- Journal Information
- Archive
- Submission
- For Reviewers
- Statement and Policies
- 个人中心
- 退出登录
浏览全部资源
扫码关注微信
- Journal Information
- Journal Introduction
- Editorial Board
- Youth Editorial Board
- Editorial Board Charter
- Subscriptions
- Archive
- Current Issue
- Past Issues
- Priority Publication
- Virtual Collection
- Recommended Articles
- Submission
- Submission Process
- Submission Guidelines
- Download
- Contact us
- For Reviewers
- Guidelines for Reviewers
- Peer Reviewed Policies
- Statement and Policies
- Ethics and Malpractice
- Research Ethics Policies
- Repetition Detection
- Corrections and Retractions
- Editorial Policies
- Open Access Statement
- Website Responsibility
Effects of Cistanches Herba polysaccharides on rats with constipation-predominant irritable bowel syndrome
- ZHONGGUO YAOFANG Vol. 34, Issue 18, Pages: 2208-2212(2023)
- 作者机构：
1.甘肃省药品监督管理局信息中心统计咨询科，兰州 730070
2.武威市中医医院药剂科，甘肃 武威 733000
3.甘肃省中医院消化内科，兰州 730050
4.甘肃省药品监督管理局审核查验中心药品检查科，兰州 730070
5.甘肃省药品检验研究院中成药检验研究室，兰州 730070
- 作者简介：
- 基金信息：
DOI：10.6039/j.issn.1001-0408.2023.18.07
CLC： R965;R574Received：18 February 2023，
Revised：2023-07-10，
Accepted：27 July 2023，
Published：30 September 2023
- 稿件说明：
移动端阅览
刘延忠,贾新梅,郭洪章,等.肉苁蓉多糖对便秘型肠易激综合征大鼠的影响[J].中国药房,2023,34(18):2208-2212.
LIU Yanzhong,JIA Xinmei,GUO Hongzhang,et al.Effects of Cistanches Herba polysaccharides on rats with constipation-predominant irritable bowel syndrome[J].ZHONGGUO YAOFANG,2023,34(18):2208-2212.
刘延忠,贾新梅,郭洪章,等.肉苁蓉多糖对便秘型肠易激综合征大鼠的影响[J].中国药房,2023,34(18):2208-2212. DOI： 10.6039/j.issn.1001-0408.2023.18.07.
LIU Yanzhong,JIA Xinmei,GUO Hongzhang,et al.Effects of Cistanches Herba polysaccharides on rats with constipation-predominant irritable bowel syndrome[J].ZHONGGUO YAOFANG,2023,34(18):2208-2212. DOI： 10.6039/j.issn.1001-0408.2023.18.07.
摘要
目的
2
探讨肉苁蓉多糖（简称CDPS）对便秘型肠易激综合征（IBS-C）大鼠的影响。
方法
2
将SD大鼠分为对照组（20只）和造模组。造模组大鼠灌胃0～4 ℃生理盐水2 mL（每天1次，连续14 d）诱导IBS-C模型。将造模成功的大鼠分为模型组、阳性对照组（枸橼酸莫沙必利，1.35 mg/kg）和CDPS低、高剂量组（50、100 mg/kg），每组20只。各药物组大鼠灌胃相应药液，对照组和模型组大鼠灌胃等体积水，每次1次，持续28 d。检测各组大鼠的粪便含水量、血清5-羟色胺（5-HT）含量、炭末推进率，观察其结肠组织病理形态并检测结肠组织中神经生长因子（NGF）、原肌球蛋白受体激酶A（TrkA）mRNA及蛋白的表达。
结果
2
与对照组比较，模型组大鼠的粪便含水量和炭末推进率均显著降低（
P
＜0.05），结肠组织黏膜肌层断裂，炎症细胞浸润和细胞水肿明显，血清5-HT含量和结肠组织中NGF、TrkA mRNA及蛋白的相对表达量均显著升高（
P
＜0.05）。与模型组比较，各药物组大鼠的粪便含水量和炭末推进率均显著升高（
P
＜0.05），结肠组织病理改变明显减轻，血清5-HT含量和结肠组织中NGF、TrkA mRNA及蛋白的相对表达量均显著降低（
P
＜0.05），其中，阳性对照组和CDPS高剂量组的上述指标普遍接近于对照组（
P
＞0.05）。
结论
2
CDPS可缓解IBS-C大鼠的症状，该作用可能与NGF/TrkA信号通路被抑制有关。
Abstract
OBJECTIVE
2
To investigate the effects of Cistanches Herba polysaccharides （abbreviated as CDPS） on rats with constipation-predominant irritable bowel syndrome （IBS-C）.
