Study on quality control method of the roots and rhizoma of <italic style="font-style: italic">Toricellia angulata</italic>

LI Xue; NIE Yushan; MA Xue; LU Yuan; YANG Chang; LI Yongjun; WANG Yonglin

doi:10.6039/j.issn.1001-0408.2024.01.04

您当前的位置：

首页 >

文章列表页 >

Study on quality control method of the roots and rhizoma of Toricellia angulata

更新时间：2024-01-11

- Study on quality control method of the roots and rhizoma of Toricellia angulata
- ZHONGGUO YAOFANG Vol. 35, Issue 1, Pages: 21-26(2024)
- 作者机构：
  
  1.贵州医科大学民族药与中药开发应用教育部工程研究中心/省部共建药用植物功效与利用国家重点实验室，贵阳　550004
  2.贵州医科大学药学院，贵阳　550004
  3.贵州医科大学贵州省药物制剂重点实验室，贵阳　550004
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.01.04
  CLC： R284;R917
- Published：15 January 2024，
  
  Received：18 July 2023，
  
  Revised：12 November 2023，
扫描看全文
黎雪,聂雨杉,马雪等.水冬瓜根及茎的质量控制方法研究 ^Δ[J].中国药房,2024,35(01):21-26.

LI Xue,NIE Yushan,MA Xue,et al.Study on quality control method of the roots and rhizoma of Toricellia angulata[J].ZHONGGUO YAOFANG,2024,35(01):21-26.
黎雪,聂雨杉,马雪等.水冬瓜根及茎的质量控制方法研究 ^Δ[J].中国药房,2024,35(01):21-26. DOI： 10.6039/j.issn.1001-0408.2024.01.04.

LI Xue,NIE Yushan,MA Xue,et al.Study on quality control method of the roots and rhizoma of Toricellia angulata[J].ZHONGGUO YAOFANG,2024,35(01):21-26. DOI： 10.6039/j.issn.1001-0408.2024.01.04.

摘要

目的

建立水冬瓜根及茎药材的质量控制方法。

方法

观察水冬瓜根及茎药材的性状、显微特征；按2020年版《中国药典》（四部）通则方法对水冬瓜根及茎药材中的水分、总灰分、酸不溶性灰分和醇溶性浸出物进行测定；采用《中药色谱指纹图谱相似度评价系统（2012版）》建立11批水冬瓜根及茎药材的高效液相色谱（HPLC）指纹图谱，并进行共有峰的指认及相似度评价；采用HPLC法测定水冬瓜根及茎中松柏苷、紫丁香苷、绿原酸、（+）-syringaresinol-

O-β-

D-glucopyranoside、丁香脂素的含量。

结果

水冬瓜根及茎药材性状与显微鉴别特征明显。水分、总灰分、酸不溶性灰分和醇溶性浸出物的含量平均值分别为7.54%、2.18%、0.15%、7.81%。11批水冬瓜根及茎药材的指纹图谱有16个共有峰，相似度为0.856～0.960，共指认出松柏苷、紫丁香苷、绿原酸、（+）-syringaresinol-

O-β-

D-glucopyranoside、丁香脂素5个共有成分。上述5个成分的含量分别为0.047 2～0.401 6、0.836 8～8.697 9、1.245 3～10.950 0、0.139 0～0.437 8、0.016 4～0.635 3 mg/g。

结论

所建方法稳定、准确性好，可用于水冬瓜根及茎药材的质量控制。初步拟定水冬瓜根及茎药材中水分不得过11.0%，总灰分不得过4.0%，醇溶性浸出物不得少于5.0%，松柏苷、紫丁香苷、绿原酸、（+）-syringaresinol-

O-β-

D-glucopyranoside、丁香脂素含量分别不得少于0.04、0.83、1.24、0.13、0.01 mg/g。

Abstract

OBJECTIVE

To establish the quality control method for the roots and rhizoma of

Toricellia angulata

METHODS

The properties of the roots and rhizoma of

T. angulata

were observed and microscopic identification was conducted. The moisture， total ash， acid-insoluble ash and ethanol-soluble extract were examined according to the method stated in the 2020 edition of

Chinese Pharmacopoeia

（part Ⅳ）. HPLC fingerprints of 11 batches of the roots and rhizoma of

T. angulata

were established， common peaks were identified and the similarity was evaluated by using the

Similarity Evaluation System of Chromatographic Fingerprint of TCM

（2012 edition）. The contents of coniferin， syringin， chlorogenic acid，（+）-syringaresinol-

-D-glucopyranoside and syringaresinol were determined by HPLC.

