Screening of active fractions of Jiegu ointment for promoting fracture healing in New Zealand rabbits

ZHENG Guoyao; PENG Lei; XU Yuancui; WU Fei; WU Bo

doi:10.6039/j.issn.1001-0408.2023.11.12

您当前的位置：

首页 >

文章列表页 >

Screening of active fractions of Jiegu ointment for promoting fracture healing in New Zealand rabbits

更新时间：2023-06-09

- Screening of active fractions of Jiegu ointment for promoting fracture healing in New Zealand rabbits
- ZHONGGUO YAOFANG Vol. 34, Issue 11, Pages: 1349-1353(2023)
- 作者机构：
  
  恩施土家族苗族自治州中心医院康复医学中心，湖北恩施　445099
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.11.12
  CLC： R965.1
- Published：15 June 2023，
  
  Received：15 November 2022，
  
  Revised：23 April 2023，
扫描看全文
郑国尧,彭磊,徐元翠等.接骨膏促进新西兰兔骨折愈合的活性部位筛选 ^Δ[J].中国药房,2023,34(11):1349-1353.

ZHENG Guoyao,PENG Lei,XU Yuancui,et al.Screening of active fractions of Jiegu ointment for promoting fracture healing in New Zealand rabbits[J].ZHONGGUO YAOFANG,2023,34(11):1349-1353.
郑国尧,彭磊,徐元翠等.接骨膏促进新西兰兔骨折愈合的活性部位筛选 ^Δ[J].中国药房,2023,34(11):1349-1353. DOI： 10.6039/j.issn.1001-0408.2023.11.12.

ZHENG Guoyao,PENG Lei,XU Yuancui,et al.Screening of active fractions of Jiegu ointment for promoting fracture healing in New Zealand rabbits[J].ZHONGGUO YAOFANG,2023,34(11):1349-1353. DOI： 10.6039/j.issn.1001-0408.2023.11.12.

摘要

目的

筛选接骨膏促进新西兰兔骨折愈合的活性部位。

方法

制备接骨膏醇提物以及乙酸乙酯部位、正丁醇部位，并与炼蜜调制成黏稠度适宜的膏药。以新西兰兔建立左前肢桡骨骨折模型，然后分为模型对照组、醇提物组、乙酸乙酯部位组、正丁醇部位组，每组6只。除模型对照组外，其余各组兔外敷接骨膏相应极性部位膏药，干预4周。通过X线检测兔骨折愈合情况，采用酶联免疫吸附测定法检测兔血清中白细胞介素6（IL-6）、肿瘤坏死因子α（TNF-α）、骨钙素（OC）、血管内皮生长因子A（VEGFA）、碱性成纤维细胞生长因子2（bFGF2）、碱性磷酸酶（ALP）水平，采用苏木素-伊红染色观察兔骨折部位病理学形态变化，采用免疫组化法检测兔骨折部位bFGF2蛋白的表达水平。

结果

正丁醇部位组兔骨折部位的愈合速度最快，醇提物组次之，乙酸乙酯部位组最慢；正丁醇部位组兔血清中TNF-α、IL-6水平下降最快，ALP、bFGF2、OC、VEGFA水平上升最快[且较醇提物组显著升高（

＜0.01）]，骨折部位的软骨细胞完全消失，形成大量骨髓腔，且骨髓腔内骨小梁正式形成，bFGF2表达水平也较醇提物组升高。

结论

接骨膏正丁醇部位促进兔骨折部位愈合的作用强于醇提物以及乙酸乙酯部位，是接骨膏促进骨折愈合的活性部位。

Abstract

OBJECTIVE

To screen the active site of Jiegu ointment in promoting fracture healing in New Zealand rabbits.

METHODS

The ethanol extract of Jiegu ointment， as well as the ethyl acetate and

-butanol parts， were prepared and mixed with honey to form a plaster with appropriate viscosity. The radial fracture model of left forelimb in New Zealand rabbit was established and divided into model control group， ethanol extract group， ethyl acetate fraction group and

-butanol fraction group， with 6 rabbits in each group. Except for model control group， rabbits of all other groups were treated with corresponding polar part of Jiegu ointment for external application， for 4 weeks. The radial fracture healing of rabbits was studied by X-ray examination. Enzyme-linked immunosorbent assay was used to detect the serum levels of interleukin-6 （IL-6）， tumor necrosis factor α （TNF-α）， osteocalcin （OC）， vascular endothelial growth factor A （VEGFA）， basic fibroblast growth factor 2 （bFGF2） and alkaline phosphatase （ALP）. HE staining was adopted to observe the changes of pathological morphology of rabbit fracture site， and immunohistochemical method was used to detect the protein expression of bFGF2 in fracture site of rabbits.

