Material basis and mechanism of Kazakh classic prescription Wuzdekh in the treatment of enteritis

JIA Liping; CHENG Bo; SHI Yuzhu; HE Jiang; WANG Xue; YANG Weijun

doi:10.6039/j.issn.1001-0408.2023.13.07

您当前的位置：

首页 >

文章列表页 >

Material basis and mechanism of Kazakh classic prescription Wuzdekh in the treatment of enteritis

更新时间：2023-08-15

- Material basis and mechanism of Kazakh classic prescription Wuzdekh in the treatment of enteritis
- ZHONGGUO YAOFANG Vol. 34, Issue 13, Pages: 1577-1583(2023)
- 作者机构：
  
  1.新疆农业大学食品科学与药学学院，乌鲁木齐　830004
  2.新疆维吾尔自治区药物研究所新疆维吾尔药重点实验室，乌鲁木齐　830004
  3.新疆维吾尔自治区药物研究所药理Ⅱ实验室，乌鲁木齐　830004
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.13.07
  CLC： R965
- Published：15 July 2023，
  
  Received：29 January 2023，
  
  Revised：16 March 2023，
扫描看全文
贾丽萍,程波,史玉柱等.哈萨克医经典名方吾孜德克治疗肠炎的物质基础及作用机制 ^Δ[J].中国药房,2023,34(13):1577-1583.

JIA Liping,CHENG Bo,SHI Yuzhu,et al.Material basis and mechanism of Kazakh classic prescription Wuzdekh in the treatment of enteritis[J].ZHONGGUO YAOFANG,2023,34(13):1577-1583.
贾丽萍,程波,史玉柱等.哈萨克医经典名方吾孜德克治疗肠炎的物质基础及作用机制 ^Δ[J].中国药房,2023,34(13):1577-1583. DOI： 10.6039/j.issn.1001-0408.2023.13.07.

JIA Liping,CHENG Bo,SHI Yuzhu,et al.Material basis and mechanism of Kazakh classic prescription Wuzdekh in the treatment of enteritis[J].ZHONGGUO YAOFANG,2023,34(13):1577-1583. DOI： 10.6039/j.issn.1001-0408.2023.13.07.

摘要

目的

探讨哈萨克医经典名方吾孜德克（WZDK）治疗肠炎的物质基础及潜在作用机制。

方法

利用液相色谱-串联质谱（LC-MS/MS）技术分析WZDK中的化学成分。通过网络药理学和分子对接技术筛选WZDK的主要化学成分并进行靶点预测，通过体内实验验证WZDK对急性肠炎的治疗作用及作用靶点。采用葡聚糖硫酸钠诱导构建小鼠急性肠炎模型，给药期间观察小鼠一般状况并计算疾病活动指数（DAI）评分，通过苏木素-伊红染色、实时荧光定量PCR验证各组小鼠肠道病理改变及核心靶点mRNA的表达。

结果

通过LC-MS/MS技术分析共得到316种化学成分；通过网络药理学分析，核心靶点主要包括白细胞介素1β（IL-1β）、蛋白激酶B1（AKT1）、肿瘤蛋白p53（TP53）、IL-6、肿瘤坏死因子（TNF）等；分子对接结果显示，白桦脂酸、lappadilactone、木香烃内酯、去氢木香内酯等化学成分与核心靶点结合稳定。体内实验结果显示，与模型组比较，高剂量（5.00 g/kg）WZDK能显著降低小鼠的DAI评分（

＜0.05），改善其结肠组织炎症细胞浸润、黏膜组织损伤，显著下调其结肠组织中IL-6、TNF-α、IL-1β和TP53 mRNA的表达（

＜0.05或

＜0.01）。

结论

WZDK中的白桦脂酸、lappadilactone、木香烃内酯、去氢木香内酯等多种化学成分可能通过下调结肠组织中TNF-α、IL-6、IL-1β、TP53 mRNA表达，发挥改善肠道局部炎症因子失衡、修复结肠黏膜组织损伤的作用。

Abstract

OBJECTIVE

To explore the material basis and potential mechanism of Kazakh classic prescription Wuzdekh （WZDK） in the treatment of enteritis.

METHODS

LC-MS/MS technology was used to analyze the chemical components in WZDK. Through network pharmacology and molecular docking technology， the main chemical components of WZDK were screened and the target was predicted； therapeutic effect and target of WZDK on acute enteritis were verified through

in vivo

experiments. The acute enteritis model of mice was induced by dextran sulfate sodium salt； the general condition of the mice was observed during administration and the disease activity index （DAI） score was calculated； pathological changes of the intestine and mRNA expression of core target were validated by HE staining and quantitative real-time PCR.

RESULTS

A total of 316 chemical components were obtained by LC-MS/MS. The core targets of network pharmacological analysis mainly included interleukin 1β（IL-1β）， protein kinase B1 （AKT1）， tumor protein p53 （TP53）， IL-6， tumor necrosis factor （TNF） and so on. The results of molecular docking showed that chemical components such as mairin， lappadilactone， costunolide and dehydrocostus lactone were stable in binding to the core target. The results of

in vivo

experiment showed that， compared with model group， high dose （5.00 g/kg） of WZDK could significantly reduce the DAI score （

＜0.05）， improve inflammatory cell infiltration and mucosal tissue damage of colon tissue， and significantly down-regulated mRNA expressions of IL-6， TNF-α， IL-1β and TP53 in colon tissue（

＜0.05 or

＜0.01）.

CONCLUSIONS

Chemical components of WZDK such as mairin， lappadilactone， costunolide and dehydrocostus lactone may play the role of improving the imbalance of local inflammatory factors in the intestine and repairing damage of colonic mucosal tissue by down-regulating mRNA expressions of TNF-α， IL-6， IL-1β and TP53 in colon tissue.

