Effect and mechanism of baicalin on the proliferation and migration of human periodontal ligament stem cells induced by lipopolysaccharide

WANG Qiao; FENG Bo; GAO Yongzhi

doi:10.6039/j.issn.1001-0408.2023.10.12

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Effect and mechanism of baicalin on the proliferation and migration of human periodontal ligament stem cells induced by lipopolysaccharide

更新时间：2023-05-25

- Effect and mechanism of baicalin on the proliferation and migration of human periodontal ligament stem cells induced by lipopolysaccharide
- ZHONGGUO YAOFANG Vol. 34, Issue 10, Pages: 1216-1222(2023)
- 作者机构：
  
  1.齐齐哈尔医学院附属第二医院口腔科，黑龙江齐齐哈尔　161006
  2.齐齐哈尔市五官医院口腔科，黑龙江齐齐哈尔　161006
  3.齐齐哈尔医学院附属第一医院口腔科，黑龙江齐齐哈尔　161041
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.10.12
  CLC： R965
- Published：30 May 2023，
  
  Received：14 November 2022，
  
  Revised：10 March 2023，
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王桥,冯博,高永志.黄芩苷对脂多糖诱导的人牙周膜干细胞增殖、迁移作用及机制 ^Δ[J].中国药房,2023,34(10):1216-1222.

WANG Qiao,FENG Bo,GAO Yongzhi.Effect and mechanism of baicalin on the proliferation and migration of human periodontal ligament stem cells induced by lipopolysaccharide[J].ZHONGGUO YAOFANG,2023,34(10):1216-1222.
王桥,冯博,高永志.黄芩苷对脂多糖诱导的人牙周膜干细胞增殖、迁移作用及机制 ^Δ[J].中国药房,2023,34(10):1216-1222. DOI： 10.6039/j.issn.1001-0408.2023.10.12.

WANG Qiao,FENG Bo,GAO Yongzhi.Effect and mechanism of baicalin on the proliferation and migration of human periodontal ligament stem cells induced by lipopolysaccharide[J].ZHONGGUO YAOFANG,2023,34(10):1216-1222. DOI： 10.6039/j.issn.1001-0408.2023.10.12.

摘要

目的

探究黄芩苷对脂多糖（LPS）诱导的人牙周膜干细胞（hPDLSCs）增殖、迁移及Janus蛋白酪氨酸激酶2（JAK2）/信号转导和转录激活因子3（STAT3）信号通路的调控作用。

方法

将hPDLSCs分为对照组、LPS组、黄芩苷不同浓度（0.1、1、10 mg/L）组，采用ELISA法和CCK-8法测定细胞炎症因子[白细胞介素6（IL-6）、IL-1β和肿瘤坏死因子α（TNF-α）]含量和细胞活力，以筛选最适黄芩苷浓度并进行后续通路验证实验。随后将细胞分为对照组、LPS组、最适黄芩苷浓度组、抑制剂组（10 μg/mL LPS+1 mg/L黄芩苷＋3 μmol/L JAK2/STAT3通路抑制剂AG490），干预24 h后检测hPDLSCs增殖率、凋亡率、迁移率、侵袭细胞数、细胞周期素D1（Cyclin D1）、胱天蛋白酶3（caspase-3）mRNA和蛋白表达及JAK2/STAT3信号通路相关蛋白表达。

结果

根据细胞炎症因子含量和细胞活力结果选择1 mg/L为黄芩苷最适浓度。与对照组比较，LPS组细胞增殖率、迁移率、侵袭细胞数、Cyclin D1 mRNA及蛋白表达水平显著降低，细胞凋亡率、caspase-3 mRNA及蛋白表达、p-JAK2和p-STAT3蛋白表达显著升高（

＜0.05）；经1 mg/L黄芩苷干预后，上述指标均得到显著改善（

＜0.05）；而抑制剂组上述指标的改善程度更加显著（

＜0.05）。

结论

黄芩苷可通过抑制JAK2/STAT3信号通路来促进LPS诱导的hPDLSCs增殖、迁移和侵袭，抑制其凋亡及炎症反应。

Abstract

OBJECTIVE

To explore the regulatory effects of baicalin on the proliferation and migration of human periodontal ligament stem cells （hPDLSCs） induced by lipopolysaccharide （LPS） and Janus protein tyrosine kinase 2 （JAK2）/signal transduction and transcription activator 3 （STAT3） signaling pathways.

METHODS

hPDLSCs were divided into control group， LPS group， different concentration baicalin groups （0.1， 1 and 10 mg/L）. ELISA method and CCK-8 assay were used to determine the contents of cell inflammatory factors [interleukin 6 （IL-6）， IL-1β and tumor necrosis factor-α （TNF-α）] and cell viability， so as to screen the optimal concentration of baicalin for follow-up pathway validation experiments. The cells were then divided into control group， LPS group， optimal baicalin concentration group and inhibitor group （10 μg/mL LPS+1 mg/L baicalin +3 μmol/L JAK2/STAT3 pathway inhibitor AG490）. After treated for 24 h， the proliferation rate of hPDLSCs， apoptosis rate， migration rate， invasion cell number， mRNA and protein expressions of Cyclin D1 and caspase-3， the expression of JAK2/STAT3 pathway-related proteins were all detected.

