Establishment of quantitative analysis of multi-components by single marker for content determination of 10 ganoderic acids in <italic style="font-style: italic">Ganoderma lucidum</italic>

LUO Shu; SONG Yi; LUO Xia; JIANG Nan; YU Mengyao; XU Xiaoyan

doi:10.6039/j.issn.1001-0408.2023.14.08

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Establishment of quantitative analysis of multi-components by single marker for content determination of 10 ganoderic acids in Ganoderma lucidum

更新时间：2023-07-24

- Establishment of quantitative analysis of multi-components by single marker for content determination of 10 ganoderic acids in Ganoderma lucidum
- ZHONGGUO YAOFANG Vol. 34, Issue 14, Pages: 1703-1706(2023)
- 作者机构：
  
  四川省中医药科学院，成都　610041
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.14.08
  CLC： R917
- Published：30 July 2023，
  
  Received：18 January 2023，
  
  Revised：03 June 2023，
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罗舒,宋怡,罗霞等.灵芝中10种灵芝酸类成分含量一测多评法的建立 ^Δ[J].中国药房,2023,34(14):1703-1706.

LUO Shu,SONG Yi,LUO Xia,et al.Establishment of quantitative analysis of multi-components by single marker for content determination of 10 ganoderic acids in Ganoderma lucidum[J].ZHONGGUO YAOFANG,2023,34(14):1703-1706.
罗舒,宋怡,罗霞等.灵芝中10种灵芝酸类成分含量一测多评法的建立 ^Δ[J].中国药房,2023,34(14):1703-1706. DOI： 10.6039/j.issn.1001-0408.2023.14.08.

LUO Shu,SONG Yi,LUO Xia,et al.Establishment of quantitative analysis of multi-components by single marker for content determination of 10 ganoderic acids in Ganoderma lucidum[J].ZHONGGUO YAOFANG,2023,34(14):1703-1706. DOI： 10.6039/j.issn.1001-0408.2023.14.08.

摘要

目的

建立同时测定灵芝中10种灵芝酸类成分的一测多评法。

方法

采用高效液相色谱（HPLC）法，以灵芝酸A为内参物，分别计算灵芝酸B、灵芝酸C

、灵芝酸D、灵芝酸F、灵芝酸H、灵芝烯酸A、灵芝烯酸B、灵芝烯酸C、灵芝烯酸D 9种成分的相对校正因子，用相对校正因子计算上述灵芝酸类成分的含量，并与外标法测定结果进行比较。

结果

灵芝酸A、灵芝酸B、灵芝酸C

、灵芝酸D、灵芝酸F、灵芝酸H、灵芝烯酸A、灵芝烯酸B、灵芝烯酸C、灵芝烯酸D的线性范围分别为0.032～3.996、0.040～4.971、0.037～4.568、0.028～3.558、0.033～4.177、0.044～5.440、0.032～3.944、0.040～4.994、0.045～5.593、0.035～4.342 mg/mL（

均不小于0.999 2）；精密度、稳定性（24 h）、重复性试验的RSD均小于2%；平均加样回收率分别为99.43%、100.25%、98.50%、99.88%、100.59%、99.64%、98.50%、99.40%、99.64%、99.76%（RSD＜2%，

＝6）。灵芝酸B、灵芝酸C

、灵芝酸D、灵芝酸F、灵芝酸H、灵芝烯酸A、灵芝烯酸B、灵芝烯酸C、灵芝烯酸D的相对校正因子平均值分别是1.788 5、1.288 2、1.126 4、1.698 5、0.885 4、5.468 1、4.210 9、5.780 8、4.290 3；不同产地灵芝样品一测多评法所得含量与外标法的相对误差均在±12%之内。

结论

以灵芝酸A为内参物同时测定灵芝中10种灵芝酸类成分含量的一测多评法是可行的，该方法准确性和重复性良好，可用于灵芝的质量控制。

Abstract

OBJECTIVE

To establish a quantitative analysis of multi-components by single marker （QAMS） method for simultaneous determination of 10 ganoderic acids in

Ganoderma lucidum

METHODS

Using ganoderic acid A as internal reference， high-performance liquid chromatography （HPLC） method was adopted to calculate relative correction factors of the other 9 components， such as ganoderic acid B， ganoderic acid C

， ganoderic acid D， ganoderic acid F， ganoderic acid H， ganoderenic acid A， ganoderenic acid B， ganoderenic acid C， ganoderenic acid D； the contents of above ganoderic acids were calculated with relative correction factors， and compared with the results of external standard method.

RESULTS

The linear relationship of ganoderic acid A， ganoderic acid B， ganoderic acid C

， ganoderic acid D， ganoderic acid F， ganoderic acid H， ganoderenic acid A， ganoderenic acid B， ganoderenic acid C and ganoderenic acid D were 0.032-3.996， 0.040-4.971， 0.037-4.568， 0.028-3.558， 0.033-4.177， 0.044-5.440， 0.032-3.944， 0.040-4.994， 0.045-5.593 and 0.035-4.342 mg/mL （all

≥0.999 2）， respectively. RSDs of precision， stability （24 h） and reproducibility tests were all lower than 2%. Their average recovery rates were 99.43%， 100.25%， 98.50%， 99.88%， 100.59%， 99.64%， 98.50%， 99.40%， 99.64% and 99.76%， respectively （RSD＜2%，

＝6）. Relative correction factors of ganoderic acid B， ganoderic acid C

， ganoderic acid D， ganoderic acid F， ganoderic acid H， ganoderenic acid A， ganoderenic acid B， ganoderenic acid C and ganoderenic acid D were 1.788 5， 1.288 2， 1.126 4， 1.698 5， 0.885 4， 5.468 1， 4.210 9， 5.780 8， 4.290 3， respectively. Relative errors between the content obtained by QAMS method and external standard method for

G. lucidum

from different origins were within ±12%.

CONCLUSIONS

It is feasible that the contents of 10 ganoderic acids are determined simultaneously by QAMS method， using ganoderic acid A as internal reference. This method shows good precision and reproducibility and can be used for the quality control of

G. lucidum

关键词

灵芝酸灵芝一测多评法相对校正因子

Keywords

Ganoderma lucidumquantitative analysis of multi-components by single markerrelative correction factor

references

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Establishment of quantitative analysis of multi-components by single marker for content determination of 10 ganoderic acids in Ganoderma lucidum

DOI：10.6039/j.issn.1001-0408.2023.14.08

摘要

Abstract

关键词

Keywords

references