浏览全部资源
扫码关注微信
- Journal Information
- Archive
- Submission
- For Reviewers
- Statement and Policies
Influence of Huayu xiaozhong decoction on inflammatory response in rats with deep vein thrombosis
- ZHONGGUO YAOFANG Vol. 34, Issue 14, Pages: 1697-1702(2023)
- 作者机构：
海南省中医院重症医学科，海口 570203
- 作者简介：
- 基金信息：
DOI：10.6039/j.issn.1001-0408.2023.14.07
CLC： R965;R543.6Published：30 July 2023，
Received：12 December 2022，
Revised：19 May 2023，
扫 描 看 全 文
麦叶,林道斌,刘海林等.化瘀消肿汤对深静脉血栓形成大鼠炎症反应的影响 Δ[J].中国药房,2023,34(14):1697-1702.
MAI Ye,LIN Daobin,LIU Hailin,et al.Influence of Huayu xiaozhong decoction on inflammatory response in rats with deep vein thrombosis[J].ZHONGGUO YAOFANG,2023,34(14):1697-1702.
麦叶,林道斌,刘海林等.化瘀消肿汤对深静脉血栓形成大鼠炎症反应的影响 Δ[J].中国药房,2023,34(14):1697-1702. DOI： 10.6039/j.issn.1001-0408.2023.14.07.
MAI Ye,LIN Daobin,LIU Hailin,et al.Influence of Huayu xiaozhong decoction on inflammatory response in rats with deep vein thrombosis[J].ZHONGGUO YAOFANG,2023,34(14):1697-1702. DOI： 10.6039/j.issn.1001-0408.2023.14.07.
摘要
目的
2
探讨化瘀消肿汤（HXD）对深静脉血栓形成（DVT）大鼠炎症反应的影响。
方法
2
将雄性SD大鼠分为对照组（CK组）、模型组（Model组）、HXD低剂量组（HXD-L组，HXD 10.86 mg/kg）、HXD中剂量组（HXD-M组，HXD 21.71 mg/kg）、HXD高剂量组（HXD-H组，HXD 32.57 mg/kg）、阳性对照组（LMWHS组，低分子量肝素钠600 IU/kg）、沉默信息调节因子2（SIRT2）抑制剂组（AK-7组，AK-7 20 mg/kg）、HXD-M联合AK-7组（HXD-M+AK-7组，HXD 21.71 mg/kg+AK-7 20 mg/kg），每组12只。除CK组外，其余组大鼠均采用Reyers法构建DVT大鼠模型；建模后，各药物组分别灌胃/腹腔注射相应药液，每天1次，持续2周。末次给药24 h后，检测大鼠凝血功能指标[活化部分凝血活酶时间（APTT）、凝血酶时间（TT）、凝血酶原时间（PT）、纤维蛋白原（FIB）]和血清、下腔静脉组织中炎症指标[白细胞介素1β（IL-1β）、IL-6、肿瘤坏死因子α（TNF-α）]，观察其血栓形成情况并称定血栓湿重、干重，检测下腔静脉组织中组织因子（TF）、SIRT2蛋白的表达情况和核因子κB（NF-κB）p65的磷酸化、乙酰化水平。
结果
2
与CK组比较，Model组大鼠的APTT、TT、PT均显著缩短（
P
＜0.05）；FIB含量，IL-1β、IL-6、TNF-α水平，静脉血栓湿重、干重，TF蛋白染色评分，NF-κB p65蛋白的磷酸化、乙酰化水平均显著升高（
P
＜0.05）；下腔静脉血管内充满血栓，SIRT2蛋白的表达水平显著降低（
P
＜0.05）。与Model组比较，HXD-L组、HXD-M组、HXD-H组和LMWHS组大鼠上述指标均显著改善，且HXD-M组、HXD-H和LMWHS组的效果显著优于HXD-L组（
P
＜0.05）；AK-7组大鼠上述指标的变化趋势与之相反，且AK-7可减弱中剂量HXD对模型大鼠炎症反应的抑制作用（
P
＜0.05）。
结论
2
HXD可能通过激活SIRT2/NF-κB信号通路来抑制DVT大鼠的炎症反应。
Abstract
OBJECTIVE
2
To investigate the influence of Huayu xiaozhong decoction （HXD） on inflammatory response in rats with deep vein thrombosis （DVT）.