RESULTS

The healing speed of the fracture site in the

-butanol fraction group was the fastest， followed by ethanol extract group， and the ethyl acetate fraction group was the slowest； the serum levels of TNF-α and IL-6 in

-butanol fraction group decreased the fastest， while the levels of ALP， bFGF2， OC and VEGFA increased the fastest [significant increase compared with ethanol extract group （

＜0.01）]； the chondrocytes at the fracture fraction completely disappeared， forming a large number of bone marrow cavities， and the bone trabeculae in the bone marrow cavity were officially formed. The expression level of bFGF2 was also higher than ethanol extract group.

CONCLUSIONS

The effect of

-butanol fraction on promoting fracture healing is more significant than ethyl acetate fraction and ethanol extract， and

-butanol fraction is the active fraction of Jiegu ointment to promote fracture healing.

关键词

接骨膏骨折愈合醇提物正丁醇部位活性部位

Keywords

fracturehealingethanol extractn-butanol fractionactive fraction

references

HUANG H Q，LUO L，LIU Z T，et al. Role of TNF-α and FGF-2 in the fracture healing disorder of type 2 diabetes model induced by high fat diet followed by streptozotocin[J]. Diabetes Metab Syndr Obes，2020，13：2279-2288.

CHEN T H，WEBER F E，MALINA-ALTZINGER J，et al. Epigenetic drugs as new therapy for tumor necrosis factor-α-compromised bone healing[J]. Bone，2019，127：49-58.

胡灏，王任先，万奔，等. 骨发育及损伤修复过程中骨形成与血管形成的偶联作用[J]. 中国骨与关节杂志，2021，10（11）：871-876.

黄幸儒，陈旭，罗天，等. 筋骨疗伤膏外敷对大鼠急性软组织损伤的消肿止痛作用研究[J]. 按摩与康复医学，2021，12（15）：42-46.

YANG S，CHENG J，MAN C，et al. Effects of exogenous nerve growth factor on the expression of BMP-9 and VEGF in the healing of rabbit mandible fracture with local nerve injury[J]. J Orthop Surg Res，2021，16（1）：74.

郭松. 解毒活血化瘀汤对股骨颈骨折术后炎症反应及骨折愈合影响[J]. 中国现代医生，2020，58（1）：73-75.

赵灏，易智. 活血化瘀汤对股骨骨折大鼠骨痂组织胶原表达水平及促进愈合机制分析[J]. 四川中医，2019，37（2）：37-40.

WANG Y J，CHEN W T，ZHAO L，et al. Obesity regulates miR-467/HoxA10 axis on osteogenic differentiation and fracture healing by BMSC-derived exosome LncRNA H19[J]. J Cell Mol Med，2021，25（3）：1712-1724.

ZHANG X Y，CHEN Y P，ZHANG C，et al. Icariin accele- rates fracture healing via activation of the Wnt1/β-catenin osteogenic signaling pathway[J]. Curr Pharm Biotechnol，2020，21（15）：1645-1653.

黄明艳，刘超，王阶. 活血化瘀中药促内皮祖细胞血管修复和新生研究进展[J]. 中国中医药信息杂志，2019，26（7）：141-144.

苗明三，尹俊涛，缪君娴，等.中药外用药理实验存在问题及解决思路[J].时珍国医国药，2008，19（12）：2945-2946.

徐元翠，万仲贤，黄华斌，等. 独正杆不同极性部位促进SD大鼠骨折愈合活性研究[J]. 环球中医药，2018，11（2）：177-181.

魏海峰.染氟成纤维细胞和成骨细胞IGF-1及bFGF的表达及其意义[D].长春：吉林大学，2007.

PAOLI F D，STAELS B，CHINETTI-GBAGUIDI G. Ma- crophage phenotypes and their modulation in atherosclerosis[J]. Circ J，2014，78（8）：1775-1781.

钱洲曜，王勇平. 血管内皮生长因子有望成为加速骨折愈合的全新治疗手段[J]. 中国组织工程研究，2020，24（17）：2759-2769.

段硕，邓丹华，陈磊，等.活血补肾壮骨方对GIOP型大鼠骨折愈合的作用及对骨密度、血清碱性磷酸酶和骨钙素水平的影响[J].中国中医急症，2020，29（10）：1728-1731，1758.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

益智仁不同极性提取物抗老年痴呆作用的研究

酸枣仁镇静催眠活性部位的筛选

线粒体自噬机制、相关疾病及中药对其调节作用的研究进展

Related Author

石绍淮张晨宁刘　冰刘　芷毕开顺贾　英

崔思娇贾英罗洁石绍淮范旭航毕开顺

李凤娇

顾雯

俞捷

董金材

王曦

曾鳞粞

Related Institution

No data

Address：8/F,129 Daping Main Street,Yuzhong District,Chongqing 400042,P.R.China
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