RESULTS

The properties and microscopic identification of the roots and rhizoma of

T. angulata

were obvious. The average contents of moisture， total ash， acid-insoluble ash and ethanol-soluble extract were 7.54%， 2.18%， 0.15% and 7.81%， respectively. There were 16 common peaks marked in the HPLC fingerprints of 11 batches of the roots and rhizoma of

T. angulata

， with similarities of 0.856-0.960； five of them were identified， such as coniferin， syringin， chlorogenic acid，（+）-syringaresinol-

-D-glucopyranoside and syringaresinol. The contents of the above five components were 0.047 2-0.401 6， 0.836 8-8.697 9， 1.245 3-10.950 0， 0.139 0-0.437 8 and 0.016 4-0.635 3 mg/g， respectively.

CONCLUSIONS

The established method is stable and accurate， which can be used for the quality control of the roots and rhizoma of

T. angulata

. It is preliminarily proposed that the moisture in the roots and rhizoma of

T. angulata

is not more than 11.0%， the total ash is not more than 4.0%， the ethanol-soluble extract is not less than 5.0%， the contents of coniferin， syringin， chlorogenic acid，（+）-syringaresinol-

-D-glucopyranoside and syringaresinol are not less than 0.04， 0.83， 1.24， 0.13， 0.01 mg/g， respectively.

关键词

水冬瓜根水冬瓜茎性状鉴别显微鉴别指纹图谱含量测定质量控制

Keywords

rhizoma of Toricellia angulataproperty identificationmicroscopic identificationfingerprintcontent determinationquality control

references

贵州省药品监督管理局. 贵州省中药材、民族药材质量标准：2003年版[M].贵阳：贵州科技出版社，2003：107.

Guizhou Medical Products Administration. Quality standard of Chinese medicinal materials and ethnic medicinal materials in Guizhou Province：2003 edition[M]. Guiyang：Guizhou Science and Technology Press，2003：107.

杨济秋，杨济中. 贵州民间方药集[M]. 贵阳：贵州科技出版社，1958：471.

YANG J Q，YANG J Z. Guizhou folk prescription drug collection[M]. Guiyang：Guizhou Science and Technology Press，1958：471.

王燕燕，涂念，张勇慧，等.大接骨丹化学成分研究：Ⅱ[J].中草药，2009，40（10）：1551-1553.

WANG Y Y，TU N，ZHANG Y H，et al. Studies on chemical constituents of Dajiegudan：Ⅱ[J]. Chin Tradit Herb Drugs，2009，40（10）：1551-1553.

韩忠耀，胡恩明，张林甦，等.苗药水冬瓜叶化学成分的HPLC-HESI-HRMS分析[J].中国现代应用药学，2022，39（13）：1721-1730.

HAN Z Y，HU E M，ZHANG L S，et al. Analysis on chemical components for the leaves of the Miao medicine Toricellia angulata Oliv. var. intermedia（Harms） Hu by HPLC-HESI-HRMS[J]. Chin J Mod Appl Pharm，2022，39（13）：1721-1730.

涂念.大接骨丹免疫调节活性成分研究[D]. 武汉：华中科技大学，2009.

TU N. Study on the active constituents of immunoregulation of Torricellia angulata[D]. Wuhan：Huazhong University of Science and Technology，2009.

韩忠耀，黄春丽，陆廷祥，等. 水冬瓜叶乙醇提取物不同萃取部位抗炎镇痛活性研究[J]. 山东化工，2022，51（14）：11-13.

HAN Z Y，HUANG C L，LU T X，et al. Research on anti-inflammatory and analgesic activity of different solvent extractions of ethanol extracts from the leaves of Toricellia angulata Oliv.var. intermedia（Harms） Hu[J]. Shandong Chem Ind，2022，51（14）：11-13.

韩忠耀，陆廷祥，张秋瑾，等. 苗药水冬瓜根皮药材质量标准提升研究[J]. 西部中医药，2019，32（3）：41-44.

HAN Z Y，LU T X，ZHANG Q J，et al. Improvement of quality standard for Miao medicine Toricellia angulata Oliv.[J]. West J Tradit Chin Med，2019，32（3）：41-44.

韩忠耀，陈菊，张林甦，等. 水冬瓜叶药材的质量标准研究[J]. 天津药学，2022，34（6）：5-10.

HAN Z Y，CHEN J，ZHANG L S，et al. Quality standard for the leaves of Toricellia angulata Oliv.var.intermedia（Harms） Hu[J]. Tianjin Pharm，2022，34（6）：5-10.

