Effects and mechanism of anaphylactoid reaction induced by nonapeptide activating mast cells

CHEN Xiaomin; LIU Na; ZHANG Lina; LI Youjia

doi:10.6039/j.issn.1001-0408.2023.24.07

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Effects and mechanism of anaphylactoid reaction induced by nonapeptide activating mast cells

更新时间：2023-12-22

- Effects and mechanism of anaphylactoid reaction induced by nonapeptide activating mast cells
- ZHONGGUO YAOFANG Vol. 34, Issue 24, Pages: 2995-2999(2023)
- 作者机构：
  
  西安交通大学第二附属医院药学部，西安　710004
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.24.07
  CLC： R965
- Published：30 December 2023，
  
  Received：04 July 2023，
  
  Revised：24 October 2023，
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陈晓敏,刘娜,张丽娜等.九肽激活肥大细胞致类过敏反应的作用及其机制 ^Δ[J].中国药房,2023,34(24):2995-2999.

CHEN Xiaomin,LIU Na,ZHANG Lina,et al.Effects and mechanism of anaphylactoid reaction induced by nonapeptide activating mast cells[J].ZHONGGUO YAOFANG,2023,34(24):2995-2999.
陈晓敏,刘娜,张丽娜等.九肽激活肥大细胞致类过敏反应的作用及其机制 ^Δ[J].中国药房,2023,34(24):2995-2999. DOI： 10.6039/j.issn.1001-0408.2023.24.07.

CHEN Xiaomin,LIU Na,ZHANG Lina,et al.Effects and mechanism of anaphylactoid reaction induced by nonapeptide activating mast cells[J].ZHONGGUO YAOFANG,2023,34(24):2995-2999. DOI： 10.6039/j.issn.1001-0408.2023.24.07.

摘要

目的

探讨九肽IVQKIKHCF激活肥大细胞引发类过敏反应的作用及潜在机制。

方法

以人肥大细胞系LAD2为对象，以P物质为阳性对照，通过检测

-氨基己糖苷酶释放率、组胺释放量、炎症因子释放量来考察25、50、100 μmol/L IVQKIKHCF的激活作用；分别以敲低

MrgprX2

的LAD2细胞和Mas相关G蛋白偶联受体X2（MRGPRX2）高表达的人胚胎肾细胞系HEK293（MRGPRX2/HEK293细胞）为对象，通过检测

-氨基己糖苷酶释放率和细胞内钙离子浓度来评价IVQKIKHCF激活作用与MRGPRX2的相关性。

结果

25、50、100 μmol/L的IVQKIKHCF可显著提高LAD2细胞的

-氨基己糖苷酶释放率和组胺释放量（

＜0.05），并不同程度地促进肿瘤坏死因子α、白细胞介素8、巨噬细胞炎症蛋白1β、单核细胞趋化蛋白1的释放（

＜0.05）；敲低

MrgprX2

后，25、50、100 μmol/L的IVQKIKHCF对LAD2细胞

-氨基己糖苷酶释放的促进作用被显著逆转（

＜0.05），且可升高MRGPRX2/HEK293细胞内的钙离子浓度。

结论

九肽IVQKIKHCF可通过激活MRGPRX2而促进肥大细胞释放颗粒物质和炎症介质，从而引发类过敏反应。

Abstract

OBJECTIVE

To study the effects and potential mechanism of anaphylactoid reaction induced by nonapeptide IVQKIKHCF activating mast cells.

METHODS

Using human mast cell line LAD2 as subject， and substance P as positive control， the activation effects of 25， 50 and 100 μmol/L IVQKIKHCF on mast cells were investigated by determining the release rate of

-aminohexosidase， histamine release， and the contents of inflammatory factors； using

MrgprX2

-knockdown LAD2 cells and Mas-related G protein-coupled receptor X2 （MRGPRX2） high-expression human embryonic kidney cell line HEK293 （MRGPRX2/HEK293 cells） as subject， the correlation between the activation effect of IVQKIKHCF and MRGPRX2 was investigated by determining the release rate of

-aminohexosidase， and intracellular calcium ion concentration.

RESULTS

IVQKIKHCF with 25， 50， 100 μmol/L could significantly increase the release rate of

-aminohexosidase and histamine release in LAD2 cells （

＜0.05）， and promote the release of tumor necrosis factor-α， interleukin-8， macrophage inflammatory protein-1β and monocyte chemotactic protein-1 to varying degrees （

＜0.05）. After knocking down

MrgprX2

， the effects of 25， 50， 100 μmol/L IVQKIKHCF promoting the release of

-aminohexosidase in LAD2 cells were reversed significantly （

＜0.05）， resulting in an increase of calcium ion concentration in MRGPRX2/HEK293 cells.

CONCLUSIONS

Nonapeptide IVQKIKHCF can promote mast cells to release granular matter and inflammatory mediators by activating MRGPRX2 thus inducing anaphylactoid reaction.

关键词

九肽IVQKIKHCF肥大细胞类过敏反应Mas相关G蛋白偶联受体X2

Keywords

IVQKIKHCFmast cellanaphylactoid reactionMas-related G protein-coupled receptor X2

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