Study on metabolites derived from Zhideke granules in rats<italic style="font-style: italic"> in vivo</italic>

LIANG Jie; ZHENG Piaoxue; CHEN Huihua; HUANG Chunyan; LIANG Yanli; LU Chunlian; XIE Jingjing; MA Yuming; PENG Jiawen; ZHAO Lichun; CHEN Rilan

doi:10.6039/j.issn.1001-0408.2024.02.09

您当前的位置：

首页 >

文章列表页 >

Study on metabolites derived from Zhideke granules in rats in vivo

更新时间：2024-01-24

- Study on metabolites derived from Zhideke granules in rats in vivo
- ZHONGGUO YAOFANG Vol. 35, Issue 2, Pages: 172-178(2024)
- 作者机构：
  
  1.广西中医药大学药学院，南宁　530200
  2.广西高校中药提取纯化与质量分析重点实验室，南宁　530200
  3.中药固体制剂制造技术国家工程研究中心华南分中心，南宁　530200
  4.广西壮瑶药工程技术研究中心，南宁　530200
  5.钦州市检验检测中心，广西钦州　535000
  6.广西中医药大学附属瑞康医院药剂科，南宁　530011
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.02.09
  CLC： R969.1
- Published：30 January 2024，
  
  Received：26 June 2023，
  
  Revised：12 October 2023，
扫描看全文
梁洁,郑飘雪,陈辉华等.止得咳颗粒在大鼠体内的代谢产物研究 ^Δ[J].中国药房,2024,35(02):172-178.

LIANG Jie,ZHENG Piaoxue,CHEN Huihua,et al.Study on metabolites derived from Zhideke granules in rats in vivo[J].ZHONGGUO YAOFANG,2024,35(02):172-178.
梁洁,郑飘雪,陈辉华等.止得咳颗粒在大鼠体内的代谢产物研究 ^Δ[J].中国药房,2024,35(02):172-178. DOI： 10.6039/j.issn.1001-0408.2024.02.09.

LIANG Jie,ZHENG Piaoxue,CHEN Huihua,et al.Study on metabolites derived from Zhideke granules in rats in vivo[J].ZHONGGUO YAOFANG,2024,35(02):172-178. DOI： 10.6039/j.issn.1001-0408.2024.02.09.

摘要

目的

分析止得咳颗粒在大鼠体内的代谢产物并推测其代谢途径。

方法

将雄性SD大鼠随机分为空白组、给药组（止得咳颗粒，9.45 g/kg），每组灌胃超纯水或相应药液，每天2次，每次灌胃间隔6～8 h，连续3 d。收集各组大鼠血清、粪便、尿液样品，利用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱（UPLC-Q-Exactive-MS）技术鉴定大鼠灌胃止得咳颗粒后上述生物样品中的代谢产物，并推测其代谢途径。

结果

大鼠灌胃止得咳颗粒后，在其血清、尿液、粪便样品中共鉴定出16个原型成分（如野鸢尾黄素、黄芩素、绿原酸等）和11个代谢产物（如山柰酚或木犀草素水合产物、绿原酸甲基化产物、黄芩苷羟基化产物）。其中，血清样品中鉴定出8个原型成分和4个代谢产物；尿液样品中鉴定出10个原型成分和7个代谢产物；粪便样品中鉴定出8个原型成分和5个代谢产物。

结论

止得咳颗粒在大鼠体内的代谢成分主要包括黄芩苷、野鸢尾黄素、绿原酸，主要涉及甲基化、羟基化、葡萄糖醛酸化等代谢途径。

Abstract

OBJECTIVE

To analyze the metabolites of Zhideke granules and speculate its metabolic pathway in rats

in vivo

METHODS

Male SD rats were randomly divided into blank group and administration group （Zhideke granules， 9.45 g/kg）； they were given ultrapure water or relevant medicine， twice a day， every 6-8 h， for 3 consecutive days. Serum， urine and feces samples of rats were collected， and their metabolites were identified by UPLC-Q-Exactive-MS technique after intragastric administration of Zhideke granules； their metabolic pathways were speculated.