RESULTS

According to cell inflammatory factors and cell viability， 1 mg/L was selected as the optimal concentration of baicalin. Compared with control group， cell proliferation rate， migration rate， invasion cell number， Cyclin D1 mRNA and protein expression were significantly decreased in LPS group， while cell apoptosis rate， caspase-3 mRNA and protein expression， p-JAK2 and p-STAT3 protein expression were significantly increased （

＜0.05）. After treated with 1 mg/L baicalin， the above indexes were reversed significantly （

＜0.05）. The improvement of above indexes in the inhibitor group was more obvious （

＜0.05）.

CONCLUSIONS

Baicalin can promote the proliferation， migration and invasion of LPS-induced hPDLSCs and inhibit their apoptosis and inflammation by blocking the JAK2/STAT3 pathway.

关键词

牙周炎黄芩苷牙周膜干细胞Janus蛋白酪氨酸激酶2/信号转导和转录激活因子3信号通路增殖迁移

Keywords

baicalinperiodontal ligament stem cellsJanus protein tyrosine kinase 2/signal transduction and transcription activator 3 signaling pathwayproliferationmigration

references

BARTOLD P M. Lifestyle and periodontitis：the emergence of personalized periodontics[J]. Periodontol 2000，2018，78（1）：7-11.

KWON T，LAMSTER I B，LEVIN L. Current concepts in the management of periodontitis[J]. Int Dent J，2021，71（6）：462-476.

TOMOKIYO A，WADA N，MAEDA H. Periodontal ligament stem cells：regenerative potency in periodontium[J]. Stem Cells Dev，2019，28（15）：974-985.

CRUZ MARTÍNEZ C，DIAZ GÓMEZ M，OH M S. Use of traditional herbal medicine as an alternative in dental treatment in Mexican dentistry：a review[J]. Pharm Biol，2017，55（1）：1992-1998.

CHANAJ-KACZMAREK J，OSMAŁEK T，SZYMAŃSKA E，et al. Development and evaluation of thermosensitive hydrogels with binary mixture of Scutellariae baicalensis radix extract and chitosan for periodontal diseases treatment[J]. Int J Mol Sci，2021，22（21）：11319.

毛友兵，王洒. 基于JAK/STAT3信号转导通路探讨黄芩苷预处理对糖尿病大鼠心肌缺血再灌注损伤的保护作用[J]. 中国中医急症，2019，28（7）：1180-1183.

李月. 利拉鲁肽通过JAK2/STAT3信号通路影响糖尿病牙周炎的发生发展[D]. 兰州：兰州大学，2020.

喻茂文，柳建磊，汤洪波，等. LINC00963靶向miR-525-5p调控LPS诱导的牙周膜细胞损伤的机制研究[J]. 实用口腔医学杂志，2022，38（4）：455-460.

黄琼，李婧，李长宏. 黄芩苷对人牙周膜干细胞生物学特性的影响及作用机制[J]. 西部医学，2021，33（10）：1463-1467.

梁又德，刘鑫，宋大为，等. 白血病抑制因子通过JAK2/STAT3信号通路对牙周膜细胞增殖影响的研究[J]. 临床口腔医学杂志，2019，35（6）：335-339.

CARVALHO C V，SARAIVA L，BAUER F P F，et al. Orthodontic treatment in patients with aggressive perio-dontitis[J]. Am J Orthod Dentofacial Orthop，2018，153（4）：550-557.

卢惠冰，徐雄均，杨伟湘，等. 黄芩苷口腔缓释膜辅助治疗慢性牙周炎临床观察[J]. 新中医，2018，50（11）：156-159.

HUANG T，LIU Y N，ZHANG C L. Pharmacokinetics and bioavailability enhancement of baicalin：a review[J]. Eur J Drug Metab Pharmacokinet，2019，44（2）：159-168.

BAILLY C. The implication of the PD-1/PD-L1 checkpoint in chronic periodontitis suggests novel therapeutic opportunities with natural products[J]. Jpn Dent Sci Rev，2020，56（1）：90-96.

LU J J，HU Y J，TANG Z Y，et al. Porphyromonas gingivalis lipopolysaccharide enhances the proliferation of human periodontal ligament cells via upregulation of cyclin D1，cyclin A and cyclin B1[J]. Exp Ther Med，2022，23（1）：2.

WU Y Q，ZHUANG J B，ZHAO D，et al. Interaction between caspase-3 and caspase-5 in the stretch-induced programmed cell death in the human periodontal ligament cells[J]. J Cell Physiol，2019，234（8）：13571-13581.

徐秋，邱新毓，高洁，等. 骨髓间充质干细胞外泌体调控STAT3通路促进牙周膜干细胞成骨分化[J]. 口腔生物医学，2022，13（1）：35-39.

张晨，刘珺，赵涵，等. Janus蛋白酪氨酸激酶2/信号转导和转录激活因子3信号转导通路在人慢性根尖周炎中的表达[J]. 口腔医学研究，2019，35（1）：84-88.

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