The difference between the roots and rhizomes of

G. cephalantha

and

G. rigescens

from the same origin is small and there is substitutability； while the difference in the chemical components from roots and rhizomes between

G. delavayi

and

G. rigescens

is great and

G. delavayi

cannot be used as medicine instead of

G. rigescens

关键词

坚龙胆头花龙胆微籽龙胆环烯醚萜类成分化学成分含量测定鉴定

Keywords

Gentiana cephalanthaGentiana delavayiiridoidschemical componentscontent determinationidentification

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Difference analysis of chemical components in roots and rhizomes of 3 medicinal plants of Gentiana from the same origin

DOI：10.6039/j.issn.1001-0408.2023.03.10

摘要

Abstract

关键词

Keywords

references