METHODS
2
SD rats were divided into control group （20 rats） and modeling group. The modeling group was given 2 mL of normal saline at 0-4 ℃ intragastrically （once a day， for 14 consecutive days） to induce IBS-C model. After modeling， model rats were grouped into model group， positive control group （mosapride citrate， 1.35 mg/kg）， CDPS low-dose group （50 mg/kg） and CDPS high-dose group （100 mg/kg）， with 20 rats in each group. Administration groups were given corresponding drug solution intragastrically， and control group and model group were given a constant volume of water intragastrically， once a day， for 28 consecutive days. The fecal water content， serum content of 5-hydroxytryptamine （5-HT）， and charcoal powder propulsion rate of rats were determined in each group；the pathological morphology of colon tissue was observed， and mRNA and protein expressions of nerve growth factor （NGF） and tropomyosin receptor kinase A （TrkA） in colon tissue were detected.
RESULTS
2
Compared with control group， the fecal water content and carbon powder propulsion rate in the model group were decreased significantly （
P
＜0.05）； the rupture of mucosal muscle layer in colon tissue， significant infiltration of inflammatory cells and cellular edema were observed； the content of 5-HT in serum， and relative mRNA and protein expressions of NGF and TrkA were increased significantly （
P
＜0.05）. Compared with model group， the fecal water content and carbon powder propulsion rate of rats were increased significantly in administration groups （
P
＜0.05）， and pathological changes of colon tissue were relieved significantly， while the content of 5-HT in serum， the mRNA and protein expressions of NGF and TrkA in colon tissue were decreased significantly （
P
＜0.05）； among them， the above indicators in the positive control group and CDPS high-dose group were generally close to those in the control group （
P
＞0.05）.
CONCLUSIONS
2
CDPS can alleviate the symptoms of IBS-C rats， which may be related to the inhibition of NGF/TrkA signaling pathway.
关键词
Keywords
references
MOUREY F ， DECHERF A ， JEANNE J F ， et al . Saccharomyces cerevisiae I-3856 in irritable bowel syndrome with predominant constipation [J ] . World J Gastroenterol ， 2022 ， 28 （ 22 ）： 2509 - 2522 .
JAFARI S ， SAJEDI B ， JAMESHORANI M ， et al . Comparison of fluoxetine and duloxetine hydrochloride therapeutic effects on patients with constipation-predominant irritable bowel syndrome [J ] . Gastroenterol Hepatol Bed Bench ， 2022 ， 15 （ 1 ）： 45 - 52 .
YAO C J ， LI Y L ， PU M J ， et al . Traditional Chinese medicine for irritable bowel syndrome：a protocol for meta-analysis [J ] . Medicine（Baltimore） ， 2020 ， 99 （ 48 ）： e23394 .
LI Y L ， ZHANG Y L ， SU X M ， et al . Effects of two kinds of extracts of Cistanche deserticola on intestinal microbiota and its metabolism [J ] . Foods ， 2022 ， 11 （ 18 ）： 2897 .
GAO Y ， LI B ， LIU H ， et al . Cistanche deserticola polysaccharides alleviate cognitive decline in aging model mice by restoring the gut microbiota-brain axis [J ] . Aging （Albany NY） ， 2021 ， 13 （ 11 ）： 15320 - 15335 .
TRIACA V ， FICO E ， SPOSATO V ， et al . hNGF peptides elicit the NGF-TrkA signalling pathway in cholinergic neurons and retain full neurotrophic activity in the DRG assay [J ] . Biomolecules ， 2020 ， 10 （ 2 ）： 216 .
WONG H L X ， QIN H Y ， TSANG S W ， et al . Early life stress disrupts intestinal homeostasis via NGF-TrkA signaling [J ] . Nat Commun ， 2019 ， 10 （ 1 ）： 1745 .
ZHANG H ， XIANG Z ， DUAN X ， et al . Antitumor and anti-inflammatory effects of oligosaccharides from Cistanche deserticola extract on spinal cord injury [J ] . Int J Biol Macromol ， 2019 ， 124 ： 360 - 367 .