关键词

吾孜德克哈萨克医经典名方网络药理学分子对接肠炎

Keywords

Kazakh medicineclassic prescriptionnetwork pharmacologymolecular dockingenteritis

references

KUMAZAKI M，USUKU S. Epidemiological and genetic analysis of human group C rotaviruses isolated from outbreaks of acute gastroenteritis in Yokohama，Japan，between 2006 and 2012[J]. Arch Virol，2014，159（4）：761-771.

KAPLAN G G. The global burden of IBD：from 2015 to 2025[J]. Nat Rev Gastroenterol Hepatol，2015，12（12）：720-727.

AHMAD H，KUMAR V L. Pharmacotherapy of ulcera- tive colitis-current status and emerging trends[J]. J Basic Clin Physiol Pharmacol，2018，29（6）：581-592.

肖四方，马小华，石国民，等. 抗痨颗粒治疗结核病分子机制的网络药理和实验验证[J]. 中国实验方剂学杂志，2022，28（4）：205-211.

杜世拔，李国政，郑靓，等.基于网络药理学的新加白虎汤治疗痛风性关节炎的作用机制及抗炎作用初步验证[J].药物评价研究，2022，45（2）：266-273.

乌太波依达克.医药志[M].乌鲁木齐：新疆科技卫生出版社，1994：346.

中央民族大学民族药医院制剂目录课题组. 中国民族药医院制剂目录：第四卷[M]. 北京：化学工业出版社，2021：114.

余秋香，李思雨，常安，等. 消食颗粒化学成分鉴定及其治疗功能性消化不良的潜在作用机制研究[J]. 中国药房，2022，33（23）：2874-2879.

龚普阳，郭瑜婕，李晓朋，等. 基于网络药理学与分子对接技术的金花清感颗粒防治新型冠状病毒肺炎的潜在药效物质研究[J]. 中草药，2020，51（7）：1685-1693.

王开，裴志花，胡桂学，等.肠炎动物模型的研究进展[J].中国免疫学杂志，2015，31（5）：704-706.

CAO H Y，LIU J X，SHEN P，et al. Protective effect of naringin on DSS-induced ulcerative colitis in mice[J]. J Agric Food Chem，2018，66（50）：13133-13140.

宋洁，郭瑞芳，聂虹，等. 木香烃内酯对溃疡性结肠炎小鼠肠道免疫炎症的影响及其机制[J]. 广西医科大学学报，2021，38（12）：2300-2305.

陈媛媛. 去氢木香内酯对炎症小体的调控作用及机制研究[D]. 济南：山东中医药大学，2020.

田颖颖，李依林，田时秋，等.去氢木香内酯激活凋亡与自噬抑制人肺癌A549细胞生长[J].中国实验方剂学杂志，2023，29（2）：73-80.

黄飘. 白桦脂酸抑制Wnt通路和抗大肠癌作用的分子机制研究[D].上海：上海中医药大学，2020.

郑加梅，尚明越，王嘉乐，等. 木香的化学成分、药理作用、临床应用研究进展及质量标志物预测[J]. 中草药，2022，53（13）：4198-4213.

王宏伟，刘冰晶，杜晓鹃，等. 拳参乙酸乙酯萃取部位的化学成分鉴定[J]. 中国药房，2017，28（25）：3494-3497.

崔健，赵奎君. 基于生物热动力学的拳参对大肠埃希菌抑制作用[J]. 中国实验方剂学杂志，2018，24（12）：57-61.

李纬博. 白屈菜生物活性化学成分的研究[D]. 咸阳：西北农林科技大学，2015.

徐香琴，黄松，杜先华，等. 白屈菜红碱对小鼠溃疡性结肠炎的治疗作用[J]. 中国实验方剂学杂志，2014，20（21）：171-174.

张志宏. 白屈菜碱对NF-κB活化及相应靶基因表达的影响[D]. 延吉：延边大学，2018.

GORETSKY T，DIRISINA R，SINH P，et al. p53 mediates TNF-induced epithelial cell apoptosis in IBD[J]. Am J Pathol，2012，181（4）：1306-1315.

胡万祥. 丝胶蛋白靶向Akt1调控PI3K/Akt/NF-κB信号通路保护STZ致损伤INS-1细胞的机制研究[D]. 承德：承德医学院，2022 .

DU L J，KIM J J，SHEN J H，et al. KRAS and TP53 mutations in inflammatory bowel disease-associated colorectal cancer：a meta-analysis[J]. Oncotarget，2017，8（13）：22175-22186.

UNVER N，MCALLISTER F. IL-6 family cytokines：key inflammatory mediators as biomarkers and potential therapeutic targets[J]. Cytokine Growth Factor Rev，2018，41：10-17.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on the effects and mechanism of luteolin on osteogenic repair of bone defects

Identification of the chemical composition of Xiaoshi granules and study on its potential mechanism of the treatment of functional dyspepsia

Related Author

TANG Shengyao

HU Minhua

ZHOU Ruoyu

SUN Weipeng

TANG Xintao

LIN Haixiong

JIANG Ziwei

YU Qiuxiang

Related Institution

The First Clinical Medical College， Guangzhou University of Chinese Medicine

Ningxia Hui Autonomous Region Hospital of Traditional Chinese Medicine&Academy of Traditional Chinese Medicine

Institute of Tissue Engineering and Regenerative Medicine， the Chinese University of Hong Kong

Dept. One of Orthopedics， the First Affiliated Hospital of Guangzhou University of Chinese Medicine

School of Pharmacy， Liaoning University of Traditional Chinese Medicine

Address：8/F,129 Daping Main Street,Yuzhong District,Chongqing 400042,P.R.China
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