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Improvement effect and mechanism of Baicao fuyanqing suppository on bacterial vaginitis in rats
- ZHONGGUO YAOFANG Vol. 34, Issue 20, Pages: 2476-2482(2023)
- 作者机构：
1.贵州医科大学药学院/药用植物功能与应用国家重点实验室，贵阳 550025
2.贵州医科大学微生物与生化制药工程中心，贵阳 550025
3.贵州医科大学实验动物中心，贵阳 550025
- 作者简介：
- 基金信息：
DOI：10.6039/j.issn.1001-0408.2023.20.06
CLC： R965Published：30 October 2023，
Received：06 April 2023，
Revised：31 August 2023，
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王棋,王鹏娇,孙小东等.百草妇炎清栓对大鼠细菌性阴道炎的改善作用及机制 Δ[J].中国药房,2023,34(20):2476-2482.
WANG Qi,WANG Pengjiao,SUN Xiaodong,et al.Improvement effect and mechanism of Baicao fuyanqing suppository on bacterial vaginitis in rats[J].ZHONGGUO YAOFANG,2023,34(20):2476-2482.
王棋,王鹏娇,孙小东等.百草妇炎清栓对大鼠细菌性阴道炎的改善作用及机制 Δ[J].中国药房,2023,34(20):2476-2482. DOI： 10.6039/j.issn.1001-0408.2023.20.06.
WANG Qi,WANG Pengjiao,SUN Xiaodong,et al.Improvement effect and mechanism of Baicao fuyanqing suppository on bacterial vaginitis in rats[J].ZHONGGUO YAOFANG,2023,34(20):2476-2482. DOI： 10.6039/j.issn.1001-0408.2023.20.06.
摘要
目的
2
探究百草妇炎清栓对大鼠细菌性阴道炎（BV）的改善作用及潜在机制。
方法
2
将雌性SD大鼠随机分为正常组，模型组，甲硝唑组（阳性对照，0.03 g/kg）和百草妇炎清栓低、中、高剂量组（0.18、0.36、0.72 g/kg），每组8只。除正常组外，其余各组大鼠均通过皮下注射苯甲酸雌二醇注射液0.2 g+经阴道口注入大肠埃希菌悬液（2×10
8
～3×10
8
cfu/mL）20 μL建立BV大鼠模型。各药物组大鼠经阴道给予相应药物，正常组和模型组大鼠给予生理盐水，每天1次，持续6 d。末次给药24 h后，对各组大鼠进行阴道外观评分并检测阴道pH，检测其阴道灌洗液中细胞因子[白细胞介素1β（IL-1β）、IL-2、IL-13、免疫球蛋白A（IgA）]水平，观察其子宫及附件形态和阴道组织病理改变，并检测其阴道组织中Toll样受体2（TLR2）、TLR4、核因子κB（NF-κB）蛋白的表达情况。
结果
2
与正常组比较，模型组大鼠子宫明显充血水肿，子宫及附件的形态不规则；阴道组织可见明显水肿增生、上皮细胞脱落，并伴有大量炎症细胞浸润；其阴道口红肿评分和分泌物评分，阴道pH，促炎因子IL-1β、IL-2水平和TLR2、TLR4、NF-κB蛋白的表达均显著升高/上调，IgA和抗炎因子IL-13水平显著降低（
P
＜0.05或
P
＜0.01）。与模型组比较，各药物组大鼠子宫及附件、阴道组织病变均有不同程度改善，上述大部分定量指标均显著好转（
P
＜0.05或
P
＜0.01）。
结论
2
百草妇炎清栓对BV大鼠炎症症状有一定的改善作用，其机制可能与抑制TLR/NF-κB信号通路有关。
Abstract
OBJECTIVE
2
To explore the improvement effect and potential mechanism of Baicao fuyanqing suppository on bacterial vaginitis （BV） in rats.
METHODS
2
The female SD rats were randomly divided into normal group， model group， metronidazole group （positive control， 0.03 g/kg）， Baicao fuyanqing suppository low-dose， medium-dose and high-dose groups （0.18， 0.36， 0.72 g/kg）， with 8 rats in each group. Except for the normal group， the rats in other groups were injected subcutaneously with 0.2 g of Estradiol benzoate injection+20 μL of
Escherichia coli
suspension （2×10
8
～3×10
8
cfu/mL） through the vaginal opening to establish the BV rat model. Administration groups were given relevant medicine vaginally， while the normal group and the model group were given normal saline， once a day， for 6 consecutive days. Twenty-four hours after the last administration， the vaginal appearance score and vaginal pH were measured for each group of rats. The levels of cytokines [interleukin-1β （IL-1β）， IL-2， IL-13， immunoglobulin A （IgA）] in vaginal lavage fluid were determined. The morphology of the uterus and accessories， and pathological changes in the vaginal tissue were observed. The expressions of Toll-like receptor 2 （TLR2）， TLR4 and nuclear factor-κB （NF-κB） in vaginal tissues were determined.
RESULTS
2
Compared with the normal group， the uterus edema， the irregular shape of uterus and accessories， the vaginal mucosa hyperplasia， and the massive desquamation of epithelial cells were observed in the model group， complicating with massive infiltration of inflammatory cells； vaginal opening redness and swelling score and secretion score， vaginal pH， the levels of proinflammatory cytokine IL-1β and IL-2， the protein expressions of TLR2， TLR4 and NF-κB were all increased or up-regulated， while the levels of IgA and anti-inflammatory cytokine IL-13 decreased significantly （
P
＜0.05 or
P
＜0.01）. Compared with the model group， varying degrees of improvement in uterine and accessories， and vaginal tissue lesions in rats were observed in administration groups， and most of the quantitative indicators mentioned above showed significant improvement （
P
＜0.05 or
P
＜0.01）.
CONCLUSIONS
2
Baicao fuyanqing suppository has a certain improvement effect on inflammatory symptoms in BV rats， and its mechanism may be related to the inhibition of the TLR/NF-κB signaling pathway.
关键词
百草妇炎清栓细菌性阴道炎炎症Toll样受体/核因子κB信号通路
Keywords
bacterial vaginitisinflammationToll-like receptor/nuclear factor-κB signaling pathway
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