浏览全部资源
扫码关注微信
1.云南中医药大学中药学院,昆明 650500
2.昆明医科大学第一附属医院临床药学中心,昆明 650032
Published:15 December 2023,
Received:22 April 2023,
Revised:19 August 2023,
扫 描 看 全 文
王锦,夏霜莉,杨媛等.天麻活性成分减轻大鼠神经元氧糖剥夺/复糖复氧损伤的作用机制 Δ[J].中国药房,2023,34(23):2886-2890.
WANG Jin,XIA Shuangli,YANG Yuan,et al.Mechanism of Gastrodia elata active ingredient reducing oxygen-glucose deprivation/reoxygenation injury in rat neuron[J].ZHONGGUO YAOFANG,2023,34(23):2886-2890.
王锦,夏霜莉,杨媛等.天麻活性成分减轻大鼠神经元氧糖剥夺/复糖复氧损伤的作用机制 Δ[J].中国药房,2023,34(23):2886-2890. DOI: 10.6039/j.issn.1001-0408.2023.23.12.
WANG Jin,XIA Shuangli,YANG Yuan,et al.Mechanism of Gastrodia elata active ingredient reducing oxygen-glucose deprivation/reoxygenation injury in rat neuron[J].ZHONGGUO YAOFANG,2023,34(23):2886-2890. DOI: 10.6039/j.issn.1001-0408.2023.23.12.
目的
2
研究天麻活性成分3,4-二羟基苯甲醛(3,4-DD)对大鼠脑微血管内皮细胞(BMECs)-大鼠肾上腺嗜铬细胞PC12共培养体系氧糖剥夺/复糖复氧(OGD/R)损伤的改善作用机制。
方法
2
采用Transwell小室共培养BMECs与PC12细胞,然后分为对照组、模型组、丁苯酞组(阳性对照组,0.1 mmol/L)、3,4-DD组(0.1 μmol/L),除对照组外,其余各组共培养体系均复制OGD/R损伤模型。同时,共培养体系以相应药物或培养基干预BMECs 24 h,然后检测体系跨膜电阻(TEER)以及PC12细胞中乳酸脱氢酶(LDH)活性、脑源性神经营养因子(BDNF)水平以及TrkB、Plc-γ、Map-2、GAP-43 mRNA表达水平。
结果
2
与对照组比较,模型组共培养体系TEER以及PC12细胞中LDH活性、BDNF水平均显著降低(
P
<0.01),PC12细胞中TrkB、Plc-γ、Map-2、GAP-43 mRNA表达水平均显著升高(
P
<0.01);与模型组比较,3,4-DD组、丁苯酞组共培养体系TEER以及PC12细胞中LDH活性、BDNF水平和TrkB、Plc-γ、Map-2、GAP-43 mRNA表达水平均显著升高(
P
<0.05或
P
<0.01)。
结论
2
3,4-DD可通过作用于BMECs减轻神经元OGD/R损伤,其作用机制可能与激活BDNF/TrkB信号通路有关。
OBJECTIVE
2
To study the improvement effect and mechanism of
Gastrodia elata
active ingredient 3,4-dihydroxybenzaldehyde (3,4-DD) on oxygen-glucose deprivation/reoxygenation(OGD/R) injury in rat primary brain microvascular endothelial cells (BMECs)-rat adrenal chromaffin cells PC12 co-culture system.
METHODS
2
The co-culture model of BMECs and PC12 cells was replicated in the Transwell chamber, and divided into control group, model group, butylphthalide group (positive control group, 0.1 mmol/L) and 3,4-DD group (0.1 μmol/L). OGD/R injury model of the co-culture system was induced in those groups except for the control group. After preventively intervention in BMECs with relevant medicine or culture medium for 24 h, cell transendothelial electronic resistance (TEER) value, lactate dehydrogenase (LDH) activity, brain-derived neurotrophic factor (BDNF) level and mRNA expressions of TrkB, Plc-γ, Map-2, GAP-43 in PC12 cells was detected.
RESULTS
2
Compared with the control group, TEER of the co-culture model, LDH activity and BDNF level of PC12 cells were decreased significantly in the model group (
P
<0.01), while mRNA expressions of TrkB, Plc-γ, Map-2 and GAP-43 in PC12 cells were increased significantly (
P
<0.01). Compared with the model group, TEER of the co-culture model, LDH activity, BDNF level, and the mRNA expressions of TrkB, Plc-γ, Map-2 and GAP-43 in PC12 cells were increased significantly in the 3,4-DD group and butylphthalide group (
P
<0.05 or
P
<0.01).
CONCLUSIONS
2
3,4-DD can relieve the damage of neuronal OGD/R by acting on BMECs, the mechanism of which may be associated with activating the BDNF/TrkB signaling pathway.
3,4-二羟基苯甲醛脑微血管内皮细胞神经元氧糖剥夺
brain microvascular endothelial cellsneuronoxygen and glucose deprivation
刘抒雯,杨丽华,马春,等. 中医药保护脑缺血再灌注损伤后神经血管单元的作用[J]. 中国实验方剂学杂志,2018,24(23):225-234.
LIU S W,YANG L H,MA C,et al. Effect of neurovascular unit after cerebral ischemia-reperfusion injury treated by traditional Chinese medicine[J]. Chin J Exp Tradit Med Formulae,2018,24(23):225-234.
