Establishment of HPLC fingerprints for <italic style="font-style: italic">Ardisia crenata</italic>， <italic style="font-style: italic">Sophora tonkinensis</italic> and their couplet medicines and content determination of 5 chemical components

CHEN Yun; SHI Hui; FENG Tingting; ZHANG Liyan; DONG Xiu; ZHANG Jinhe; HUANG Bei; ZHOU Ying

doi:10.6039/j.issn.1001-0408.2023.16.06

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Establishment of HPLC fingerprints for Ardisia crenata， Sophora tonkinensis and their couplet medicines and content determination of 5 chemical components

更新时间：2023-08-24

- Establishment of HPLC fingerprints for Ardisia crenata， Sophora tonkinensis and their couplet medicines and content determination of 5 chemical components
- ZHONGGUO YAOFANG Vol. 34, Issue 16, Pages: 1949-1954(2023)
- 作者机构：
  
  1.贵州中医药大学药学院，贵阳　550025
  2.贵州中医药大学药食两用资源应用与开发研究中心，贵阳　550025
  3.贵州三力制药股份有限公司，贵州安顺　561100
  4.贵州省药品监督管理局检查中心，贵阳　550025
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.16.06
  CLC： R917;R284.1
- Published：30 August 2023，
  
  Received：15 March 2023，
  
  Revised：12 July 2023，
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陈贇,石慧,俸婷婷等.朱砂根、山豆根及其药对的HPLC指纹图谱建立及5种化学成分的含量测定 ^Δ[J].中国药房,2023,34(16):1949-1954.

CHEN Yun,SHI Hui,FENG Tingting,et al.Establishment of HPLC fingerprints for Ardisia crenata， Sophora tonkinensis and their couplet medicines and content determination of 5 chemical components[J].ZHONGGUO YAOFANG,2023,34(16):1949-1954.
陈贇,石慧,俸婷婷等.朱砂根、山豆根及其药对的HPLC指纹图谱建立及5种化学成分的含量测定 ^Δ[J].中国药房,2023,34(16):1949-1954. DOI： 10.6039/j.issn.1001-0408.2023.16.06.

CHEN Yun,SHI Hui,FENG Tingting,et al.Establishment of HPLC fingerprints for Ardisia crenata， Sophora tonkinensis and their couplet medicines and content determination of 5 chemical components[J].ZHONGGUO YAOFANG,2023,34(16):1949-1954. DOI： 10.6039/j.issn.1001-0408.2023.16.06.

摘要

目的

建立朱砂根、山豆根及其药对的指纹图谱，同时测定其中5种成分的含量。

方法

以水为溶剂，结合冷冻干燥技术制备朱砂根、山豆根单煎冻干粉和朱砂根-山豆根药对合煎冻干粉样品；采用高效液相色谱（HPLC）法建立3种冻干粉样品的指纹图谱，并同法测定没食子酸等5种成分的含量。

结果

朱砂根、山豆根单煎冻干粉样品和朱砂根-山豆根药对合煎冻干粉样品分别有5、10、14个共有峰；与对照图谱的相似度均大于0.90；朱砂根-山豆根药对合煎冻干粉样品中，指认3号峰为没食子酸、4号峰为苦参碱、6号峰为氧化苦参碱、8号峰为岩白菜素、14号峰为三叶豆紫檀苷。朱砂根单煎冻干粉样品中，没食子酸、岩白菜素的平均含量分别为0.499 3、4.962 6 mg/g；山豆根单煎冻干粉样品中，苦参碱、氧化苦参碱、三叶豆紫檀苷的平均含量分别为3.046 0、2.336 6、0.278 6 mg/g；朱砂根-山豆根药对合煎冻干粉样品中，没食子酸、苦参碱、氧化苦参碱、岩白菜素、三叶豆紫檀苷的平均含量分别为0.560 6、2.548 7、1.382 2、5.960 7、0.279 1 mg/g，转移率为8.87%～513.19%。

结论

所建立指纹图谱及含量测定方法稳定、可行，可用于朱砂根、山豆根及朱砂根-山豆根药对的质量控制；朱砂根-山豆根药对合煎冻干粉样品中苦参碱、氧化苦参碱的平均含量降低。

Abstract

OBJECTIVE

To establish the fingerprints of

Ardisia crenata

，

Sophora tonkinensis

and their couplet medicines， and to determine the contents of five components in them.

METHODS

Using water as solvent， single lyophilized powder of

A. crenata

and

S. tonkinensis

and combined lyophilized powder of their couplet medicines were prepared by combining lyophilization technology. The fingerprints of three lyophilized powder samples were established by using high-performance liquid chromatography （HPLC）， and the contents of 5 kinds of components such as gallic acid were determined simultaneously.

RESULTS

There were 5， 10 and 14 common peaks in the fingerprints for single lyophilized powder of

A. crenata

and

S. tonkinensis

and combined lyophilized powder of their couplet medicines； the similarities of them with the control fingerprints were all greater than 0.90. For combined lyophilized powder of couplet medicines， peak 3 was identified as gallic acid， peak 4 as matrine， peak 6 as oxymatrine， peak 8 as bergenin， and peak 14 as trifolirhizin. In single lyophilized powder of

A. crenata

， the average contents of gallic acid and bergenin were 0.499 3 and 4.962 6 mg/g， respectively. In single lyophilized powder of

S. tonkinensis

， the average contents of matrine， oxymatrine and trifolirhizin were 3.046 0， 2.336 6 and 0.278 6 mg/g， respectively. In combined lyophilized powder of couplet medicines， the average contents of gallic acid， matrine， oxymatrine， bergenin and trifolirhizin were 0.560 6， 2.548 7， 1.382 2， 5.960 7 and 0.279 1 mg/g， respectively. The transfer rates were 8.87%-513.19%.

CONCLUSIONS

The established fingerprint and content determination methods are stable and feasible， and can be used for the quality control of

A. crenata

and

S. tonkinensis

and their couplet medicines. The average contents of matrine and oxymatrine in combined lyophilized powder of

A. crenata

S. tonkinensis

couplet medicines are decreased.

关键词

朱砂根山豆根药对指纹图谱含量测定高效液相色谱法

Keywords

Sophora tonkinensiscouplet medicinesfingerprintcontent determinationhigh-performance liquid chromatography

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Establishment of HPLC fingerprints for Ardisia crenata， Sophora tonkinensis and their couplet medicines and content determination of 5 chemical components

DOI：10.6039/j.issn.1001-0408.2023.16.06

摘要

Abstract

关键词

Keywords

references