Method establishment of component identification and content determination of 7 components in Mongolian medicine <italic style="font-style: italic">Ixeris chinensis</italic>

SUN Lijun; LI Jun; WANG Qiutong; XIA Huimin; WANG Yuewu; ZHANG Qian; ZHANG Huiwen; WANG Huanyun

doi:10.6039/j.issn.1001-0408.2023.24.09

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Method establishment of component identification and content determination of 7 components in Mongolian medicine Ixeris chinensis

更新时间：2023-12-22

- Method establishment of component identification and content determination of 7 components in Mongolian medicine Ixeris chinensis
- ZHONGGUO YAOFANG Vol. 34, Issue 24, Pages: 3006-3011(2023)
- 作者机构：
  
  1.内蒙古医科大学药学院，呼和浩特　010110
  2.内蒙古自治区新药筛选工程研究中心，呼和浩特　010110
  3.内蒙古医科大学附属医院药物临床试验机构，呼和浩特　010110
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.24.09
  CLC： R917
- Published：30 December 2023，
  
  Received：03 July 2023，
  
  Revised：16 November 2023，
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孙丽君,李君,王秋桐等.蒙药材山苦荬的成分鉴别及7种成分含量测定方法建立 ^Δ[J].中国药房,2023,34(24):3006-3011.

SUN Lijun,LI Jun,WANG Qiutong,et al.Method establishment of component identification and content determination of 7 components in Mongolian medicine Ixeris chinensis[J].ZHONGGUO YAOFANG,2023,34(24):3006-3011.
孙丽君,李君,王秋桐等.蒙药材山苦荬的成分鉴别及7种成分含量测定方法建立 ^Δ[J].中国药房,2023,34(24):3006-3011. DOI： 10.6039/j.issn.1001-0408.2023.24.09.

SUN Lijun,LI Jun,WANG Qiutong,et al.Method establishment of component identification and content determination of 7 components in Mongolian medicine Ixeris chinensis[J].ZHONGGUO YAOFANG,2023,34(24):3006-3011. DOI： 10.6039/j.issn.1001-0408.2023.24.09.

摘要

目的

建立鉴别山苦荬的化学成分以及7种成分（绿原酸、木犀草素、槲皮素、芦丁、原儿茶酸、异绿原酸A、木犀草苷）的含量测定方法。

方法

采用高效液相色谱-四极杆/静电场轨道阱高分辨质谱技术对山苦荬的化学成分进行鉴定，采用高效液相色谱串联三重四极杆质谱技术测定山苦荬中绿原酸等7种成分的含量。

结果

从山苦荬药材中共鉴别出45个成分，包括20个有机酸类成分、13个黄酮类成分、4个脂肪酸类成分、4个氨基酸成分、3个核苷类成分和1个香豆素类成分。绿原酸、木犀草素、槲皮素、芦丁、原儿茶酸、异绿原酸A、木犀草苷检测质量浓度的线性范围分别为503.00～25 150.00、42.00～2 100.00、5.05～252.50、20.05～1 002.50、25.10～1 255.00、750.00～37 500.00、196.00～9 800.00 ng/mL（

≥0.999 2），精密度、稳定性、重复性试验的RSD均小于3.00%（

＝6），平均加样回收率为96.72%～105.84%（RSD均小于4.00%，

＝6）。3批山苦荬中绿原酸等7种成分的含量范围分别为1 145.77～3 261.25、23.75～97.90、0.92～2.12、1.06～23.18、9.35～21.85、833.25～1 045.58、199.56～1 869.78 μg/g。

结论

本研究所建立的鉴别和定量方法快速、简便，可用于山苦荬药材化学成分的鉴别和绿原酸等7种成分的含量测定。

Abstract

OBJECTIVE

To establish the methods to identify the chemical components of

Ixeris chinensis

， and determine the contents of 7 components （chlorogenic acid， luteolin， quercetin， rutin， protocatechuic acid， isochlorogenic acid A， luteoloside）.

METHODS

HPLC-Q-Exactive-MS was used to identify the chemical components of

I. chinensis

. The contents of 7 components in

I. chinensis

， including chlorogenic acid，

were determined by HPLC-MS/MS.

RESULTS

A total of 45 components were identified in

I. chinensis

， including 20 organic acids， 13 flavonoids， 4 fatty acids， 4 amino acids， 3 nucleosides， and 1 coumarin. The linear range of chlorogenic acid， luteolin， quercetin， rutin， protocatechuic acid， isochlorogenic acid A and luteoloside were 503.00- 25 150.00， 42.00-2 100.00， 5.05-252.50， 20.05-1 002.50， 25.10-1 255.00， 750.00-37 500.00， 196.00-9 800.00 ng/mL （

≥0.999 2）， respectively. RSDs of precision， stability and reproducibility tests were all less than 3.00% （

＝6）， and average recovery ranged from 96.72% to 105.84% （all RSD＜4.00%，

＝6）. The contents of 7 components in 3 batches of

I. chinensis

were 1 145.77- 3 261.25， 23.75-97.90， 0.92-2.12， 1.06-23.18， 9.35-21.85， 833.25-1 045.58， 199.56-1 869.78 μg/g， respectively.

CONCLUSIONS

The established methods for identification and content determination are rapid and simple， and can be used for the identification of chemical components and the content determination of 7 components in

hinensis

关键词

山苦荬高效液相色谱-四极杆/静电场轨道阱高分辨质谱技术高效液相色谱串联三重四极杆质谱技术成分鉴别含量测定

Keywords

HPLC-Q-Exactive-MSHPLC-MS/MScomponent identificationcontent determination

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Method establishment of component identification and content determination of 7 components in Mongolian medicine Ixeris chinensis

DOI：10.6039/j.issn.1001-0408.2023.24.09

摘要

Abstract

关键词

Keywords

references