韩忠耀，曹芳，江品良，等. 基于高效液相色谱指纹图谱和化学模式识别法评价不同采收期水冬瓜树皮药材质量[J]. 理化检验-化学分册，2021，57（3）：204-209.

HAN Z Y，CAO F，JIANG P L，et al. Quality evaluation for the bark of Toricellia angulata Oliv. var. intermedia（Harms） Hu in different collecting seasons based on HPLC fingerprint and chemical pattern recognition[J]. Phys Test Chem Anal Part B Chem Anal，2021，57（3）：204-209.

陈华栋，张锦文，张勇慧. 大接骨丹干燥树皮抗炎镇痛活性有效部位筛选[J]. 海峡药学，2012，24（2）：29-30.

CHEN H D，ZHANG J W，ZHANG Y H. Research on the active extracts of Torricellia angulata Oliv.of anti-inflammatory and analgesic effects[J]. Strait Pharm J，2012，24（2）：29-30.

国家药典委员会.中华人民共和国药典：四部[M].2020年版.北京：中国医药科技出版社，2020：114，232，234.

Chinese Pharmacopoeia Committee. Chinese pharmacopoeia：part Ⅳ[M].2020 edition.Beijing：China Medical Science Press，2020：114，232，234.

黄建猷，胡筱希，谭晓，等. 瑶药消瘤藤的质量标准研究[J]. 中国药房，2021，32（21）：2624-2630.

HUANG J Y，HU X X，TAN X，et al. Study on quality standard of Yao medicine Pileostegia tomentella[J]. China Pharm，2021，32（21）：2624-2630.

陈启鑫，邵新田. 一种松柏苷的应用：CN112451536A[P]. 2021-03-09.

CHEN Q X，SHAO X T. Application of coniferin：CN112451536A[P]. 2021-03-09.

王菲，袁冲，杨艳芳，等. 紫丁香苷的植物资源、体内代谢途径及药理作用研究进展[J]. 中国药师，2021，24（11）：2073-2076.

WANG F，YUAN C，YANG Y F，et al. Overview of plant resources，metabolic pathways and pharmacological effects of syringin[J]. China Pharm，2021，24（11）：2073-2076.

BISHT A，DICKENS M，RUTHERFURD-MARKWICK K，et al. Chlorogenic acid potentiates the anti-inflammatory activity of curcumin in LPS-stimulated THP-1 cells[J]. Nutrients，2020，12（9）：2706.

塔娜. 宽筋藤的化学成分与药理作用研究[D]. 武汉：中南民族大学，2018.

TA N. Study on chemical constituents and pharmacological action of Tinpspora sinensis（Lour.）Merr.[D]. Wuhan：South-central University for Nationalities，2018.

潘明明，冯燕，李青梅.丁香脂素在Ⅱ型胶原诱导关节炎大鼠中的作用及相关机制[J]. 临床内科杂志，2022，39（10）：696-700.

PAN M M，FENG Y，LI Q M. Effect of syringaresinol on rats of type Ⅱ collagen-induced arthritis and related mechanism[J]. J Clin Intern Med，2022，39（10）：696-700.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Identification of components， fingerprint and content determination of the effective parts of Dracocephalum moldavica

Study on quality control method of Qiguiling mixture

Establishment of HPLC fingerprint and content determination of multiple index components in Xiao’er resuqing oral liquid

Extraction， isolation， identification and content determination of four chemical components in Yao medicine Ventilago leiocarpa

Establishment of HPLC fingerprint and content determination of 8 components in Jianpi huayu decoction

Related Author

HE Chenghui

LI Honghong

LYU Jiamin

ZOU Guoan

Hajiakber·Asia

YU Xiaotao

WANG Bo

WANG Jia

Related Institution

University of Chinese Academy of Sciences

Xinjiang Medicine Research Institute

State Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization， Xinjiang Technical Institute of Physics and Chemistry， Chinese Academy of Sciences

Dept. of Pharmacy， Luohe Central Hospital/Henan Engineering Research Center of TCM Preparation Modernization Technology R&D and Clinical Application

College of Pharmacy， Shandong University of Traditional Chinese Medicine

Address：8/F,129 Daping Main Street,Yuzhong District,Chongqing 400042,P.R.China
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Study on quality control method of the roots and rhizoma of Toricellia angulata

DOI：10.6039/j.issn.1001-0408.2024.01.04

摘要

Abstract

关键词

Keywords

references