Study on characteristic chromatogram of <italic style="font-style: italic">Chaenomeles sinensis </italic>and content determination of 3 flavones

ZHU Tianmi; CHEN Shuhe; YAN Jingsong; WANG Xingui; DUAN Yuqing; YANG Xiaoyi

doi:10.6039/j.issn.1001-0408.2024.02.05

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Study on characteristic chromatogram of Chaenomeles sinensis and content determination of 3 flavones

更新时间：2024-01-24

- Study on characteristic chromatogram of Chaenomeles sinensis and content determination of 3 flavones
- ZHONGGUO YAOFANG Vol. 35, Issue 2, Pages: 150-154(2024)
- 作者机构：
  
  1.湖北省中医院/湖北中医药大学附属医院/湖北省中医药研究院药事部，武汉　430061
  2.湖北中医药大学药学院，武汉　430065
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.02.05
  CLC： R917
- Published：30 January 2024，
  
  Received：06 June 2023，
  
  Revised：26 December 2023，
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朱田密,陈树和,严劲松等.光皮木瓜特征图谱及3种黄酮类成分含量测定研究 ^Δ[J].中国药房,2024,35(02):150-154.

ZHU Tianmi,CHEN Shuhe,YAN Jingsong,et al.Study on characteristic chromatogram of Chaenomeles sinensis and content determination of 3 flavones[J].ZHONGGUO YAOFANG,2024,35(02):150-154.
朱田密,陈树和,严劲松等.光皮木瓜特征图谱及3种黄酮类成分含量测定研究 ^Δ[J].中国药房,2024,35(02):150-154. DOI： 10.6039/j.issn.1001-0408.2024.02.05.

ZHU Tianmi,CHEN Shuhe,YAN Jingsong,et al.Study on characteristic chromatogram of Chaenomeles sinensis and content determination of 3 flavones[J].ZHONGGUO YAOFANG,2024,35(02):150-154. DOI： 10.6039/j.issn.1001-0408.2024.02.05.

摘要

目的

建立光皮木瓜的特征图谱，同时测定芦丁、金丝桃苷、槲皮苷3种黄酮类成分的含量，鉴别光皮木瓜和皱皮木瓜。

方法

采用高效液相色谱（HPLC）法。以Agilent 5 TC-C

为色谱柱，以乙腈-0.2%磷酸溶液为流动相进行梯度洗脱，流速为1.0 mL/min，柱温为30 ℃，特征图谱检测波长为330 nm，含量测定检测波长为350 nm。采用《中药色谱指纹图谱相似度评价系统（2012版）》建立光皮木瓜的特征图谱并评价相似度，使用SPSS 23.0软件对15批光皮木瓜（S1～S15）进行聚类分析；测定15批光皮木瓜和7批皱皮木瓜（S16～S22）中3种黄酮类成分的含量，比较二者特征图谱。

结果

15批光皮木瓜样品的特征图谱相似度为0.783～0.969；共确定11个特征峰；指认了4种成分，分别为绿原酸、芦丁、金丝桃苷、槲皮苷。15批光皮木瓜样品聚为2类，S5～S8为一类，其余为一类。光皮木瓜与皱皮木瓜的特征图谱有明显区别。15批光皮木瓜样品中芦丁、金丝桃苷、槲皮苷含量分别为48.99～294.45、3.49～102.55、31.98～149.49 μg/g，皱皮木瓜中芦丁含量低于光皮木瓜，且皱皮木瓜中均未检出金丝桃苷（S20样品除外）和槲皮苷。

结论

所建立的光皮木瓜的特征图谱和3种黄酮类成分的含量测定方法可用于其质量控制及其与皱皮木瓜的鉴别。

Abstract

OBJECTIVE

To establish the characteristic chromatogram of

Chaenomeles sinensis

， determine the contents of rutin， hyperin and quercitrin， and to identify

C. sinensis

and

C. speciosa

METHODS

HPLC method was performed on Agilent 5 TC-C

column， with acetonitrile-0.2% formic acid solution as the mobile phase for gradient elution， at the flow rate of 1.0 mL/min. The column temperature was 30 ℃. The detection wavelength was 330 nm in characteristic chromatogram and 350 nm in content determination. The characteristic chromatogram of

C. sinensis

was established and similarity was evaluated by the

Similarity Evaluation System for Chromatographic Fingerprint of TCM

（2012 edition）. Hierarchical cluster analysis of 15 batches of

C. sinensis

（S1-S15） was performed by using SPSS 23.0 software. The contents of 3 flavones in 15 batches of

C. sinensis

and

7 batches of

C. speciosa

（S16-S22） were determined， while their characteristic chromatograms were compared.

RESULTS

The similarities of the characteristic chromatogram for 15 batches of

C. sinensis

ranged from 0.783 to 0.969， and 11 characteristic peaks were confirmed. Four constituents were identified as chlorogenic acid， rutin， hyperin and quercitrin. The medicinal materials in 15 batches of

C. sinensis

could be divided into 2 categories： S5-S8 were one category， and the others belonged to one category. The characteristic chromatogram of

C. sinensis

was obviously different from

C. speciosa

. The contents of rutin， hyperin and quercitrin in 15 batches of

C. sinensis

were 48.99-294.45， 3.49-102.55， 31.98-149.49 μg/g， respectively. The content of rutin in

C. speciosa

was lower than that in

C. sinensis.

None of hyperin （except for S20） and quercitrin were detected in

C. speciosa

CONCLUSIONS

The characteristic chromatogram and the method for content determination of 3 flavones in

C. sinensis

are established successfully and can be used for the quality control of

C. sinensis

and its identification from

C. speciosa

关键词

光皮木瓜黄酮特征图谱高效液相色谱法

Keywords

flavonescharacteristic chromatogramHPLC

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Study on characteristic chromatogram of Chaenomeles sinensis and content determination of 3 flavones

DOI：10.6039/j.issn.1001-0408.2024.02.05

摘要

Abstract

关键词

Keywords

references