Protective effects of mangiferin against myocardial cell damage and its effects on the expression of NFATc4

WEI Qiqiu; GAO Si; FENG Yiping; PEI Shicheng; LIU Xueping

doi:10.6039/j.issn.1001-0408.2023.20.02

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Protective effects of mangiferin against myocardial cell damage and its effects on the expression of NFATc4

更新时间：2023-10-25

- Protective effects of mangiferin against myocardial cell damage and its effects on the expression of NFATc4
- ZHONGGUO YAOFANG Vol. 34, Issue 20, Pages: 2454-2458(2023)
- 作者机构：
  
  广西科技大学医学部药学系，广西柳州　545005
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.20.02
  CLC： R965
- Published：30 October 2023，
  
  Received：16 May 2023，
  
  Revised：27 August 2023，
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韦啟球,高思,冯艺萍等.芒果苷对心肌细胞损伤的保护作用及对NFATc4表达的影响 ^Δ[J].中国药房,2023,34(20):2454-2458.

WEI Qiqiu,GAO Si,FENG Yiping,et al.Protective effects of mangiferin against myocardial cell damage and its effects on the expression of NFATc4[J].ZHONGGUO YAOFANG,2023,34(20):2454-2458.
韦啟球,高思,冯艺萍等.芒果苷对心肌细胞损伤的保护作用及对NFATc4表达的影响 ^Δ[J].中国药房,2023,34(20):2454-2458. DOI： 10.6039/j.issn.1001-0408.2023.20.02.

WEI Qiqiu,GAO Si,FENG Yiping,et al.Protective effects of mangiferin against myocardial cell damage and its effects on the expression of NFATc4[J].ZHONGGUO YAOFANG,2023,34(20):2454-2458. DOI： 10.6039/j.issn.1001-0408.2023.20.02.

摘要

目的

研究芒果苷对过氧化氢（H

）诱导的心肌细胞氧化应激损伤的保护作用，以及对活化T细胞核因子c4（NFATc4）表达的影响。

方法

体外培养心肌H9c2细胞，分为空白组、H

组和芒果苷50、100、150 μmol/L组，芒果苷组细胞在经不同浓度芒果苷作用12 h后，再与H

组一同经H

（200 μmol/L）刺激12 h，检测各组细胞的相对存活率，细胞上清液中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）水平，细胞中活性氧（ROS）水平，以及细胞中凋亡相关蛋白[B细胞淋巴瘤2（Bcl-2）、Bcl-2相关X蛋白（Bax）、剪切的胱天蛋白酶3（cleaved caspase-3）]、核蛋白NFATc4的表达情况。转染NFATc4干扰序列，考察NFATc4对H

诱导的心肌细胞中氧化应激指标和凋亡相关蛋白的影响。

结果

与空白组比较，H

组细胞的相对存活率，SOD、CAT水平，Bcl-2的相对表达量均显著降低；MDA、ROS水平，Bax、cleaved caspase-3和核蛋白NFATc4的相对表达量均显著升高（

＜0.05或

＜0.01）。与H

组比较，芒果苷100、150 μmol/L组细胞的上述指标均得以显著逆转（

＜0.05）。转染NFATc4干扰序列后，核蛋白NFATc4的表达显著下调，MDA、ROS水平和Bax、cleaved caspase-3蛋白的表达均较H

组显著降低/下调，SOD、CAT水平和Bcl-2蛋白的表达均较H

组显著升高/上调（

＜0.05）。

结论

芒果苷能够减轻H

诱导的H9c2细胞氧化应激，减少细胞凋亡，抑制NFATc4蛋白入核，进而缓解心肌细胞损伤；降低NFATc4蛋白的核内水平与减轻H

诱导的氧化应激、细胞凋亡有关。

Abstract

OBJECTIVE

To study the protective effects of mangiferin against oxidative stress injury of myocardial cells induced by hydrogen peroxide （H

）， and its effects on the expression of nuclear factor of activated T cell cytoplasmic 4（NFATc4）.

METHODS

H9c2 myocardial cells were cultured

in vitro

and divided into blank group， H

group， and 50， 100， 150 μmol/L mangiferin groups. Mangiferin groups were treated with different concentrations of mangiferin for 12 h， and then were subjected to H

（200 μmol/L） stimulation for 12 hours together with the H

group； relative survival rate was detected in each group， and the levels of superoxide dismutase （SOD）， catalase （CAT） and malondialdehyde （MDA） in cell supernatant and reactive oxygen species （ROS） in H9c2 cells were measured. Meanwhile， the expressions of apoptosis-related proteins [B cell lymphoma 2 （Bcl-2）， Bcl-2 associated X protein （Bax）， cleaved caspase-3] and nuclear protein NFATc4 were determined. Furthermore， the NFATc4 interference sequence was transfected， and the effects of NFATc4 on oxidant stress indexes and apoptosis-related proteins in H

-induced myocardial cells were investigated.

RESULTS

Compared with blank group， relative cell viability， the levels of SOD and CAT， relative expression of Bcl-2 were decreased significantly， while the levels of MDA and ROS， relative expressions of Bax， cleaved caspase-3 and nuclear protein NFATc4 were increased significantly （

＜0.05 or

＜0.01）. Compared with the H

group， the above indexes of 100 and 150 μmol/L mangiferin groups were reversed significantly （

＜0.05）. After the transfection of the NFATc4 interference sequence， the expression of nuclear protein NFATc4 was down-regulated significantly； the levels of MDA and SOD， the protein expressions of Bax and cleaved caspase-3 were all decreased/down-regulated significantly， while the levels of SOD and CAT， and the protein expression of Bcl-2 were all increased/up-regulated significantly， compared with H

group （

＜0.05）.

CONCLUSIONS

Mangiferin can relieve H

-induced oxidative stress of H9c2 cells， reduce the apoptosis and inhibit the nuclear translocation of NFATc4， thereby alleviating myocardial cell damage； reducing the nuclear level of NFATc4 protein is related to reducing H

-induced oxidative stress and cell apoptosis.

关键词

芒果苷H9c2细胞氧化应激凋亡活化T细胞核因子c4

Keywords

H9c2 cellsoxidative stressapoptosisnuclear factor of activated T cells cytoplasmic 4

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