Study on the pharmacodynamics and mechanism of <italic style="font-style: italic">Blumea balsamifera</italic> total flavonoids against acute myocardial infarction model rats

LYU Yiting; WANG Yichen; FENG Yidong; SHI Zhongfeng; HAN Liang; ZHANG Xiaoqi

doi:10.6039/j.issn.1001-0408.2023.11.09

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Study on the pharmacodynamics and mechanism of Blumea balsamifera total flavonoids against acute myocardial infarction model rats

更新时间：2023-06-09

- Study on the pharmacodynamics and mechanism of Blumea balsamifera total flavonoids against acute myocardial infarction model rats
- ZHONGGUO YAOFANG Vol. 34, Issue 11, Pages: 1332-1336(2023)
- 作者机构：
  
  1.广东药科大学新药研发中心，广州　510006
  2.深圳海王医药科技研究院有限公司，广东深圳　518057
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.11.09
  CLC： R965
- Published：15 June 2023，
  
  Received：01 November 2022，
  
  Revised：13 April 2023，
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吕依婷,王溢晨,冯贻东等.艾纳香总黄酮对急性心肌梗死模型大鼠的药效作用及机制研究 ^Δ[J].中国药房,2023,34(11):1332-1336.

LYU Yiting,WANG Yichen,FENG Yidong,et al.Study on the pharmacodynamics and mechanism of Blumea balsamifera total flavonoids against acute myocardial infarction model rats[J].ZHONGGUO YAOFANG,2023,34(11):1332-1336.
吕依婷,王溢晨,冯贻东等.艾纳香总黄酮对急性心肌梗死模型大鼠的药效作用及机制研究 ^Δ[J].中国药房,2023,34(11):1332-1336. DOI： 10.6039/j.issn.1001-0408.2023.11.09.

LYU Yiting,WANG Yichen,FENG Yidong,et al.Study on the pharmacodynamics and mechanism of Blumea balsamifera total flavonoids against acute myocardial infarction model rats[J].ZHONGGUO YAOFANG,2023,34(11):1332-1336. DOI： 10.6039/j.issn.1001-0408.2023.11.09.

摘要

目的

研究艾纳香总黄酮对急性心肌梗死（AMI）模型大鼠的药效作用及可能机制。

方法

采用结扎SD大鼠冠状动脉左前降支的方法建立AMI模型。选取造模成功的50只大鼠，按照随机数字表法分为模型组（0.8%羧甲基纤维素溶液）、阳性对照组（复方丹参片，300 mg/kg）和艾纳香总黄酮低、中、高剂量组（3、10、30 mg/kg），每组10只；另取10只作为假手术组（0.8%羧甲基纤维素溶液）。手术1 d后开始灌胃给药，给药体积均为3 mL/kg，每天给药1次，连续4周。记录术前、术后及每周灌胃给药后大鼠心电图S-T段的变化；检测大鼠血流动力学指标[动脉收缩压（SBP）、舒张压（DBP）、平均动脉压（MAP）、左心室收缩压（LVSP）、左心室舒张末压（LVEDP）、左心室内压最大上升速率（+LVdp/dt

max

）和左心室内压最大下降速率（－LVdp/dt

max

）]；检测大鼠血清心肌酶[乳酸脱氢酶（LDH）、肌酸激酶同工酶-MB（CK-MB）]

和炎症因子[肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）、IL-1β]水平；检测大鼠心肌梗死率和心肌组织中磷脂酰肌醇3激酶（PI3K）、蛋白激酶B（Akt）的磷酸化水平。

结果

与模型组比较，给药后各组大鼠心电图S-T段均显著降低（

＜0.05）；艾纳香总黄酮中、高剂量组大鼠SBP、DBP、MAP、LVSP、+LVdp/dt

max

、－LVdp/dt

max

及p-PI3K

Tyr607

/PI3K、p-Akt

Thr308

/Akt、p-Akt

ser473

/Akt比值均显著升高（

＜0.05），LVEDP、血清心肌酶和炎症因子水平、心肌梗死率均显著降低（

＜0.05）。

结论

艾纳香总黄酮能改善AMI模型大鼠的心功能；其作用机制与抑制炎症因子表达，激活PI3K/Akt信号通路有关。

Abstract

METHODS

AMI model of SD rats was established by ligating anterior descending branch of left coronary artery. Fifty model rats were randomly divided into model group （0.8% carboxymethyl cellulose solution）， positive control group （Compound danshen tablet， 300 mg/kg），

B. balsamifera

total flavonoids low-dose， medium-dose and high-dose groups （3， 10， 30 mg/kg）， with 10 rats in each group. Other 10 rats were included in sham operation group （0.8% carboxymethyl cellulose solution）. After 1 day of surgery， they were given relevant medicine 3 mL/kg intragastrically， once a day， for 4 consecutive weeks. The changes of S-T segment were recorded before and after operation， after weekly intragastric administration. The hemodynamic indexes of rats were all determined， i.e. systolic blood pressure （SBP）， diastolic blood pressure （DBP）， mean arterial pressure （MAP）， left ventricular systolic pressure （LVSP）， left ventricular end diastolic blood pressure （LVEDP）， maximal left ventricular pressure rising rate （+LVdp/dt

max

）， maximal left ventricular pressure decreasing rate （－LVdp/dt

max

）. The levels of serum myocardial enzymes [lactate dehydrogenase （LDH）， creatine kinase isoenzyme-MB （CK-MB）]

and inflammatory factors [tumor necrosis factor-α （TNF-α）， interleukin-6 （IL-6）， IL-1β] were determined. The myocardial infarction rate of rats and the phosphorylation levels of phosphoinositide 3-kinase （PI3K） and protein kinase B （Akt） proteins in myocardial tissue were determined.

RESULTS

Compared with model group， S-T segments of electrocardiogram were all decreased significantly in administration groups （

＜0.05）. SBP， DBP， MAP， LVSP， +LVdp/dt

max

，－LVdp/dt

max

， and ratio of p-PI3K

Tyr607

/PI3K， p-Akt

Thr308

/Akt， p-Akt

ser473

/Akt were increased significantly in

B. balsamifera

total flavonoids medium-dose and high-dose groups （

＜0.05）. The levels of LVEDP， serum myocardial enzymes and inflammatory factors， myocardial infarction rate were all decreased significantly （

＜0.05）.

CONCLUSIONS

B. balsamifera

total flavonoids

can improve cardiac function of AMI model rats， the mechanism of which may be associated with inhibiting the expression of inflammatory factor and activating PI3K/Akt signaling pathway.

OBJECTIVE

To study pharmacodynamics and potential mechanism of

Blumea balsamifera

total flavonoids against acute myocardial infarction （AMI） model rats.

关键词

艾纳香总黄酮急性心肌梗死磷脂酰肌醇3激酶蛋白激酶B心肌保护

Keywords

acute myocardial infarctionphosphoinositide 3-kinaseprotein kinase Bmyocardial protection

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Study on the pharmacodynamics and mechanism of Blumea balsamifera total flavonoids against acute myocardial infarction model rats

DOI：10.6039/j.issn.1001-0408.2023.11.09

摘要

Abstract

关键词

Keywords

references