CHEN Xiaokai,LI Yong,TAN Wei,et al.Establishment of HPLC fingerprint and content determination of 8 components in Jianpi huayu decoction[J].ZHONGGUO YAOFANG,2023,34(23):2874-2879.
To establish the HPLC fingerprint of Jianpi huayu decoction, and to determine the contents of 8 components.
METHODS
2
Thermo Hypersil Gold C
18
column was used with mobile phase consisted of methanol-0.05% phosphoric acid aqueous solution (gradient elution) at the flow rate of 1.0 mL/min. The column temperature was 30 ℃, the injection volume was 5 μL. The detection wavelength of matrine was 211 nm, and the other components’ detection wavelength was 283 nm. The similarity evaluation of HPLC fingerprints for 10 batches of Jianpi huayu decoction was performed by using
the
Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine
(2012 edition). The contents of chlorogenic acid, vanillic acid, p-coumaric acid, ferulic acid, hesperidin, quercetin, bergapten and matrine in the samples were determined by HPLC.
RESULTS
2
HPLC fingerprint of Jianpi huayu decoction was established. A total of 27 common peaks were identified, and 8 components were identified. The similarity between 10 batches of samples and the control map ranged from 0.942-0.999. RSDs of precision, repeatability and stability tests were less than 3% (
n
=6). The average recoveries of chlorogenic acid, vanillic acid, p-coumaric acid, ferulic acid, hesperidin, quercetin, bergapten and matrine were 99.48%, 101.32%, 101.18%, 100.79%, 101.12%, 99.19%, 99.81% and 102.46%, respectively; RSDs were 1.34%, 0.93%, 1.90%, 1.84%, 0.54%, 1.53%, 1.33% and 1.01%, respectively (
n
=6). The contents were 0.021-0.061, 0.025-0.034, 0.116-0.295, 0.006-0.062, 0.014-0.053, 0.017-0.026, 0.014-0.027 and 14.05-24.11 mg/g, respectively.
CONCLUSIONS
2
The established fingerprint and content determination method can provide a reference for the quality control and subsequent preparation development for Jianpi huayu decoction.
关键词
健脾化瘀方指纹图谱含量测定高效液相色谱法质量标准
Keywords
fingerprintcontent determinationHPLCquality standard
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