Comparison of anti-ischemic stroke effect of different extraction parts from <italic style="font-style: italic">Gastrodia elata</italic>

XU Chunping; YANG Qian; MENG Qingting; YAN Mingli; NING Long; SUN Hang; HE Fangyan

doi:10.6039/j.issn.1001-0408.2023.04.09

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Comparison of anti-ischemic stroke effect of different extraction parts from Gastrodia elata

更新时间：2023-02-24

- Comparison of anti-ischemic stroke effect of different extraction parts from Gastrodia elata
- ZHONGGUO YAOFANG Vol. 34, Issue 4, Pages: 429-432(2023)
- 作者机构：
  
  云南中医药大学中药学院，昆明　650500
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.04.09
  CLC： R965;R285
- Published：28 February 2023，
  
  Received：06 October 2022，
  
  Revised：30 January 2023，
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许春萍,杨茜,孟庆婷等.天麻不同提取部位抗缺血性脑卒中作用比较 ^Δ[J].中国药房,2023,34(04):429-432.

XU Chunping,YANG Qian,MENG Qingting,et al.Comparison of anti-ischemic stroke effect of different extraction parts from Gastrodia elata[J].ZHONGGUO YAOFANG,2023,34(04):429-432.
许春萍,杨茜,孟庆婷等.天麻不同提取部位抗缺血性脑卒中作用比较 ^Δ[J].中国药房,2023,34(04):429-432. DOI： 10.6039/j.issn.1001-0408.2023.04.09.

XU Chunping,YANG Qian,MENG Qingting,et al.Comparison of anti-ischemic stroke effect of different extraction parts from Gastrodia elata[J].ZHONGGUO YAOFANG,2023,34(04):429-432. DOI： 10.6039/j.issn.1001-0408.2023.04.09.

摘要

目的

比较天麻不同提取部位的抗缺血性脑卒中（IS）作用，为筛选天麻抗IS的有效部位提供参考。

方法

采用乙醇回流提取、乙酸乙酯萃取法对天麻进行提取分离。以花生四烯酸颈内动脉注射诱导大鼠弥漫性脑血栓模型，以缺血侧脑组织中伊文思蓝（EB）含量为指标考察天麻生粉、天麻醇提取物、天麻醇提取残渣、天麻乙酸乙酯提取物、天麻乙酸乙酯提取残渣、天麻素以及阿司匹林（阳性对照药）的抗IS作用。

结果

与模型组比较，阿司匹林、天麻醇提取物、天麻乙酸乙酯提取物均可显著降低模型大鼠缺血侧脑组织中EB含量（

＜0.05）；天麻生粉有降低模型大鼠缺血侧脑组织中EB含量的趋势，但差异无统计学意义（

＞0.05）；天麻醇提取残渣、天麻乙酸乙酯提取残渣、天麻素对模型大鼠缺血侧脑组织中EB含量均几乎无影响。

结论

天麻醇提取物和天麻乙酸乙酯提取物均具有抗IS作用，且作用强于天麻生粉。

Abstract

OBJECTIVE

To compare anti-ischemic stroke （IS） effect of different extraction parts from

Gastrodia elata

， and to provide reference for screening the anti-IS effective parts of

G. elata

METHODS

G. elata

was extracted and separated by ethanol reflux extraction and ethyl acetate extraction. The rat model of diffuse cerebral thrombosis was induced by internal carotid artery injection of arachidonic acid （AA）； the anti-IS effect of

G. elata

powder， ethanol extract of

G. elata

， residue of ethanol extract of

G. elata

， ethyl acetate extract of

G. elata

， residue of ethyl acetate extract of

G. elata

， gastrodin and aspirin （positive control drug） were investigated with the content of Evans blue （EB） in the ischemic brain tissue as index.

RESULTS

Compared with model group， aspirin， ethanol extract of

G. elata

and ethyl acetate extract of

G. elata

could significantly decrease the content of EB in the ischemic brain tissue of model rats （

＜0.05）.

G. elata

powder had the tendency to reduce the content of EB in the ischemic brain tissue of model rats， without statistical significance （

＞0.05）. The residue of ethanol extract of

G. elata

， residue of ethyl acetate extract of

G. elata

and gastrodin had little effect on the content of EB in the ischemic brain tissue of model rats.

CONCLUSIONS

Both ethanol extract of

G. elata

and ethyl acetate extract of

G. elata

have anti-IS effects， which are stronger than that of

G. elata

powder.

关键词

天麻缺血性脑卒中提取部位药效学有效部位

Keywords

ischemic strokeextraction partpharmacodynamicseffective part

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Comparison of anti-ischemic stroke effect of different extraction parts from Gastrodia elata

DOI：10.6039/j.issn.1001-0408.2023.04.09

摘要

Abstract

关键词

Keywords

references