Study on the intestinal absorption characteristics of saikosaponins

WANG Yazhi; WANG Qiyi; FENG Wenzhong; CHEN Shuangshuang; SUN Xinguang; ZHOU Lijuan; ZHANG Yan; ZHANG Jianyong; DUAN Cancan

doi:10.6039/j.issn.1001-0408.2023.14.04

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Study on the intestinal absorption characteristics of saikosaponins

更新时间：2023-07-24

- Study on the intestinal absorption characteristics of saikosaponins
- ZHONGGUO YAOFANG Vol. 34, Issue 14, Pages: 1681-1685(2023)
- 作者机构：
  
  1.遵义医科大学药学院，贵州遵义　563000
  2.北京振东光明药物研究院有限公司，北京　100085
  3.遵义医科大学基础药理教育部重点实验室暨特色民族药教育部国际合作联合实验室，贵州遵义　563000
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.14.04
  CLC： R969.1
- Published：30 July 2023，
  
  Received：16 December 2022，
  
  Revised：17 May 2023，
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王雅芝,王启益,冯文忠等.柴胡皂苷类成分的肠吸收特性研究 ^Δ[J].中国药房,2023,34(14):1681-1685.

WANG Yazhi,WANG Qiyi,FENG Wenzhong,et al.Study on the intestinal absorption characteristics of saikosaponins[J].ZHONGGUO YAOFANG,2023,34(14):1681-1685.
王雅芝,王启益,冯文忠等.柴胡皂苷类成分的肠吸收特性研究 ^Δ[J].中国药房,2023,34(14):1681-1685. DOI： 10.6039/j.issn.1001-0408.2023.14.04.

WANG Yazhi,WANG Qiyi,FENG Wenzhong,et al.Study on the intestinal absorption characteristics of saikosaponins[J].ZHONGGUO YAOFANG,2023,34(14):1681-1685. DOI： 10.6039/j.issn.1001-0408.2023.14.04.

摘要

目的

探讨柴胡皂苷类成分的肠吸收特性。

方法

基于大鼠外翻肠囊模型，以累积吸收量（

）、吸收速率常数（

）为指标，以超高效液相色谱-串联质谱技术为手段，检测柴胡总皂苷提取液（8 g/mL，以生药量计）中柴胡皂苷A、B

、C、D、F在十二指肠、空肠、回肠中的吸收情况。

结果

柴胡皂苷A、B

、C、F在十二指肠、空肠、回肠中吸收回归方程的相关系数（

）均大于0.95，柴胡皂苷D在上述肠段中的

均小于0.95；与同成分在十二指肠中的吸收比较，柴胡皂苷A、C在空肠、回肠中循环120 min时的

、

以及柴胡皂苷F在回肠中循环120 min时的

、

均显著降低（

＜0.05）。

结论

柴胡皂苷A等5种柴胡皂苷类成分在十二指肠、空肠、回肠中均有吸收，其中柴胡皂苷A、B

、C、F均为线性吸收，符合零级吸收特征；柴胡皂苷D为非线性吸收。

Abstract

OBJECTIVE

To explore the intestinal absorption characteristics of saikosaponins.

METHODS

Based on everted intestinal sac model， using accumulative absorption amount （

） and absorption rate constant （

） as indexes， UHPLC-MS/MS technique as a method， the absorption of saikosaponin A， B

， C， D and F from total saponins of

Bupleurum chinense

（8 g/mL， by crude drug） in the duodenum， jejunum and ileum was detected.

RESULTS

The correlation coefficients （

） of the regression equations for the absorption of saikosaponins A， B

， C and F in the duodenum， jejunum and ileum were all higher than 0.95， while the

of saikosaponin D in the above intestinal segments was lower than 0.95； compared with the absorption of the same composition in the duodenum， the

and

of saikosaponin A and C circulating in jejunum and ileum for 120 min， as well as the

and

of saikosaponin F circulating in the ileum for 120 min were significantly decreased （

＜0.05）.

CONCLUSIONS

Saikosaponin A and the other 4 saikosaponins are all absorbed in the duodenum， jejunum and ileum； among them， saikosaponin A， B

， C and F are linearly absorbed， which conforms to the zero-order absorption characteristics， but saikosaponin D shows non-linear absorption.

关键词

柴胡皂苷类成分外翻肠囊法累积吸收量吸收速率常数超高效液相色谱-串联质谱技术

Keywords

saikosaponinseverted intestinal sac experimentaccumulative absorption amountabsorption rate constantUHPLC-MS/MS

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