Study on the extraction and antifungal activity of volatile oil from stems， leaves and roots of <italic style="font-style: italic">Glehnia littoralis</italic>

YANG Zhenmin; LI Bing; MAO Fuying; ZHENG Yuguang; ZHAO Yunsheng

doi:10.6039/j.issn.1001-0408.2023.11.14

您当前的位置：

首页 >

文章列表页 >

Study on the extraction and antifungal activity of volatile oil from stems， leaves and roots of Glehnia littoralis

更新时间：2023-06-09

- Study on the extraction and antifungal activity of volatile oil from stems， leaves and roots of Glehnia littoralis
- ZHONGGUO YAOFANG Vol. 34, Issue 11, Pages: 1358-1362(2023)
- 作者机构：
  
  1.河北中医学院药学院，石家庄　050200
  2.河北中医学院河北省中药炮制技术创新中心，石家庄　050091
  3.河北省中药资源利用与质量评价国际联合研究中心，石家庄　050091
  4.河北中医学院实验中心，石家庄　050200
  5.河北化工医药职业技术学院，石家庄　050026
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.11.14
  CLC： R284;R965
- Published：15 June 2023，
  
  Received：12 January 2023，
  
  Revised：02 April 2023，
扫描看全文
杨震旻,李冰,毛福英等.北沙参茎叶和根中挥发油的提取与抗真菌活性研究 ^Δ[J].中国药房,2023,34(11):1358-1362.

YANG Zhenmin,LI Bing,MAO Fuying,et al.Study on the extraction and antifungal activity of volatile oil from stems， leaves and roots of Glehnia littoralis[J].ZHONGGUO YAOFANG,2023,34(11):1358-1362.
杨震旻,李冰,毛福英等.北沙参茎叶和根中挥发油的提取与抗真菌活性研究 ^Δ[J].中国药房,2023,34(11):1358-1362. DOI： 10.6039/j.issn.1001-0408.2023.11.14.

YANG Zhenmin,LI Bing,MAO Fuying,et al.Study on the extraction and antifungal activity of volatile oil from stems， leaves and roots of Glehnia littoralis[J].ZHONGGUO YAOFANG,2023,34(11):1358-1362. DOI： 10.6039/j.issn.1001-0408.2023.11.14.

摘要

目的

探究北沙参茎叶和根中挥发油的提取工艺，解析北沙参茎叶和根中挥发油所含化学成分，并初步评价其体外抗真菌活性。

方法

以水蒸气蒸馏法为基础，通过单因素与正交实验优化北沙参茎叶和根中挥发油提取方法，利用气相色谱-质谱联用（GC-MS）技术鉴定北沙参茎叶和根中挥发油所含化学成分并计算其相对百分含量。采用纸片扩散法测定北沙参茎叶和根中挥发油对茄病镰刀菌、半裸镰刀菌、尖孢镰刀菌、寄生曲霉和黄曲霉的抗真菌活性。

结果

北沙参最佳提取工艺为料液比1∶15，蒸馏时间5 h，KCl浓度15%。从北沙参茎叶挥发油中鉴定出11种成分，根挥发油中共鉴定出8种成分。人参炔醇为北沙参茎叶挥发油和根挥发油的共有成分，其在茎叶中的含量为38.21%，根中的含量为74.02%。北沙参茎叶挥发油和根挥发油对茄病镰刀菌、半裸镰刀菌、尖孢镰刀菌、寄生曲霉和黄曲霉均有一定的抑制作用，以茎叶挥发油较强。

结论

北沙参茎叶挥发油和根挥发油的化学成分差异明显，但二者均具有一定的体外抗真菌活性。

Abstract

OBJECTIVE

To explore the extraction process of volatile oil from the stems， leaves and roots of

Glehnia littoralis

， analyze the chemical components of the volatile oil from the stems， leaves and roots of

G. littoralis

， and preliminarily evaluate its

in vitro

antifungal activity.

METHODS

Based on the steam distillation method， single factor test and orthogonal experiment were conducted to optimize the extraction method of volatile oil from the stems， leaves and roots of

G. littoralis

. The chemical components of the volatile oil from the stems， leaves and roots of

G. littoralis

were identified by using gas chromatography-mass spectrometry （GC-MS) technology and their relative contents were calculated. The antifungal activity of volatile oils from the stems， leaves and roots of

G. littoralis

against

Fusarium solani， Fusarium incarnatum， Fusarium oxysporum， Aspergillus parasiticus

and

Aspergillus flavus

was determined by paper diffusion method.

RESULTS

The optimal extraction process of

G. littoralis

was solid-liquid ratio of 1∶15， distillation time of 5 hours， and KCl concentration of 15%. Eleven components were identified from the volatile oil of the stems and leaves of

G. littoralis

， and a total of eight components were identified from the volatile oil of the roots. Ginsenethinol was a common component in the volatile oil from the stems， leaves and roots of

G. littoralis

， its contents in the stems and leaves， roots were 38.21% and 74.02%， respectively. The volatile oil from the stems， leaves and roots of

G. littoralis

had a certain inhibitory effect on

F. solani， F. incarnatum， F. oxysporum， A. parasiticus and A. flavus

， especially volatile oil from the stems and leaves.