刘媛 ， 唐洪梅 ， 钟如帆 ， 等 . 健脾通腑颗粒对肝郁脾虚型便秘型肠易激综合征大鼠模型的作用及机制研究 [J ] . 中华中医药学刊 ， 2018 ， 36 （ 12 ）： 2870 - 2874 .
吴艳英 ， 任晓暄 ， 郭孟玮 ， 等 . 电针足三里、合谷穴对IBS模型大鼠肠道痛敏及动力异常治疗效应的比较研究 [J ] . 北京中医药大学学报 ， 2017 ， 40 （ 6 ）： 514 - 521 .
刘梦茹 ， 邱仁静 ， 李慧 ， 等 . 运脾柔肝方对便秘型肠易激综合征大鼠SCF/c-kit信号通路的影响 [J ] . 时珍国医国药 ， 2020 ， 31 （ 11 ）： 2593 - 2596 .
XIAO L ， LIU Q ， LUO M ， et al . Gut microbiota-derived metabolites in irritable bowel syndrome [J ] . Front Cell Infect Microbiol ， 2021 ， 11 ： 729346 .
LIU J ， LV C L ， WANG W ， et al . Slow，deep breathing intervention improved symptoms and altered rectal sensiti-vity in patients with constipation-predominant irritable bowel syndrome [J ] . Front Neurosci ， 2022 ， 16 ： 1034547 .
王金鑫 ， 谢胜 ， 黄茂光 ， 等 . 肠紧密连接在腹泻型肠易激综合征中的作用及中医药干预的研究进展 [J ] . 中国实验方剂学杂志 ， 2023 ， 29 （ 2 ）： 253 - 259 .
LIU X J ， JIAN C ， LI M Y ， et al . Microbiome-metabolomics deciphers the effects of Cistanche deserticola polysaccharides on aged constipated rats [J ] . Food Funct ， 2022 ， 13 （ 7 ）： 3993 - 4008 .
LI W T ， LUO Q Q ， WANG B ， et al . Bile acids induce visceral hypersensitivity via mucosal mast cell-to-nociceptor signaling that involves the farnesoid X receptor/nerve growth factor/transient receptor potential vanilloid 1 axis [J ] . FASEB J ， 2019 ， 33 （ 2 ）： 2435 - 2450 .
TAN X ， PEI W J ， XIE C E ， et al . Tongxie anchang decoction relieves visceral hypersensitivity in diarrhea-pre-dominant irritable bowel syndrome rats by regulating the NGF/TrkA signaling pathway [J ] . Evid Based Complement Alternat Med ， 2021 ， 2021 ： 6679348 .
CHOW C F W ， CHE S J ， QIN H Y ， et al . From psycho-logy to physicality：how nerve growth factor transduces early life stress into gastrointestinal motility disorders later in life [J ] . Cell Cycle ， 2019 ， 18 （ 16 ）： 1824 - 1829 .
JIANG L Q ， YE B X ， WANG Y ， et al . Effect and mechanisms of sacral nerve stimulation on visceral hypersensitivity mediated by nerve growth factor [J ] . J Cell Mol Med ， 2019 ， 23 （ 12 ）： 8019 - 8024 .
WANG F J ， TU P F ， ZENG K W ， et al . Total glycosides and polysaccharides of Cistanche deserticola prevent osteoporosis by activating Wnt/β-catenin signaling pathway in SAMP6 mice [J ] . J Ethnopharmacol ， 2021 ， 271 ： 113899 .
王景博 ， 韦春珠 ， 潘茂华 ， 等 . 活血化瘀方剂对重型颅脑损伤大鼠Wnt/β-连环蛋白信号通路表达的影响 [J ] . 中华危重病急救医学 ， 2020 ， 32 （ 9 ）： 1101 - 1106 .
0
Views
227
下载量
0
CSCD
- L-PDFDownload
- WORDDownload
- Meta-XMLDownload
- BibTeXDownload
- EndnoteDownload
- RefMan Download
- NoteExpressDownload
- NoteFirstDownload
Publicity Resources
Related Articles
Related Author
Related Institution
- Address：8th Floor, Sihuan Building, No. 129 Daping Main Street, Yuzhong District, Chongqing, China Postal code：400042
- Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号
公网安备50010302001817 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.