METHODS
2
The male SD rats were divided into control group （CK group）， model group （Model group）， HXD low-dose group （HXD-L group， HXD 10.86 mg/kg）， HXD medium-dose group （HXD-M group， HXD 21.71 mg/kg）， HXD high-dose group （HXD-H group， HXD 32.57 mg/kg）， positive control group （LMWHS group， low molecular weight heparin sodium 600 IU/kg）， silent information regulator 2 （SIRT2） inhibitor group （AK-7 group， AK-7 20 mg/kg）， HXD-M+AK-7 group （HXD 21.71 mg/kg+AK-7 20 mg/kg）， with 12 rats in each group. Except for the CK group， the DVT rat was induced by the Reyers method in other groups； after modeling， administration groups were given relevant medicine intragastrically/intraperitoneally， once a day， for consecutive 2 weeks. Twenty-four hours after the last medication， the coagulation function indexes [activated partial thromboplastin time （APTT）， thrombin time （TT）， prothrombin time （PT）， fibrinogen （FIB）] and inflammatory indexes in serum and inferior vena cava tissue [interleukin-1β （IL-1β）， IL-6， tumor necrosis factor-α （TNF-α）] of rats were detected. The formation of thrombus was observed， and the wet and dry masses of the thrombus were weighed. The protein expressions of tissue factor （TF） and SIRT2 as well as the phosphorylation and acetylation levels of nuclear factor kappa B （NF-κB） p65 in inferior vena cava tissue were detected.
RESULTS
2
Compared with CK group， APTT， TT and PT of rats in Model group were shortened significantly（
P
＜0.05）； the content of FIB， the levels of IL-1β， IL-6 and TNF-α， wet weight and dry weight of venous thrombus， TF protein staining score， the phosphorylation and acetylation levels of NF-κB p65 protein increased significantly （
P
＜0.05）； the inferior vena cava was full of thrombus， and the protein expression of SIRT2 decreased （
P
＜0.05）. Compared with Model group， above indexes of HXD-L group， HXD-M group， HXD-H group and LMWHS group were improved， while the improvement effects of HXD-M group， HXD-H group and LMWHS group were significantly better than those of HXD-L group （
P
＜0.05）. The trends of the corresponding indicators in AK-7 group were opposite to the above （
P
＜0.05）； AK-7 attenuated the inhibitory effect of medium-dose HXD on the inflammatory response in model rats （
P
＜0.05）.
CONCLUSIONS
2
HXD may inhibit the inflammatory response of DVT rats by activating SIRT2/NF-κB signaling pathway.
关键词
化瘀消肿汤深静脉血栓形成炎症反应沉默信息调节因子2/核因子κB信号通路
Keywords
deep vein thrombosisinflammatory responsesilent information regulator 2/nuclear transcription factor kappa B signaling pathway
references
YANG B C，ZHANG Z X. Suppression of long intergenic non-protein coding RNA 1123 constrains lower extremity deep vein thrombosis via microRNA-125a-3p to target interleukin 1 receptor type 1[J]. Bioengineered，2022，13（5）：13452-13461.
JIN F M，WANG M，WU X M，et al. Effects of wasp venom on venous thrombosis in rats[J]. Iran J Basic Med Sci，2022，25（7）：822-826.
ZHANG T S，LI Q，WANG L J，et al. Expression va-riations and clinical significance of MMP-1，MMP-2 and inflammatory factors in serum of patients with deep venous thrombosis of lower extremity[J]. Exp Ther Med，2019，17（1）：181-186.
宋晓东，于彬. 化瘀消肿汤预防人工股骨头置换术后下肢深静脉血栓形成（DVT）的疗效及凝血功能的对比研究[J]. 中国伤残医学，2021，29（9）：16-17.
