Study on the fingerprint of total saponins from <italic style="font-style: italic">Mussaenda pubescens</italic> and the spectrum-effect relationship of its hepatoprotective activity

ZHANG Ying; LUO Jinrong; ZHANG Yunyun; CHEN Feng; HE Fei; LI Jia

doi:10.6039/j.issn.1001-0408.2023.22.05

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Study on the fingerprint of total saponins from Mussaenda pubescens and the spectrum-effect relationship of its hepatoprotective activity

更新时间：2023-11-25

- Study on the fingerprint of total saponins from Mussaenda pubescens and the spectrum-effect relationship of its hepatoprotective activity
- ZHONGGUO YAOFANG Vol. 34, Issue 22, Pages: 2715-2720(2023)
- 作者机构：
  
  1.广西壮族自治区中医药研究院，南宁　530022
  2.广西中药质量标准研究重点实验室，南宁　530022
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.22.05
  CLC： R917;R965
- Published：30 November 2023，
  
  Received：10 May 2023，
  
  Revised：01 September 2023，
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张颖,罗金荣,张赟赟等.玉叶金花总皂苷指纹图谱及其肝保护作用的谱效关系研究 ^Δ[J].中国药房,2023,34(22):2715-2720.

ZHANG Ying,LUO Jinrong,ZHANG Yunyun,et al.Study on the fingerprint of total saponins from Mussaenda pubescens and the spectrum-effect relationship of its hepatoprotective activity[J].ZHONGGUO YAOFANG,2023,34(22):2715-2720.
张颖,罗金荣,张赟赟等.玉叶金花总皂苷指纹图谱及其肝保护作用的谱效关系研究 ^Δ[J].中国药房,2023,34(22):2715-2720. DOI： 10.6039/j.issn.1001-0408.2023.22.05.

ZHANG Ying,LUO Jinrong,ZHANG Yunyun,et al.Study on the fingerprint of total saponins from Mussaenda pubescens and the spectrum-effect relationship of its hepatoprotective activity[J].ZHONGGUO YAOFANG,2023,34(22):2715-2720. DOI： 10.6039/j.issn.1001-0408.2023.22.05.

摘要

目的

建立玉叶金花总皂苷指纹图谱，并进行其肝保护作用的谱效关系研究。

方法

取不同产地的玉叶金花药材，以75%乙醇为溶剂，制备10批玉叶金花总皂苷。采用高效液相色谱法测定，利用《中药色谱指纹图谱相似度评价系统（2012版）》绘制10批玉叶金花总皂苷的指纹图谱，并进行相似度评价、共有峰指认。采用同一高效液相色谱法，测定其中5种三萜皂苷类成分（mussaendoside H、mussaendoside U、mussaglaoside C、mussaendoside G、mussaendoside O）的含量。以四氯化碳致急性肝损伤小鼠模型考察玉叶金花总皂苷的肝保护作用，并通过灰色关联度分析法进行谱效关系研究。

结果

10批玉叶金花总皂苷共有11个共有峰，指认了其中5个，分别为mussaendoside H（3号峰）、mussaendoside U（7号峰）、mussaglaoside C（8号峰）、mussaendoside G（9号峰）、mussaendoside O（11号峰）；10批样品的相似度为0.940～0.991。mussaendoside H、mussaendoside U、mussaglaoside C、mussaendoside G、mussaendoside O的平均含量分别为0.01～0.05、0.10～0.21、0.10～0.18、0.03～0.08、0.20～0.40 mg/g。10批玉叶金花总皂苷可普遍降低模型小鼠血清中丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）含量和肝组织中肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）、IL-1β含量（

＜0.05或

＜0.01）。各共有峰峰面积与ALT、AST、TNF-α、IL-6、IL-1β含量的关联度分别为0.602～0.757、0.585～0.833、0.593～0.795、0.618～0.820、0.607～0.804，关联度均较大的依次为11、9、8号峰。

结论

10批玉叶金花总皂苷成分相似，mussaendoside H、mussaendoside U、mussaglaoside C、mussaendoside G、mussaendoside O的平均含量不一；10批样品均有一定的肝保护作用，mussaendoside O、mussaendoside G、mussaglaoside C可能是其主要药效成分。

Abstract

OBJECTIVE

To establish the fingerprint of total saponins from

Mussaenda pubescens

， and to study the spectrum-effect relationship of its hepatoprotective activity.

METHODS

Ten batches of total saponins from

M. pubescens

from different origins were prepared using 75% ethanol as solvent. High-performance liquid chromatography （HPLC） and the

Similarity Evaluation System for Traditional Chinese Medicine Chromatographic Fingerprints

（2012 edition） were used to draw the fingerprints of 10 batches of total saponins from

M. pubescens

. The similarity evaluation and identification of common peaks were conducted. The same HPLC method was adopted to determine the contents of five triterpenoid saponins （mussaendoside H， mussaendoside U， mussaglaoside C， mussaendoside G and mussaendoside O）. The hepatoprotective effect of total saponins from

M. pubescens

was investigated by establishing carbon tetrachloride-induced acute liver injury model mice， and the spectrum-effect relationship was studied by using grey correlation analysis.

RESULTS

There were 11 common peaks in 10 batches of total saponins from

M. pubescens

， 5 of which were identified， i.e. mussaendoside H （peak 3）， mussaendoside U （peak 7）， mussaglaoside C （peak 8）， mussaendoside G （peak 9） and mussaendoside O （peak 11）； the similarities of 10 batches of samples ranged 0.940-0.991. Average contents of mussaendoside H， mussaendoside U， mussaglaoside C， mussaendoside G， mussaendoside O were 0.01-0.05， 0.10-0.21， 0.10-0.18， 0.03-0.08， 0.20-0.40 mg/g， respectively. Ten batches of total saponins from

M. pubescens

could generally reduce the contents of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） in serum， and tumor necrosis factor-α （TNF-α）， interleukin-6 （IL-6） and IL-1β in liver tissue of model mice （

＜0.05 or

＜0.01）. The correlation between the common peak areas and the contents of ALT， AST， TNF-α， IL-6 and IL-1β were 0.602-0.757， 0.585-0.833， 0.593-0.795， 0.618-0.820， 0.607-0.804， respectively； the peaks with high correlation were peaks 11， 9 and 8 in order.

CONCLUSIONS

Ten batches of total saponins from

M. pubescens

have similar components， and the average contents of mussaendoside H， mussaendoside U， mussaglaoside C， mussaendoside G and mussaendoside O are different. The batches of samples have a certain degree of hepatoprotective effect； mussaendoside O， mussaendoside G and mussaglaoside C may be its main active components.

关键词

玉叶金花总皂苷指纹图谱含量测定肝保护作用谱效关系

Keywords

total saponinsfingerprintcontent determinationhepatoprotective activityspectrum-effect relationship

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Study on the fingerprint of total saponins from Mussaenda pubescens and the spectrum-effect relationship of its hepatoprotective activity

DOI：10.6039/j.issn.1001-0408.2023.22.05

摘要

Abstract

关键词

Keywords

references