Establishment of UPLC fingerprint and study on anti-respiratory syncytial virus spectrum-effective relationship of ethanol extract from<italic style="font-style: italic"> Callicarpa nudiflora</italic>

SHAO Bingmei; LI Xiaofeng; YAN Beibei; LIU Qifeng; QIN Huanyun; ZHAO Xiangjun; HOU Lin

doi:10.6039/j.issn.1001-0408.2023.20.04

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Establishment of UPLC fingerprint and study on anti-respiratory syncytial virus spectrum-effective relationship of ethanol extract from Callicarpa nudiflora

更新时间：2023-10-25

- Establishment of UPLC fingerprint and study on anti-respiratory syncytial virus spectrum-effective relationship of ethanol extract from Callicarpa nudiflora
- ZHONGGUO YAOFANG Vol. 34, Issue 20, Pages: 2465-2470(2023)
- 作者机构：
  
  1.山东中医药大学青岛中医药科学院，山东青岛　266000
  2.江西普正制药股份有限公司国家企业技术中心，江西吉安　343100
  3.山东中医药大学药学院，济南　250300
  4.泰安市立医院神经外科，山东泰安　271000
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.20.04
  CLC： R917;R978.7
- Published：30 October 2023，
  
  Received：03 April 2023，
  
  Revised：04 September 2023，
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邵冰梅,李小锋,闫蓓蓓等.裸花紫珠醇提物UPLC指纹图谱建立及抗呼吸道合胞病毒谱效关系研究 ^Δ[J].中国药房,2023,34(20):2465-2470.

SHAO Bingmei,LI Xiaofeng,YAN Beibei,et al.Establishment of UPLC fingerprint and study on anti-respiratory syncytial virus spectrum-effective relationship of ethanol extract from Callicarpa nudiflora[J].ZHONGGUO YAOFANG,2023,34(20):2465-2470.
邵冰梅,李小锋,闫蓓蓓等.裸花紫珠醇提物UPLC指纹图谱建立及抗呼吸道合胞病毒谱效关系研究 ^Δ[J].中国药房,2023,34(20):2465-2470. DOI： 10.6039/j.issn.1001-0408.2023.20.04.

SHAO Bingmei,LI Xiaofeng,YAN Beibei,et al.Establishment of UPLC fingerprint and study on anti-respiratory syncytial virus spectrum-effective relationship of ethanol extract from Callicarpa nudiflora[J].ZHONGGUO YAOFANG,2023,34(20):2465-2470. DOI： 10.6039/j.issn.1001-0408.2023.20.04.

摘要

目的

建立裸花紫珠醇提物的指纹图谱，分析其体外抗呼吸道合胞病毒（RSV）活性并进行谱效关系研究。

方法

以10%、30%、50%、70%、90%乙醇为溶剂，制备4批裸花紫珠药材的20批醇提物。采用超高效液相色谱法，利用《中药色谱指纹图谱相似度评价系统（2012版）》绘制20批裸花紫珠醇提物的指纹图谱并进行相似度评价；采用致细胞病变效应法和MTT法考察裸花紫珠醇提物对RSV的体外抑制活性；运用Pearson相关性分析、灰色关联度分析、正交偏最小二乘（OPLS）分析进行谱效关系研究。

结果

20批裸花紫珠醇提物有25个共有峰，相似度为0.912～0.998，共有峰峰面积的RSD为33.54%～162.28%。20批裸花紫珠醇提物对RSV的平均半数抑制浓度为9.55～272.23 μg/mL；Pearson相关性分析、灰色关联度分析、OPLS分析结果表明，共有峰8、10、12、16、18～19、22～24与药效指标的Pearson相关系数（

＜0.05）和回归系数均为负值，关联度均大于0.6，变量重要性投影值均大于1。

结论

20批裸花紫珠醇提物成分相似但含量差异明显，且具有不同程度的体外抗RSV活性，其中共有峰8、10、12、16、18～19、22～24对应成分可能是裸花紫珠抗RSV的特征成分。

Abstract

OBJECTIVE

To establish the fingerprint of the ethanol extract from

Callicarpa nudiflora

， analyze its anti-respiratory syncytial virus （RSV） activity

in vitro

， and study the relationship between spectrum and effect.

METHODS

Using 10%， 30%， 50%， 70% and 90% ethanol as solvent， 20 batches of ethanol extracts from 4 batches of

C. nudiflora

were prepared. The fingerprints for 20 batches of ethanol extracts from

C. nudiflora

were mapped by ultra-high-performance liquid chromatography （UPLC）， and the similarity evaluation was conducted by using the

Similarity Evaluation System for Traditional Chinese Medicine Chromatographic Fingerprints

（2012 edition）. The cytopathic effect method and MTT method were used to investigate the

in vitro

inhibitory activity of the ethanol extracts from

C. nudiflora

on RSV. Pearson correlation analysis， grey correlation degree and orthogonal partial least squares （OPLS） analysis were used to study the spectrum-effect relationship.

RESULTS

There were 25 common peaks in 20 batches of ethanol extracts from

C. nudiflora

， and the similarities ranged from 0.912 to 0.998， and the RSDs of common peak areas were 33.54%-162.28%. The average values of IC

for RSV of 20 batches of ethanol extracts from

C. nudiflora

were 9.55-272.23 μg/mL. The results of Pearson correlation analysis， grey correlation analysis and OPLS analysis showed that the Pearson correlation coefficients （

＜0.05） of the common peaks 8， 10， 12， 16， 18-19， 22-24 with pharmacodynamic indicators and regression coefficients were all negative， the correlation coefficients were all greater than 0.6， and the values of variable importance in projection were all greater than 1.

CONCLUSIONS

Twenty batches of ethanol extracts from

C. nudiflora

have similar components but significant differences in content， and exhibit different degrees of anti-RSV activity

in vitro

. The corresponding components of common peaks 8， 10， 12， 16， 18-19， 22-24 may be the characteristic components of anti-RSV of

C. nudiflora

关键词

裸花紫珠醇提物呼吸道合胞病毒指纹图谱抗病毒谱效关系

Keywords

ethanol extractrespiratory syncytial virusfingerprintanti-virusspectrum-effect relationship

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Related Institution

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Establishment of UPLC fingerprint and study on anti-respiratory syncytial virus spectrum-effective relationship of ethanol extract from Callicarpa nudiflora

DOI：10.6039/j.issn.1001-0408.2023.20.04

摘要

Abstract

关键词

Keywords

references