RESULTS

After intragastric administration of Zhideke granules， 16 prototype components （i.g. irisflorentin， baicalin， chlorogenic acid） and 11 metabolites （i.g. hydration products of kaempferol or luteolin， methylation products of chlorogenic acid， and hydroxylation products of baicalin） were identified in serum， urine and feces of rats. Among them， 8 prototype components and 4 metabolites were identified in serum samples； 10 prototype components and 7 metabolites were identified in urine samples； 8 prototype components and 5 metabolites were identified in the fecal samples.

CONCLUSIONS

The metabolites of Zhideke granules in rats mainly include baicalin， irisflorentin， chlorogenic acid， and the main metabolic pathways included methylation， hydroxylation， glucuronidation.

关键词

止得咳颗粒超高效液相色谱-四极杆-静电场轨道阱高分辨质谱技术代谢产物代谢途径大鼠

Keywords

UPLC-Q-Exactive-MSmetabolitesmetabolic pathwayrat

references

辛静，李丽丽，凌爱思. 止得咳冲剂治疗支气管哮喘急性发作期的临床观察[J]. 广西中医药大学学报，2013，16（1）：35-36.

XIN J，LI L L，LING A S. Clinical observation on Zhideke granule in the treatment of acute attack of bronchial asthma[J]. J Guangxi Univ Chin Med，2013，16（1）：35-36.

周昱杉，梁洁，黄光强，等. 基于化学模式识别方法分析止得咳颗粒的抗炎谱效关系[J]. 中国实验方剂学杂志，2020，26（22）：156-163.

ZHOU Y S，LIANG J，HUANG G Q，et al. Analysis on spectrum-effect relationship of anti-inflammatory effect of Zhideke granules based on chemical pattern recognition[J]. Chin J Exp Tradit Med Formulae，2020，26（22）：156-163.

梁洁，陈辉华，徐晖，等. 止得咳颗粒提取工艺的优化[J]. 中国药房，2021，32（7）：832-838.

LIANG J，CHEN H H，XU H，et al. Optimization of the extraction technology of Zhideke granules[J]. China Pharm，2021，32（7）：832-838.

梁洁，陈辉华，信晨曦，等. 止得咳颗粒止咳作用研究[J]. 广西中医药，2022，45（2）：64-67.

LIANG J，CHEN H H，XIN C X，et al. Research on the antitussive effect of Zhideke granules[J]. Guangxi J Tradit Chin Med，2022，45（2）：64-67.

黄光强，梁洁，孙正伊，等. 基于超高效液相色谱-四极杆/静电场轨道阱高分辨质谱技术的止得咳颗粒化学成分研究[J]. 中南药学，2021，19（5）：856-864.

HUANG G Q，LIANG J，SUN Z Y，et al. Chemical constituents in Zhideke granules by UPLC-Q-Orbitrap HRMS[J]. Cent South Pharm，2021，19（5）：856-864.

刘月程，田振华. 超高效液相色谱-四极杆静电场轨道阱质谱法快速鉴定柴胡化学成分[J]. 化学分析计量，2022，31（8）：5-12.

LIU Y C，TIAN Z H. Rapid identification of chemical constituents in Bupleuri Radix by UPLC-quadrupole/exactive orbitrap mass spectrometry[J]. Chem Anal Meterage，2022，31（8）：5-12.

孙志，周霖，禹明洋，等. 基于UHPLC-Q-Orbitrap HRMS技术的6-姜酚在大鼠体内代谢产物的鉴定及代谢途径的分析[J]. 中草药，2021，52（24）：7420-7427.

SUN Z，ZHOU L，YU M Y，et al. Metabolites and metabolic pathway analysis of 6-gingerol in rats based on UHPLC-Q-Orbitrap HRMS[J]. Chin Tradit Herb Drugs，2021，52（24）：7420-7427.

魏文峰，刘烨，霍金海，等. UPLC-Q-TOF-MS法分析大鼠口服芩百清肺浓缩丸后肺组织中的化学成分[J]. 中草药，2018，49（5）：1115-1122.

