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Mechanism study of chlorogenic acid alleviating macrophage activation
- ZHONGGUO YAOFANG Vol. 34, Issue 21, Pages: 2601-2607(2023)
- 作者机构：
1.中国人民解放军南部战区总医院麻醉科，广州 510501
2.中国人民解放军西部战区总医院骨科，成都 610083
- 作者简介：
- 基金信息：
DOI：10.6039/j.issn.1001-0408.2023.21.06
CLC： R965Published：15 November 2023，
Received：15 April 2023，
Revised：10 October 2023，
扫 描 看 全 文
郑薇,郎静,黄西凤等.绿原酸抑制巨噬细胞激活的机制研究 Δ[J].中国药房,2023,34(21):2601-2607.
ZHENG Wei,LANG Jing,HUANG Xifeng,et al.Mechanism study of chlorogenic acid alleviating macrophage activation[J].ZHONGGUO YAOFANG,2023,34(21):2601-2607.
郑薇,郎静,黄西凤等.绿原酸抑制巨噬细胞激活的机制研究 Δ[J].中国药房,2023,34(21):2601-2607. DOI： 10.6039/j.issn.1001-0408.2023.21.06.
ZHENG Wei,LANG Jing,HUANG Xifeng,et al.Mechanism study of chlorogenic acid alleviating macrophage activation[J].ZHONGGUO YAOFANG,2023,34(21):2601-2607. DOI： 10.6039/j.issn.1001-0408.2023.21.06.
摘要
目的
2
观察绿原酸对脂多糖（LPS）致巨噬细胞激活的影响，并探讨骨髓细胞2表达的触发受体（TREM2）蛋白在其中的作用。
方法
2
为筛选LPS造模浓度，分别以1、10、100 ng/mL的LPS培养细胞24 h，检测细胞培养上清液中白细胞介素6（IL-6）水平和细胞中诱导型一氧化氮合酶（iNOS）蛋白表达水平。为筛选绿原酸给药浓度，将细胞分为Control组、LPS处理组和3个不同浓度绿原酸（0.01、0.1、1 μmol/L）干预组，检测细胞培养上清液中肿瘤坏死因子α（TNF-α）、IL-1β水平和细胞中iNOS、TREM2蛋白表达水平以及细胞活力。为观察TREM2在绿原酸抑制巨噬细胞激活中的作用，采用TREM2小干扰RNA（TREM2-siRNA）干扰细胞TREM2蛋白表达，将细胞分为Control组、LPS处理组、绿原酸+LPS组、TREM2-siRNA+绿原酸+LPS组和乱序-siRNA（SC-siRNA）+绿原酸+LPS组，在含有上述药物的培养基/空白培养基中孵育24 h后，检测细胞培养上清液中TNF-α和IL-1β水平以及细胞中TREM2、iNOS和核因子κB p65（NF-κB p65）的蛋白表达水平。
结果
2
10 ng/mL LPS可显著促进细胞释放IL-6，并增加细胞中iNOS蛋白表达水平，故选择该质量浓度LPS进行后续实验。与LPS处理组相比，0.1 μmol/L绿原酸干预组细胞培养上清液中TNF-α、IL-1β水平和细胞中iNOS蛋白表达水平均显著降低（
P
＜0.05），细胞中TREM2蛋白表达水平显著升高（
P
＜0.05），细胞活力未受影响，故选择该浓度的绿原酸进行后续实验。在观察TREM2蛋白在绿原酸抑制巨噬细胞激活中的作用中发现，与Control组比较，LPS处理组细胞中iNOS和NF-κB p65蛋白表达水平均显著升高（
P
＜0.05）；与LPS处理组比较，绿原酸+LPS组细胞中iNOS和NF-κB p65蛋白表达水平均显著降低、TREM2蛋白表达水平显著升高（
P
＜0.05）；与绿原酸+LPS组比较，TREM2-siRNA+绿原酸+LPS组细胞中iNOS和NF-κB p65蛋白表达水平均显著升高、TREM2蛋白表达水平显著降低（
P
＜0.05），TREM2-siRNA明显逆转了绿原酸的上述作用；而SC-siRNA未对绿原酸产生的上述作用造成影响。
结论
2
绿原酸可显著抑制LPS对巨噬细胞的激活，其抗炎作用可能是通过TREM2蛋白介导的。
Abstract
OBJECTIVE
2
To observe the effects of chlorogenic acid on the activation of macrophage induced by lipopolysaccharide （LPS）， and to explore the role of triggering receptors expressed on myeloid cells-2 （TREM2） in the action.
METHODS
2
To find a suitable LPS concentration， the cells were cultured with 1， 10 and 100 ng/mL LPS for 24 h. The level of interleukin 6 （IL-6） in the cell culture supernatant and protein expression of inducible nitric oxide synthase （iNOS） in the cells were detected. To search for a suitable chlorogenic acid concentration， the cells were divided into control group， LPS group and three chlorogenic acid （0.01， 0.1 and 1 μmol/L）+LPS groups. The levels of tumor necrosis factor α （TNF-α） and IL-1β in the cell culture supernatant， the protein expressions of iNOS and TREM2 in the cells and cell viability were detected. To observe the effects of TREM2 in chlorogenic acid alleviating macrophage activation， TREM2-small interfering RNA （TREM2-siRNA） was taken to intervene in TREM2 protein expression. The cells were divided into control group， LPS group， chlorogenic acid+LPS group， TREM2-siRNA+chlorogenic acid+LPS group and SC-siRNA+chlorogenic acid+LPS group. After 24 h incubation， the levels of TNF-α and IL-1β in the cell culture supernatant and protein expressions of TREM2， iNOS and nuclear factor κB p65 （NF-κB p65） in the cells were detected.
