To evaluate the efficacy of the functional dressing of
Polygonum capitatum
nanofibers (P-PVP-PCL).
METHODS
2
P-PVP-PCL were prepared by electrospinning technology, and the microstructure of P-PVP-PCL was observed. The antibacterial activity and antioxidant activity of P-PVP-PCL and its effects on the survival rate, adhesion and migration rate of mouse fibroblast L929 cells were investigated. The effects of medical gauze dressing, blank nanofiber dressing (PVP-PCL) and P-PVP-PCL on the healing rate of the wound were investigated by establishing the back skin wound model of rats. The pathological changes of the wound tissue and collagen fiber deposition were observed, as well as the number of platelet endothelial cell adhesion molecule-1 (CD31) positive blood vessels and the expression of transforming growth factor-β (TGF-β) protein in wound tissue.
RESULTS
2
P-PVP-PCL had a smooth surface and a double-layer structure at the cross-section. The inhibition rates of P-PVP-PCL against
Staphylococcus aureus
and
Escherichia coli
were (98.88±0.66)% and (94.75±1.41)%, respectively. The antioxidant activity of P-PVP-PCL was (83.69±1.56)%, and the cell activity of the P-PVP-PCL group was significantly higher than those of the control group and PVP-PCL group (
P
<0.05). Compared with medical gauze dressings, P-PVP-PCL was more conducive to L929 cell adhesion; at 48 hours, the cell scratches in this group had basically healed. Compared with the medical gauze dressing group, the wound healing rates of the PVP-PCL group and the P-PVP-PCL group were significantly increased (
P
<0.05). On the 14th day of intervention, the wounds in the P-PVP-PCL group had basically healed, there was no dermal necrosis in the wound tissue, and the collagen fibers were arranged relatively neatly and the density was relatively uniform. The number of CD31 positive blood vessels and the expression of TGF-β protein showed a downward trend compared with the 7th day of intervention, and the number of CD31 positive blood vessels was significantly lower than those of the medical gauze dressing group and PVP-PCL group (
P
<0.05), but the protein expression of TGF-β was significantly higher than those of the medical gauze dressing group and the PVP-PCL group (
P
<0.05).
CONCLUSIONS
2
P-PVP-PCL has good antibacterial and antioxidant activity
in vitro
, and can promote the proliferation, adhesion and migration of L929 cells. It can promote wound healing of rats
Guizhou Medical Products Administration. Quality standard of Chinese medicinal materials and national medicinal materials in Guizhou Province:2003 edition[S]. Guiyang:Guizhou Science and Technology Publishing House,2003:147.
TAO G,CAI R,WANG Y J,et al. Fabrication of antibacterial sericin based hydrogel as an injectable and mouldable wound dressing[J]. Mater Sci Eng C Mater Biol Appl,2021,119:111597.
ASIRI A,SAIDIN S,SANI M H,et al. Epidermal and fibroblast growth factors incorporated polyvinyl alcohol electrospun nanofibers as biological dressing scaffold[J]. Sci Rep,2021,11(1):5634.
LYU Y J,SUN C Y,LIU Z M,et al. Preparation and pro-perties of bilayer nanofibers functional dressing of Polygonum capitatum for wound nursing[J]. J Shenyang Pharm Univ,2023,40(6):705-714,724.
KAPAREKAR P S,PATHMANAPAN S,ANANDASADAGOPAN S K. Polymeric scaffold of gallic acid loaded chitosan nanoparticles infused with collagen-fibrin for wound dressing application[J]. Int J Biol Macromol,2020,165(Pt A):930-947.
AKBARI A,RABBANI S,IRANI S,et al. In vitro and in vivo study of carboxymethyl chitosan/polyvinyl alcohol for wound dressing application[J]. J Appl Polym Sci,2022,139(10):51764.
CHEN K,PAN H,JI D X,et al. Curcumin-loaded sandwich-like nanofibrous membrane prepared by electrospinning technology as wound dressing for accelerate wound healing[J]. Mater Sci Eng C Mater Biol Appl,2021,127:112245.
ZHANG J,ZHENG Y J,LEE J,et al. A pulsatile release platform based on photo-induced imine-crosslinking hydrogel promotes scarless wound healing[J]. Nat Commun,2021,12(1):1670.