WEI W F，LIU Y，HUO J H，et al. Analysis on chemical components in rat lung tissue after oral administration of Qinbai qingfei concentrated pellets based on UPLC-Q-TOF-MS[J]. Chin Tradit Herb Drugs，2018，49（5）：1115-1122.

孙亮. 痰热清注射液体内代谢与相互作用研究[D]. 上海：第二军医大学，2013.

SUN L. Studies on metabolic characteristics of tanreqing injection and its interaction with sirolimus in rats[D]. Shanghai：Second Military Medical University，2013.

高文雅，李涛，周严严，等. 黄芩水煎液中化学成分的定性及定量研究[J]. 中草药，2022，53（23）：7339-7352.

GAO W Y，LI T，ZHOU Y Y，et al. Qualitative and quantitative study on chemical constituents in Scutellariae Radix decoction[J]. Chin Tradit Herb Drugs，2022，53（23）：7339-7352.

李森林，刘春明，李赛男，等. 射干中乳酸脱氢酶抑制剂的筛选分离与质谱分析[J]. 天然产物研究与开发，2017，29（4）：595-600.

LI S L，LIU C M，LI S N，et al. Screening and identification of lactate dehydrogenase inhibitors from Belamcanda chinensis extract using HPLC-ESI-MS[J]. Nat Prod Res Dev，2017，29（4）：595-600.

高淑红，苏珍枝，杨琳娇，等. 基于液质联用和核磁共振的龙葵茎化学成分研究[J]. 中草药，2021，52（5）：1263-1273.

GAO S H，SU Z Z，YANG L J，et al. Chemical com-ponents from stems of Solanum nigrum by LC-MS and NMR[J]. Chin Tradit Herb Drugs，2021，52（5）：1263-1273.

邱佳佳，刘军民，詹若挺，等. 基于UPLC-Q-TOF-MS和UPLC-DAD的不同品种溪黄草主要化学成分分析[J]. 中国中药杂志，2022，47（13）：3539-3547.

QIU J J，LIU J M，ZHAN R T，et al. Main components in different varieties of Xihuangcao by UPLC-Q-TOF-MS and UPLC-DAD[J]. China J Chin Mater Med，2022，47（13）：3539-3547.

曲佳琳. 粉葛花化学成分及其活性成分鸢尾苷大鼠体内代谢的研究[D]. 沈阳：沈阳药科大学，2014.

QU J L. Studies on chemical constituents of flower of Pue-rariae thomsonii Benth. and metabolism of tectoridin in rats[D]. Shenyang：Shenyang Pharmaceutical University，2014.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Yiru tiaojing granules on the pharmacokinetics of olanzapine in rats

Improvement effects of Shangke xiaoyan hydrogel plaster on osteoarthritis model rats

Study on protective effects of twin drugs of tetramethylpyrazine-scutellarein on cerebral ischemia-reperfusion injury model rats and its mechanism

Intervention effect and mechanism of Zhongfeng yure decoction on ischemic stroke model rats

Improvement effect and mechanism of different extracts from Tylophora yunnanensis on non-alcoholic steatohepatitis

Related Author

ZHENG Huihui

ZHOU Longyue

ZHAO Boxin

WANG Chunxia

LIU Wei

ZOU Kaiping

WANG Qiong

WANG Rongzhou

Related Institution

Dept. of Pharmacy， Nanfang Hospital of Southern Medical University

Key Laboratory of Pharmaceutical Biofunctional Materials， School of Science， China Pharmaceutical University

Dept. of Preparation， Jiangsu Provincial Hospital of Traditional Chinese Medicine/the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine

School of Pharmacy， Guizhou Medical University

Dept. of Pharmacy， Shouguang Municipal People’s Hospital of Shandong Province， Shandong Shouguang

Address：8/F,129 Daping Main Street,Yuzhong District,Chongqing 400042,P.R.China
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Study on metabolites derived from Zhideke granules in rats in vivo

DOI：10.6039/j.issn.1001-0408.2024.02.09

摘要

Abstract

关键词

Keywords

references