OBJECTIVE

2

To study the extraction and enrichment technology of chrysosplenides A （CA） and I （CI） in Tibetan medicine

Chrysosplenium axillare

.

METHODS

2

HPLC method was used to determine the contents of CA and CI. The orthogonal experiment was used to optimize the extraction technology of CA and CI in

C. axillare

using total transfer rate of CA and CI as evaluation indexes， with volume fraction of ethanol， extraction temperature， extraction times and solid-liquid ratio as factors. The validation test was also performed. The enrichment technology of CA and CI in

C. axillare

was optimized using D101 macroporous adsorption resin as adsorbent， total contents of CA and CI as evaluation indexes， with the volume fraction and dosage of eluent for impurities and target components. The validation test was also performed.

RESULTS

2

The optimum extraction conditions of CA and CI from

C. axillare

were as follows： the medicinal powder of

C. axillare

was extracted by ultrasound at room temperature for 45 min at one time with 8 times of 50% ethanol. Results of validation tests showed that total transfer rate of CA and CI in

C. axillare

was 95.43% in average （RSD＝1.02%，

n

＝3）. The optimal enrichment technology was as follows： the sample solution was added into D101 macroporous adsorption resin column and stood for 1 hour； the impurities were eluted with 20% ethanol 4 BV （column volume）， and CA and CI were eluted with 50% ethanol 4 BV. The results of validation tests showed that total content of CA and CI was 322.7 mg/g in average （RSD＝1.05%，

n

＝3）， with average enrichment multiple of 11.61 times.

CONCLUSIONS

2

The study has successfully optimized the extraction and enrichment technology of CA and CI from

C. axillare

， and can provide reference for the development and utilization of CA and CI.