YAO X L，CHEN W P，LIU J，et al. Deep vein thrombosis is modulated by inflammation regulated via sirtuin 1/NF-κB signalling pathway in a rat model[J]. Thromb Haemost，2019，119（3）：421-430.
DEMYANENKO S，GANTSGORN E，RODKIN S，et al. Localization and expression of sirtuins 1，2，6 and plasticity-related proteins in the recovery period after a photothrombotic stroke in mice[J]. J Stroke Cerebrovasc Dis，2020，29（10）：105152.
刘青云，黄振华，刘亚坤，等. 乳脂球表皮生长因子8通过调控Toll样因子受体4/核因子-κB信号通路抑制深静脉血栓形成[J]. 中华实验外科杂志，2022，39（2）：250-253.
陈美雄，林业武，周理，等. 化瘀消肿汤预防人工股骨头置换术后下肢深静脉血栓形成的围手术期临床效能[J]. 广州中医药大学学报，2015，32（6）：1011-1016.
谢义松，袁万福，刘晓岚，等. 血府逐瘀汤对大鼠深静脉血栓预防作用的实验研究[J]. 湖南中医杂志，2019，35（9）：137-139.
YANG L，YU X F，ZHANG Y J，et al. Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic-ischemic white matter damage[J]. CNS Neurosci Ther，2022，28（7）：1019-1032.
兴成娟，陈素贤，李伦，等. 结直肠癌中HMGA2表达的意义与微卫星不稳定性的关系[J]. 中国老年学杂志，2019，39（2）：285-289.
ZHANG Y H，ZHANG Z，WEI R，et al. IL（interleukin）-6 contributes to deep vein thrombosis and is negatively regulated by miR-338-5p[J]. Arterioscler Thromb Vasc Biol，2020，40（2）：323-334.
ZENG S，YI R K，TAN F，et al. Lactobacillus plantarum HFY05 attenuates carrageenan-induced thrombosis in mice by regulating NF-κB pathway-associated inflammatory responses[J]. Front Nutr，2022，9：813899.
么秀洁，赵志梅，夏天.炎症与血栓形成[J].血栓与止血学，2015，21（3）：190-192.
HANTRAKOOL S，KUMFU S，CHATTIPAKORN S C，et al. Effects of particulate matter on inflammation and thrombosis：past evidence for future prevention[J]. Int J Environ Res Public Health，2022，19（14）：8771.
JIN Z X，GAO L，ZHANG L，et al. Antimicrobial activity of saponins produced by two novel endophytic fungi from Panax notoginseng[J]. Nat Prod Res，2017，31（22）：2700-2703.
ZHANG Z Q，SONG J Y，JIA Y Q，et al. Buyanghuanwu decoction promotes angiogenesis after cerebral ischemia/reperfusion injury：mechanisms of brain tissue repair[J]. Neural Regen Res，2016，11（3）：435-440.
ZHU M，TANG Y P，DUAN J A，et al. Roles of paeoniflorin and senkyunolide Ⅰ in SiWu decoction on antiplatelet and anticoagulation activities[J]. J Sep Sci，2010，33（21）：3335-3340.
WU Z Y，LI H，TANG Y J. Effect of simvastatin on the SIRT2/NF-κB pathway in rats with acute pulmonary embolism[J]. Pharm Biol，2018，56（1）：511-518.
YUAN F，XU Z M，LU L Y，et al. SIRT2 inhibition exa-cerbates neuroinflammation and blood-brain barrier disruption in experimental traumatic brain injury by enhancing NF-κB p65 acetylation and activation[J]. J Neurochem，2016，136（3）：581-593.
0
Views
2
下载量
0
CSCD
- L-PDFDownload
- WORDDownload
- Meta-XMLDownload
- BibTeXDownload
- EndnoteDownload
- RefMan Download
- NoteExpressDownload
- NoteFirstDownload
Publicity Resources
Related Articles
Related Author
Related Institution
- Address：8/F,129 Daping Main Street,Yuzhong District,Chongqing 400042,P.R.China
- Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号
公网安备50010302001817 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.