RESULTS
2
10 ng/mL LPS promoted IL-6 release and increased iNOS protein expression， and 10 ng/mL LPS was taken in the next experiments. Compared with the LPS group， 0.1 μmol/L chlorogenic acid decreased TNF-α and IL-1β levels， and down-regulated iNOS expression， meanwhile increased TREM2 expression without effect on cell viability， and 0.1 μmol/L chlorogenic acid was taken in the next experiments. Compared with the control group， the protein expressions of iNOS and NF-κB p65 in the LPS group were significantly increased （
P
＜0.05）； compared with the LPS group， the protein expressions of iNOS and NF-κB p65 in the chlorogenic acid+LPS group were significantly decreased， the protein expressions of TREM2 was significantly increased （
P
＜0.05）； compared with the chlorogenic acid+LPS group， the protein expressions of iNOS and NF-κB p65 of TREM2-siRNA+chlorogenic acid+LPS group were significantly increased， the protein expressions of TREM2 was significantly decreased （
P
＜0.05）. TREM2-siRNA could significantly reverse the above effects of chlorogenic acid， while SC-siRNA did not significantly affect the above anti-inflammatory effects of chlorogenic acid.
CONCLUSIONS
2
Chlorogenic acid can inhibit the LPS-induced macrophage activation， and its anti-inflammatory may be mediated by TREM2 protein.
关键词
绿原酸巨噬细胞脂多糖炎症骨髓细胞2表达的触发受体
Keywords
macrophagelipopolysaccharideinflammationtriggering receptors expressed on myeloid cells-2
references
SCHERAGA R G，SOUTHERN B D，GROVE L M，et al. The role of TRPV4 in regulating innate immune cell function in lung inflammation[J]. Front Immunol，2020，11：1211.
LEE H W，FESSLER M B，QU P，et al. Macrophage polarization in innate immune responses contributing to pathogenesis of chronic kidney disease[J]. BMC Nephrol，2020，21（1）：270.
YANG W Q，LI S M，ZHAO Y，et al. Changes in the expression of interleukin-10 in myocardial infarction and its relationship with macrophage activation and cell apoptosis[J]. Ann Transl Med，2020，8（10）：643.
BOSHTAM M，ASGARY S，KOUHPAYEH S，et al. Aptamers against pro- and anti-inflammatory cytokines：a review[J]. Inflammation，2017，40（1）：340-349.
MARAFINI I，SEDDA S，DINALLO V，et al. Inflam- matory cytokines：from discoveries to therapies in IBD[J]. Expert Opin Biol Ther，2019，19（11）：1207-1217.
赵玉玺，魏莹，陈珍，等. 透明质酸修饰的绿原酸脂质体对U14宫颈癌荷瘤小鼠的抑制作用[J]. 现代生物医学进展，2017，17（26）：5029-5033.
ZHAO Y X，WEI Y，CHEN Z，et al. The anti tumor effect of hyaluronic acid decoreted chlorogenic acid liposomes on mice bearing uterine cervical carcinoma（U14）[J]. Prog Mod Biomed，2017，17（26）：5029-5033.
CHEN D，PAN D，TANG S，et al. Administration of chlorogenic acid alleviates spinal cord injury via TLR4/NF-κB and p38 signaling pathway anti-inflammatory activity[J]. Mol Med Rep，2018，17（1）：1340-1346.
ZHAI Q，LI F，CHEN X Y，et al. Triggering receptor expressed on myeloid cells 2，a novel regulator of immunocyte phenotypes，confers neuroprotection by relieving neuroinflammation[J]. Anesthesiology，2017，127（1）：98-110.
SHOU J Q，KONG X Z，WANG X Y，et al. Tizoxanide inhibits inflammation in LPS-activated RAW264.7 macrophages via the suppression of NF-κB and MAPK activation[J]. Inflammation，2019，42（4）：1336-1349.
XUE N N，ZHOU Q，JI M，et al. Chlorogenic acid inhibits glioblastoma growth through repolarizating macrophage from M2 to M1 phenotype[J]. Sci Rep，2017，7：39011.
KUMAR M K，SURESH M K，DALUS D. Macrophage activation syndrome[J]. J Assoc Physicians India，2006，54：238-240.
MURRAY P J，ALLEN J E，BISWAS S K，et al. Macrophage activation and polarization：nomenclature and experimental guidelines[J]. Immunity，2014，41（1）：14-20.
VOGEL D Y，GLIM J E，STAVENUITER A W，et al. Human macrophage polarization in vitro：maturation and activation methods compared[J]. Immunobiology，2014，219（9）：695-703.
YAN G L，CHEN L Y，WANG H H，et al. Baicalin inhibits LPS-induced inflammation in RAW264.7 cells through miR-181b/HMGB1/TRL4/NF-κB pathway[J]. Am J Transl Res，2021，13（9）：10127-10141.
FORD J W，MCVICAR D W. TREM and TREM-like receptors in inflammation and disease[J]. Curr Opin Immunol，2009，21（1）：38-46.
YE Y，SONG Y N，ZHUANG J H，et al. Anticancer effects of echinacoside in hepatocellular carcinoma mouse model and HepG2 cells[J]. J Cell Physiol，2019，234（2）：1880-1888.
SHARIF O，GAWISH R，WARSZAWSKA J M，et al. The triggering receptor expressed on myeloid cells 2 inhibits complement component 1q effector mechanisms and exerts detrimental effects during pneumococcal pneumonia[J]. PLoS Pathog，2014，10（6）：e1004167.
LI Y X，LIU X B，GUO L X，et al. Traditional Chinese herbal medicine for treating novel coronavirus （COVID-19） pneumonia：protocol for a systematic review and meta-analysis[J]. Syst Rev，2020，9（1）：75.
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