Effects and mechanism of ginsenoside Rh<sub>2</sub> on the proliferation and apoptosis of human glioma cells

LIU Ying; TAN Hui; XIA Jing; XIONG Wei; DENG Xuesong

doi:10.6039/j.issn.1001-0408.2023.20.05

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Effects and mechanism of ginsenoside Rh₂ on the proliferation and apoptosis of human glioma cells

更新时间：2023-10-25

- Effects and mechanism of ginsenoside Rh₂ on the proliferation and apoptosis of human glioma cells
- ZHONGGUO YAOFANG Vol. 34, Issue 20, Pages: 2471-2475(2023)
- 作者机构：
  
  1.重庆三峡医药高等专科学校基础医学部，重庆　404120
  2.重庆市抗肿瘤天然药物工程研究中心，重庆　404120
  3.三峡库区道地药材开发利用重庆市重点实验室，重庆　404120
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.20.05
  CLC： R965;R739.41
- Published：30 October 2023，
  
  Received：05 April 2023，
  
  Revised：03 September 2023，
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刘滢,谭辉,夏菁等.人参皂苷Rh₂对胶质瘤细胞增殖、凋亡的影响及机制 ^Δ[J].中国药房,2023,34(20):2471-2475.

LIU Ying,TAN Hui,XIA Jing,et al.Effects and mechanism of ginsenoside Rh₂ on the proliferation and apoptosis of human glioma cells[J].ZHONGGUO YAOFANG,2023,34(20):2471-2475.
刘滢,谭辉,夏菁等.人参皂苷Rh₂对胶质瘤细胞增殖、凋亡的影响及机制 ^Δ[J].中国药房,2023,34(20):2471-2475. DOI： 10.6039/j.issn.1001-0408.2023.20.05.

LIU Ying,TAN Hui,XIA Jing,et al.Effects and mechanism of ginsenoside Rh₂ on the proliferation and apoptosis of human glioma cells[J].ZHONGGUO YAOFANG,2023,34(20):2471-2475. DOI： 10.6039/j.issn.1001-0408.2023.20.05.

摘要

目的

探讨人参皂苷Rh

对人胶质瘤U87、U251细胞增殖、凋亡的影响及可能机制。

方法

以人胶质瘤U87、U251细胞为对象，经不同浓度的人参皂苷Rh

作用后，检测细胞的增殖、凋亡情况以及细胞中组蛋白脱乙酰酶1（HDAC1）蛋白和凋亡相关蛋白[B细胞淋巴瘤2（Bcl-2）、Bcl-2相关X蛋白（Bax）、切割型胱天蛋白酶3（cleaved caspase-3）]的表达情况。

结果

10、20、30、40、50、60、70、80 μmol/L的人参皂苷Rh

均能显著升高U87、U251细胞的增殖抑制率（

＜0.05或

＜0.01），该成分对2种细胞作用48 h的半数抑制浓度分别为51.34、55.84 μmol/L；30、50 μmol/L的人参皂苷Rh

均能显著升高2种细胞的总凋亡率，能显著降低HDAC1、Bcl-2蛋白的表达水平，并显著升高Bax、cleaved caspase-3蛋白的表达水平（

＜0.05或

＜0.01）。

结论

人参皂苷Rh

可抑制2种人胶质瘤细胞增殖并促进其凋亡，其作用可能与下调HDAC1蛋白的表达和激活Bcl-2家族蛋白介导的凋亡途径有关。

Abstract

OBJECTIVE

To investigate the effects and mechanism of ginsenoside Rh

on the proliferation and apoptosis in human glioma U87 and U251 cells.

METHODS

Using human glioma U87 and U251 cells as subjects， the proliferation and apoptosis， as well as the expression of histone deacetylase 1 （HDAC1） protein and apoptosis-related proteins [B cell lymphoma-2 （Bcl-2）， Bcl-2-associated X protein （Bax） and cleaved caspase-3] were detected after being treated with different concentrations of ginsenoside Rh

RESULTS

The concentrations of 10， 20， 30， 40， 50， 60， 70， 80 μmol/L ginsenoside Rh

could generally significantly increase the proliferation inhibition rate of U87 and U251 cells （

＜0.05 or

＜0.01）， and the half inhibitory concentrations of this component after 48 hours of action were 51.34 and 55.84 μmol/L， respectively； 30， 50 μmol/L ginsenoside Rh

could increase the total apoptotic rate of both types of cells， reduced the protein expressions of HDAC1 and Bcl-2， and increased the protein expressions of Bax and cleaved caspase-3 significantly （

＜0.05 or

＜0.01）.

CONCLUSIONS

Ginsenoside Rh

has a significant inhibitory effect on the proliferation of glioma cells and promotes the apoptosis of cells， which may be through reducing the expression of HDAC1 protein and activating the Bcl-2 family protein-mediated apoptosis pathway.

关键词

人参皂苷Rh2胶质瘤组蛋白脱乙酰基酶1U87细胞U251细胞增殖凋亡

Keywords

ginsenoside Rh2gliomahistone deacetylase 1U87 cellsU251 cellsproliferationapoptosis

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Effects and mechanism of ginsenoside Rh2 on the proliferation and apoptosis of human glioma cells

DOI：10.6039/j.issn.1001-0408.2023.20.05

摘要

Abstract

关键词

Keywords

references

Effects and mechanism of ginsenoside Rh₂ on the proliferation and apoptosis of human glioma cells