YANG Liping,ZHOU Shuai,JIA Qing,et al.Study on the mechanism of Chonghe paste promoting the dissipation of swollen lesions in vivo and in vitro[J].ZHONGGUO YAOFANG,2023,34(22):2708-2714.
YANG Liping,ZHOU Shuai,JIA Qing,et al.Study on the mechanism of Chonghe paste promoting the dissipation of swollen lesions in vivo and in vitro[J].ZHONGGUO YAOFANG,2023,34(22):2708-2714. DOI: 10.6039/j.issn.1001-0408.2023.22.04.
Study on the mechanism of Chonghe paste promoting the dissipation of swollen lesions in vivo and in vitro
To explore the mechanism of Chonghe paste promoting the dissipation of swollen lesions.
METHODS
2
The bacteriostatic effects of Chonghe paste against
Staphylococcus aureus
,
Escherichia coli
,
Pseudomonas aeruginosa
,
Staphylococcus albus
and
Streptococcus pneumoniae
were detected by punching method. The subcutaneous soft tissue infection model of rats was established by subcutaneous injection of
S. aureus
. The effects of 14 d intervention of Chonghe paste (Compound polymyxin B ointment as positive control) on the pathological changes of subcutaneous soft tissue, the protein expressions of type Ⅰ collagen, type Ⅲ collagen, matrix metalloproteinase-2 (MMP-2) and MMP-9 in subcutaneous soft tissue, and the contents of transforming growth factor-β (TGF-β) and basic fibroblast growth factor (bFGF) in serum were investigated.
RESULTS
2
Chonghe paste had varying degrees of bacteriostatic effect on the above 4 bacteria (except for
S. pneumoniae
), especially on
S. aureus.
Compared with the model group, on the 7th day of treatment, collagen fibers in the Chonghe paste group were arranged in an orderly manner, pus dissipated faster; the protein expressions of type Ⅰ and type Ⅲ collagen and the contents of TGF-β and bFGF were up-regulated significantly, while protein expressions of MMP-2 and MMP-9 were decreased significantly (
P
<0.05). On the 14th day of administration, collagen deposition was obvious in the Chonghe paste group, subcutaneous appendages gradually formed; the protein expressions of type Ⅰ and type Ⅲ collagen and the contents of TGF-β and bFGF were down-regulated significantly, while the protein expressions of MMP-2 and MMP-9 were increased significantly (
P
<0.05).
CONCLUSIONS
2
Chonghe paste has the bacteriostatic effect and may play a role in promoting the dissipation of swollen lesions by regulating the formation and decomposition of fibrin and increasing the secretion of bFGF and TGF-β.
关键词
冲和膏肿疡抑菌作用机制
Keywords
swollen lesionbacteriostasismechanism
references
陈红风. 中医外科学[M]. 10版. 北京:中国中医药出版社,2016:42-43.
CHEN H F. Traditional Chinese medicine surgery[M]. 10th ed. Beijing:China Press of Traditional Chinese Medicine,2016:42-43.
PAN H B,WANG T M,CUI Y,et al. Effects of Sanhuang ointment on subcutaneous soft tissue inflammation and TLR2/NF-κB signaling pathway in MRSA infection model rats[J]. Chin J Inf Tradit Chin Med,2022,29(8):54-59.
LIU Y,SHI Z Q,SHI J P. Understand the syndrome of half-Yin and half-Yang in surgery of TCM from Chonghe paste[J]. China J Tradit Chin Med Pharm,2020,35(7):3403-3405.
XIE J H. Effect evaluation of traditional Chinese medicine compound Chonghe ointment on acute mastitis[J]. Inn Mong J Tradit Chin Med,2022,41(4):115-117.
GE W J,CHEN X,DENG X M,et al. Analysis of therapeutic effect of Chonghe ointment on subcutaneous hematoma related to autologous arteriovenous fistula puncture[J]. J Extern Ther Tradit Chin Med,2021,30(4):13-15.
SHI J H,JI L T,LUO Q H,et al. Research progress on chemical constituents,pharmacological effects and quality markers prediction and analysis of Acorus gramineus[J]. Chin Tradit Pat Med,2021,43(5):1286-1290.
WANG R,LIU J,YANG D Y,et al. Research progress in chemical constituents and pharmacological action of Angelica dahurica[J]. Inf Tradit Chin Med,2020,37(2):123-128.
周璐丽. 独活化学成分及其抗菌、抗氧化活性研究[D]. 长沙:湖南农业大学,2020.
ZHOU L L. Studies on chemical composition and antimicrobic and antioxidant activities of Angelica pubescens[D]. Changsha:Hunan Agricultural University,2020.
YANG Y H,XU X J,LI C X,et al. New progress in research on chemical constituents and pharmacological action of Radix Paeoniae Rubra[J]. Chem Eng,2021,35(9):42-44,31.
LI Z H,ZHENG Z T,LIANG S L. Screening of the analgesic and anti-inflammatory active fractions from Cercis chinensis bunge bark[J]. Drug Eval,2023,20(4):402-407.
JIANG W,ZOU G F,GU C M,et al. Establishment of skin abscess model in mice and pharmacodynamic evalu-ation of Pouzolzia zeylanica var. microphylla[J]. Her Med,2020,39(3):298-304.
GUO G M,FAN Z,SHI L,et al. Clinical study on treatment of acute oncotic ulcer by the external application of Furong ointment[J]. World Chin Med,2016,11(12):2689-2691.
QIN T Y,MA S L,HE J. Discrimination of the pathogene-sis of Yong(carbuncle)and Zhong(swelling)in Huangdi Neijing[J]. J Beijing Univ Tradit Chin Med,2021,44(11):982-986.
WANG J,LIU S,DAI M,et al. Theoretical connotation and application of the method of elimination and suppor-ting from the perspective of external treatment to expel pathogenic factors[J]. China J Tradit Chin Med Pharm,2022,37(2):764-767.
JIANG Y,FENG G F,HUANG J L,et al. Application of encircling paste in the green treatment of tumor:based on the ancient idea of “a siege must be missed”[J]. Jilin J Chin Med,2021,41(2):144-146.
ZHU Y,WU S L. Forty-eight cases of hyperplasia of mammary glands with intermingled phlegm and blood stasis syndrome treated with decoction for activating blood circulation and removing phlegm and dispersing stagnation in combination with chonghe paste[J]. Henan Tradit Chin Med,2018,38(11):1710-1714.
HE X W,FENG L. Effect of microwave therapy on postoperative serological indexes and clinical efficacy in patients with hemorrhoids and fistula[J]. Chin J Health Care Med,2023,25(3):360-362.
WEN L H,FENG J X,CHEN X Q,et al. Combined Maoshi thunder-fire moxibustion with Qudu xiaozhong decoction on postoperative pain,edema and wound hea-ling of mixed hemorrhoids of damp heat type[J]. Hebei J Tradit Chin Med,2022,44(12):1973-1978.
MA S H,SUN Y N,YANG W F,et al. Repair effect and mechanism of recombinant human collagen on laser induced damage of mice skin[J]. Acta Laser Biology Sinica,2018,27(5):399-406.
YU H Z,ZHENG X,LU L H,et al. The effect of tissue kallikrein 1 on macrophage migration inhibitory factor and matrix metalloproteinases expression in rat with myocardial infarction[J]. Chin J Hypertens,2021,29(12):1258-1265.
YANG C Y,LIU Y,LI Y,et al. Effects of compound hibiscus flower and leaf extract on wound healing in rats with post-burn trauma infection and its mechanism of action[J]. Chin J Nosocomiology,2023,33(12):1783-1787.