CONCLUSIONS

There is a significant difference in chemical components of the volatile oil between the roots， stems and leaves of

G. littoralis

， both of which have certain

in vitro

antifungal activity.

关键词

北沙参茎叶根挥发油真菌抗真菌活性化学成分

Keywords

stemsleavesrootsvolatile oilfungusantifungal activitychemical components

references

YUAN Z，TEZUKA Y，FAN W Z，et al. Constituents of the underground parts of Glehnia littoralis[J]. Chem Pharm Bull （Tokyo），2002，50（1）：73-77.

国家药典委员会.中华人民共和国药典：四部[S]. 2020年版.北京：中国医药科技出版社，2020：103-104.

PAN Y X，CHU J M，YANG H X. Conservation choice on the rare endangered plants Glehnia littoralis[J]. Conserv Physiol，2018，6（1）：coy002.

环境保护部，中国科学院.关于发布《中国生物多样性红色名录-高等植物卷》的公告[EB/OL].（2013-09-02）[2023-01-11]. https：//www.mee.gov.cn/gkml/hbb/bgg/201309/W020130917614244055331.pdfhttps://www.mee.gov.cn/gkml/hbb/bgg/201309/W020130917614244055331.pdf.

YOON T，LEE D Y，LEE A Y，et al. Anti-inflammatory effects of Glehnia littoralis extract in acute and chronic cutaneous inflammation[J]. Immunopharmacol Immunotoxicol，2010，32（4）：663-670.

UM Y R，KONG C S，LEE J I，et al . Evaluation of chemical constituents from Glehnia littoralis for antiproliferative activity against HT-29 human colon cancer cells[J]. Process Biochem，2010，45（1）：114-119.

HONG H，DELA CRUZ J F，KIM W S，et al. Glehnia littoralis root extract inhibits fat accumulation in 3T3-L1 cells and high-fat diet-induced obese mice by downregulating adipogenic gene expression[J]. Evid Based Complement Alternat Med，2018，2018：1243049.

邱晓月，景永帅，郑玉光，等. 北沙参多糖对免疫系统调节作用研究进展[J]. 中国药理学与毒理学杂志，2021，35（10）：794.

侯晓强，任秀艳，付亚娟，等. 北沙参内生真菌的抑菌活性与分类研究[J]. 中草药，2015，46（19）：2932-2936.

吕方军，叶国华，许一平，等. 北沙参茎叶多糖的含量测定[J]. 中药新药与临床药理，2012，23（1）：84-86.

杨同章，叶国华，许一平，等. 北沙参茎叶黄酮类化合物提取优选条件研究[J]. 中药新药与临床药理，2014，25（1）：85-88.

冯子晋，卢小玲，张建鹏，等. 北沙参中香豆素类与聚炔类成分的含量测定研究[J]. 中国海洋药物，2014，33（3）：20-26.

许一平，梁伟玲，叶国华，等. ICP-MS同时测定北沙参茎叶中14种元素[J]. 化工管理，2021（17）：57-58，61.

王辉明，吕志强，张颢儿，等. 不同产地北沙参药材的挥发油成分比较分析[J]. 中国药房，2019，30（22）：3109-3112.

FILLY A，FABIANO-TIXIER A S，LOUIS C，et al. Water as a green solvent combined with different techniques for extraction of essential oil from lavender flowers[J]. Comptes Rendus Chimie，2016，19（6）：707-717.

张建云，赵艳云，赵云生，等.北苍术药用和非药用部位挥发油成分分析及体外抗菌活性考察[J].中国药房，2022，33（21）：2609-2614.

KOPRIVNJAK O，BRKIĆ BUBOLA K，KOSIĆ U. Sodium chloride compared to talc as processing aid has similar impact on volatile compounds but more favorable on ortho-diphenols in virgin olive oil[J]. Eur J Lipid Sci Technol，2016，118（2）：318-324.

FU C，LAN X，YUAN J，et al. Research on the optimization，key chemical constituents and antibacterial activity of the essential oil extraction process of Thuja koraiensis Nakai[J]. J Microbiol Methods，2022，194：106435.

张宁慧，王瑱，毛垣元，等. 植物精油及活性成分防控黄曲霉生长及其毒素产生的研究进展[J]. 中草药，2020，51（20）：5348-5359.

林镇跃，阙友雄，刘平武，等. 植物致病镰刀菌的研究进展[J]. 中国糖料，2014，36（1）：58-64，78.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

野马追的化学成分与药理作用研究进展

生藤乙酸乙酯部位的化学成分研究

三棱化学成分与质量评价的研究进展

泻下类中药性味归经与化学成分的相关性

独子藤茎的化学成分研究

Related Author

吴双庆夏龙姚士韩海燕张健

莫小宇麦景标

王新胜吴启南陈广云沈蓓贺潇潇伍城颖

林声在余斌张勉

陈铭祥喻文进林晓李尚德王定勇

李文静洪博赵春杰

孙晓业吴红华张鹏

孙玉婉汪春牛韩星刘平怀

Related Institution

No data

Address：8/F,129 Daping Main Street,Yuzhong District,Chongqing 400042,P.R.China
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Study on the extraction and antifungal activity of volatile oil from stems， leaves and roots of Glehnia littoralis

DOI：10.6039/j.issn.1001-0408.2023.11.14

摘要

Abstract

关键词

Keywords

references