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Study on the antifungal activity and mechanism of Huangqin decoction against
Trichophyton mentagrophytes - ZHONGGUO YAOFANG Vol. 35, Issue 3, Pages: 311-315(2024)
- 作者机构：
1.江西省人民医院（南昌医学院第一附属医院）药学部，南昌;330006
2.南昌大学药学院，南昌 330006
3.江西省人民医院（南昌医学院第一附属医院）临床医学研究所，南昌 330006
4.江西中医药大学现代中药制剂教育部重点实验室，南昌 330004
- 作者简介：
- 基金信息：
DOI：10.6039/j.issn.1001-0408.2024.03.08
CLC： R275.9;R965Published：15 February 2024，
Received：03 August 2023，
Revised：22 December 2023，
扫 描 看 全 文
沈成英,罗忠,章佩等.黄芩汤的抗须癣毛癣菌活性及作用机制研究 Δ[J].中国药房,2024,35(03):311-315.
SHEN Chengying,LUO Zhong,ZHANG Pei,et al.Study on the antifungal activity and mechanism of Huangqin decoction against Trichophyton mentagrophytes[J].ZHONGGUO YAOFANG,2024,35(03):311-315.
沈成英,罗忠,章佩等.黄芩汤的抗须癣毛癣菌活性及作用机制研究 Δ[J].中国药房,2024,35(03):311-315. DOI： 10.6039/j.issn.1001-0408.2024.03.08.
SHEN Chengying,LUO Zhong,ZHANG Pei,et al.Study on the antifungal activity and mechanism of Huangqin decoction against Trichophyton mentagrophytes[J].ZHONGGUO YAOFANG,2024,35(03):311-315. DOI： 10.6039/j.issn.1001-0408.2024.03.08.
摘要
研究黄芩汤（HQD）的抗须癣毛癣菌活性及作用机制。
方法
2
通过测定最小抑菌浓度（MIC）、最小杀菌浓度（MFC）、菌丝长度、孢子萌发率、生物量和观察菌丝超微结构评价HQD的抗须癣毛癣菌活性；通过山梨醇保护实验检测HQD对须癣毛癣菌细胞壁的影响；通过测定麦角固醇含量和角鲨烯环氧酶（SE）、羊毛甾醇14
α
-去甲基化酶（CYP51）的活性考察HQD对须癣毛癣菌细胞膜的影响；通过测定线粒体中苹果酸脱氢酶（MDH）、琥珀酸脱氢酶（SDH）及ATP酶（包括钠钾ATP酶、钙镁ATP酶和总ATP酶）的活性考察HQD对须癣毛癣菌线粒体的影响。
结果
2
HQD对须癣毛癣菌具有显著的抑菌活性，MIC、MFC值分别为3.13、25 mg/mL。经HQD干预后，须癣毛癣菌菌丝长度均显著缩短（
P
＜0.05）；孢子萌发率、生物量、细胞膜中麦角固醇含量和SE、CYP51活性以及线粒体中MDH、SDH、各种ATP酶的活性均显著降低（
P
＜0.05）；细胞结构受到了一定程度的破坏，但细胞壁的完整性没有受影响。
结论
2
HQD具有显著的抗须癣毛癣菌活性，其作用机制与降低细胞膜中麦角固醇含量和SE、CYP51活性以及线粒体相关酶活性有关。
Abstract
OBJECTIVE
2
To study the antifungal activity of Huangqin decoction （HQD） against
Trichophyton mentagrophytes
and explore its mechanism.
METHODS
2
Minimal inhibitory concentration （MIC）， minimal fungicidal concentration （MFC）， mycelial length， spore germination rate， biomass and mycelium ultrastructure observation were performed to evaluate the antifungal activity of HQD against
T. mentagrophytes.
The effects of HQD on the cell wall of
T. mentagrophytes
were detected through sorbitol protection experiment. By measuring the content of ergosterol and the activities of squalene epoxide （SE） and lanosterol 14
α
-demethylase （CYP51）， the activity of HQD on the cell membrane of
T. mentagrophytes
was investigated. The effects of HQD on
T. mentagrophytes
mitochondria were investigated by determining the activities of malate dehydrogenase （MDH）， succinate dehydrogenase （SDH）， and ATPases （including sodium potassium ATPase， calcium magnesium ATPase， and total ATPase）.
RESULTS
2
HQD exhibited significant antifungal activity against
T. mentagrophytes
with MIC of 3.13 mg/mL and MFC of 25 mg/mL. After intervention with HQD， the mycelial length of
T. mentagrophytes
was significantly shortened （
P
＜0.05）； spore germination rate， biomass， the content of ergosterol in the cell membrane， the activities of SE and CYP51 in the cell membrane and MDH， SDH and ATPase in mitochondria were all decreased significantly （
P
＜0.05）； cell structure had been damaged to a certain extent， but the integrity of the cell wall had not been affected.
CONCLUSIONS
2
HQD shows significant antifungal activity against
T. mentagrophytes
， the mechanism of which may be associated with reducing the content of ergosterol in the cell membrane and the activities of SE， CYP51， and mitochondria-related enzymes.
关键词
黄芩汤须癣毛癣菌抗真菌活性作用机制细胞膜线粒体
Keywords
Trichophyton mentagrophytesantifungal activitymechanism of actioncell membranemitochondria
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