LE R,ESQUENAZI S. Astrocytes mediate cerebral cortical neuronal axon and dendrite growth,in part,by release of fibroblast growth factor[J]. Neurol Res,2002,24(1):81-92.
夏霜莉,杨媛,廖陈,等. 3,4-二羟基苯甲醛作用星形胶质细胞减轻脑微血管内皮细胞和神经元氧糖剥夺/再灌注损伤[J]. 上海中医药大学学报,2021,35(4):67-72,100.
XIA S L,YANG Y,LIAO C,et al. 3,4-Dihydroxybenzaldehyde attenuates oxygen-glucose deprivation/reperfusion injury in brain microvascular endothelial cells and neurons by acting on astrocytes[J]. Acad J Shanghai Univ Tradit Chin Med,2021,35(4):67-72,100.
吴雄枫,袁绘,沈红健,等. 静脉溶栓治疗反复短暂性脑缺血发作的疗效分析[J]. 第二军医大学学报,2022,43(1):55-59.
WU X F,YUAN H,SHEN H J,et al. Efficacy of intravenous thrombolysis for treatment of repeated transient is- chemic attack[J]. Acad J Second Mil Med Univ,2022,43(1):55-59.
何芳雁. 天麻对MCAO/R模型大鼠血脑屏障的保护作用及机制研究[D]. 昆明:云南中医学院,2015.
HE F Y. Protective effect and mechanisms of the Gastrodia elata Blume. on the blood-brain barrier of MCAO/R model rats[D]. Kunming:Yunnan University of Traditional Chinese Medicine,2015.
颜汉文. 天麻酚性成分对神经血管单元的保护作用[D]. 昆明:云南中医学院,2017.
YAN H W. Protective effects of the phenolic component from Gastrodia elata Blume. on the neurovascular unit[D]. Kunming:Yunnan University of Traditional Chinese Medicine,2017.
吴盛友. 天麻酚性成分对PC12细胞及胎鼠皮层神经元OGD/R损伤保护作用的研究[D]. 昆明:云南中医学院,2013.
WU S Y. Protective effects of phenols from Gastrodia elata Blume on PC12 cell and cortical neurons of fetal rats after OGD/R[D]. Kunming:Yunnan University of Traditional Chinese Medicine,2013.
李长香. 精制清开灵及其组分对体外神经血管单元的保护作用及机制的研究[D]. 北京:北京中医药大学,2019.
LI C X. Study on the protective effect and mechanism of refined Qingkailing and its components on neurovascular units in vitro[D].Beijing:Beijing University of Chinese Medicine,2019.
APPELT-MENZEL A,CUBUKOVA A,GÜNTHER K,et al. Establishment of a human blood-brain barrier co-culture model mimicking the neurovascular unit using induced pluri- and multipotent stem cells[J]. Stem Cell Reports,2017,8(4):894-906.
薛强. “脑神经血管单元” 体外模型的成模性研究[D]. 重庆:西南大学,2013.
XUE Q. The study of the brain neurovascular unit model in vitro[D]. Chongqing:Southwest University,2013.
李静. 丁苯酞活化AMPK调控线粒体生成对脑缺血/再灌注损伤的保护作用[D]. 沈阳:中国医科大学,2022.
LI J. Protective effect of mitochondrial biogenesis regulated by butylphthalide activating AMPK in cerebral is- chemia/reperfusion injury[D]. Shenyang:China Medical University,2022.
LIU F D,DUAN H M,HAO F,et al. Biomimetic chitosan scaffolds with long-term controlled release of nerve growth factor repairs 20-mm-long sciatic nerve defects in rats[J]. Neural Regen Res,2022,17(5):1146-1155.
NAKAGAWA S,DELI M A,NAKAO S,et al. Pericytes from brain microvessels strengthen the barrier integrity in primary cultures of rat brain endothelial cells[J]. Cell Mol Neurobiol,2007,27(6):687-694.
KUMAR P,NAGARAJAN A,UCHIL P D. Analysis of cell viability by the lactate dehydrogenase assay[J]. Cold Spring Harb Protoc,2018,2018(6):465-468.
KISS BIMBOVA K,BACOVA M,KISUCKA A,et al. Activation of three major signaling pathways after endurance training and spinal cord injury[J]. Mol Neurobiol,2022,59(2):950-967.
YOONJU K,YOU-NA J,JI-YOUNG K,et al. Microtubule-associated protein 2 mediates induction of long-term potentiation in hippocampal neurons[J]. FASEB J Off Publ Fed Am Soc Exp Biol,2020,34(5):6965-6983.
LIU D,LIU Z,LIU H X,et al. Brain-derived neurotrophic factor promotes vesicular glutamate transporter 3 expression and neurite outgrowth of dorsal root ganglion neurons through the activation of the transcription factors Etv4 and Etv5[J]. Brain Res Bull,2016,121:215-226.
LEAL G,COMPRIDO D,DUARTE C B. BDNF-induced local protein synthesis and synaptic plasticity[J]. Neuropharmacology,2014,76:639-656.
0
Views
1
下载量
0
CSCD
Publicity Resources
Related Articles
Related Author
Related Institution