Protective effects of twin drug ST-11 against PC12 cell injury induced by oxygen-glucose deprivation/reperfusion and its mechanism

WEN Jinlan; CHEN Lina; ZHANG Shanhui; LU Jun; WU Shuxia; ZHANG Jinjuan; DONG Yongxi; DONG Li

doi:10.6039/j.issn.1001-0408.2023.06.04

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Protective effects of twin drug ST-11 against PC12 cell injury induced by oxygen-glucose deprivation/reperfusion and its mechanism

更新时间：2023-03-24

- Protective effects of twin drug ST-11 against PC12 cell injury induced by oxygen-glucose deprivation/reperfusion and its mechanism
- ZHONGGUO YAOFANG Vol. 34, Issue 6, Pages: 660-665(2023)
- 作者机构：
  
  1.贵州医科大学药学院，贵阳　550025
  2.贵州医科大学基础医学院，贵阳　550025
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.06.04
  CLC： R965
- Published：30 March 2023，
  
  Received：31 August 2022，
  
  Revised：15 December 2022，
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文金兰,陈莉娜,张善惠等.孪药ST-11对氧糖剥夺/再灌注致PC12细胞损伤的保护作用及机制 ^Δ[J].中国药房,2023,34(06):660-665.

WEN Jinlan,CHEN Lina,ZHANG Shanhui,et al.Protective effects of twin drug ST-11 against PC12 cell injury induced by oxygen-glucose deprivation/reperfusion and its mechanism[J].ZHONGGUO YAOFANG,2023,34(06):660-665.
文金兰,陈莉娜,张善惠等.孪药ST-11对氧糖剥夺/再灌注致PC12细胞损伤的保护作用及机制 ^Δ[J].中国药房,2023,34(06):660-665. DOI： 10.6039/j.issn.1001-0408.2023.06.04.

WEN Jinlan,CHEN Lina,ZHANG Shanhui,et al.Protective effects of twin drug ST-11 against PC12 cell injury induced by oxygen-glucose deprivation/reperfusion and its mechanism[J].ZHONGGUO YAOFANG,2023,34(06):660-665. DOI： 10.6039/j.issn.1001-0408.2023.06.04.

摘要

目的

研究川芎嗪-灯盏乙素苷元孪药ST-11对氧糖剥夺/再灌注（OGD/R）致大鼠肾上腺髓质嗜铬细胞瘤PC12细胞损伤的保护作用及机制。

方法

将细胞分为空白组、模型组、尼莫地平组（阳性对照，5 μmol/L）、ST-11不同浓度组（5、10、20 μmol/L）。预先给药干预24 h后，除空白组外的其余各组细胞均以含10 mmol/L Na

的无糖DMEM培养基缺糖缺氧培养4 h，待复糖复氧培养4 h后，检测各组细胞的存活率，细胞上清液中乳酸脱氢酶（LDH）、过氧化氢酶（CAT）、谷胱甘肽（GSH）、丙二醛（MDA）、超氧化物歧化酶（SOD）的含量，细胞凋亡率，细胞活性氧（ROS）和线粒体膜电位（MMP）水平，以及B细胞淋巴瘤2相关X蛋白（Bax）、B细胞淋巴瘤2（Bcl-2）及胱天蛋白酶3（caspase-3）蛋白的表达情况。

结果

与空白组比较，模型组的细胞存活率，上清液中CAT、GSH和SOD含量，细胞MMP水平，细胞Bcl-2蛋白的相对表达量和Bcl-2/Bax比值均显著降低（

＜0.05），而上清液中LDH、MDA含量，细胞ROS水平，细胞凋亡率，细胞Bax、caspase-3蛋白的相对表达量均显著升高（

＜0.05）。与模型组比较，ST-11各浓度组上述指标（5 μmol/L ST-11组caspase-3蛋白除外）均显著逆转（

＜0.05）。

结论

ST-11对OGD/R致PC12细胞损伤具有一定的保护作用，且作用可能与减少细胞氧化应激、抑制细胞凋亡有关。

Abstract

OBJECTIVE

To study the protective effects of ligustrazine-scutellarein twin drug ST-11 on rat adrenal medullary pheochromocytoma PC12 cell injury induced by oxygen-glucose deprivation/reperfusion （OGD/R） and its mechanism.

METHODS

PC12 cells were divided into blank group， model group， nimodipine group （positive control， 5 μmol/L） and different concentration groups of ST-11 （5， 10， 20 μmol/L）. After 24 hours of pre-administration intervention， all the other groups except the blank group were cultured in glucose-free DMEM culture medium containing 10 mmol/L Na

for 4 hours with glucose deficiency and hypoxia. After 4 hours of glucose and oxygen re-introduction， the survival rate of cells in each group， the contents of lactate dehydrogenase （LDH）， catalase （CAT）， glutathione （GSH）， malondialdehyde （MDA） and superoxide dismutase （SOD） in cell supernatant， apoptosis rate， the levels of reactive oxygen species （ROS） and mitochondrial membrane potential （MMP）， the protein expressions of B-cell lymphoma 2 related X protein （Bax）， B-cell lymphoma 2 （Bcl-2） and caspase-3 were all detected in each group.

RESULTS

Compared with blank group， the cell survival rate， the contents of CAT， GSH and SOD in cell supernatant， MMP level， relative expression of Bcl-2 and Bcl-2/Bax ratio in model group decreased significantly （

＜0.05）， while the contents of LDH and MDA， ROS level， apoptosis rate， relative expressions of Bax and caspase-3 were significantly increased （

＜0.05）. Compared with model group， above indexes of ST-11 groups （except for the protein expression of caspase-3 in 5 μmol/L ST-11 group） were reversed signifi-cantly （

＜0.05）.

CONCLUSIONS

ST-11 has a certain protec-tive effect on OGD/R-injured PC12 cells， and its effects may be related to reduction of oxidative stress and inhibition of cell apoptosis.

关键词

ST-11川芎嗪灯盏乙素苷元孪药氧糖剥夺/再灌注氧化应激细胞凋亡PC12细胞

Keywords

ligustrazinescutellareintwin drugoxygen-glucose deprivation/reperfusionoxidative stresscell apoptosisPC12 cells

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