Effects of quercetin on mitochondrial energy metabolism function after myocardial ischemia

WANG Panpan; YANG Zan; LIU Donglan; ZHOU Yi

doi:10.6039/j.issn.1001-0408.2024.04.04

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Effects of quercetin on mitochondrial energy metabolism function after myocardial ischemia

更新时间：2024-02-23

- Effects of quercetin on mitochondrial energy metabolism function after myocardial ischemia
- ZHONGGUO YAOFANG Vol. 35, Issue 4, Pages: 401-406(2024)
- 作者机构：
  
  青海大学生态环境工程学院，西宁　810016
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.04.04
  CLC： R965
- Published：29 February 2024，
  
  Received：24 July 2023，
  
  Revised：24 January 2024，
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王盼盼,杨赞,刘冬兰等.槲皮素对心肌缺血后线粒体能量代谢功能的影响 ^Δ[J].中国药房,2024,35(04):401-406.

WANG Panpan,YANG Zan,LIU Donglan,et al.Effects of quercetin on mitochondrial energy metabolism function after myocardial ischemia[J].ZHONGGUO YAOFANG,2024,35(04):401-406.
王盼盼,杨赞,刘冬兰等.槲皮素对心肌缺血后线粒体能量代谢功能的影响 ^Δ[J].中国药房,2024,35(04):401-406. DOI： 10.6039/j.issn.1001-0408.2024.04.04.

WANG Panpan,YANG Zan,LIU Donglan,et al.Effects of quercetin on mitochondrial energy metabolism function after myocardial ischemia[J].ZHONGGUO YAOFANG,2024,35(04):401-406. DOI： 10.6039/j.issn.1001-0408.2024.04.04.

摘要

目的

探究槲皮素对心肌缺血后线粒体能量代谢功能的影响。

方法

将H9c2心肌细胞分为空白组，模型组，槲皮素高、中、低剂量组（40、20、10 μmol/L）和阳性对照组（环孢菌素A 1 μmol/L），细胞缺氧处理后，检测活性氧（ROS）、线粒体膜电位（MMP）、线粒体膜通透性转换孔（MPTP）开放程度、腺苷三磷酸（ATP）、丙二醛（MDA）、乳酸脱氢酶（LDH）和肌酸激酶（CK）的变化。将大鼠随机分为假手术组，模型组，槲皮素高、中、低剂量组（100、50、25 mg/kg）和阳性对照组（曲美他嗪6.3 mg/kg），每组8只，灌胃相应药物，每天1次，连续7 d；大鼠末次给药后，通过冠状动脉左前降支结扎建立心肌缺血模型，检测血清中LDH、MDA、肌酸激酶同工酶MB（CK-MB）、超氧化物歧化酶（SOD）、线粒体呼吸链复合物Ⅰ（complex Ⅰ）、complex Ⅳ和ATP的含量。

结果

与模型组比较，槲皮素高、中、低剂量组和阳性对照组细胞中ROS荧光强度、MPTP开放程度和CK、LDH、MDA含量均显著降低，MMP和ATP含量均显著升高（

＜0.01）；槲皮素高、中、低剂量组和阳性对照组大鼠血清中CK-MB、LDH和MDA含量均显著降低，SOD、complex Ⅰ、complex Ⅳ和ATP（阳性对照组除外）含量均显著升高（

＜0.05或

＜0.01）。

结论

槲皮素能有效减轻心肌缺氧损伤，促进内源性能量的生成，改善心肌缺血后线粒体功能。

Abstract

OBJECTIVE

To investigate the effects of quercetin on mitochondrial energy metabolism function after myocardial ischemia.

METHODS

H9c2 cells were divided into blank group， model group， quercetin high-dose， medium-dose and low-dose groups （40， 20， 10 μmol/L）， and positive control group （cyclosporine A， 1 μmol/L）. Reactive oxygen species （ROS）， mitochondrial membrane potential （MMP）， openness of mitochondrial permeability transition pore （MPTP）， adenosine triphosphate （ATP）， malondialdehyde （MDA）， lactate dehydrogenase （LDH） and creatine kinase （CK） were observed after cell hypoxia treatment. Rats were randomly assigned into sham operation group， model group， quercetin high-dose， medium-dose and low-dose groups （100， 50， 25 mg/kg）， and positive control group （trimetazidine， 6.3 mg/kg）， with 8 rats in each group. They were given relevant medicine intragastrically， once a day， for 7 consecutive days. After the last medication， myocardial ischemia model was induced by the ligation of the left anterior descending branch of the coronary artery. The contents of LDH， MDA， creatine kinase isoenzyme-MB （CK-MB）， superoxide dismutase （SOD）， complex Ⅰ， complex Ⅳ and ATP in serum were all determined.

RESULTS

Compared with the model group， ROS fluorescence intensity， openness of MPTP， the contents of CK， LDH and MDA were significantly decreased in quercetin low-dose， medium-dose and high-dose groups， and positive control group， while the contents of MMP and ATP were all increased significantly （

＜0.01）； the contents of CK-MB， LDH and MDA in serum were all decreased significantly in quercetin low-dose， medium-dose and high-dose groups， and positive control group， while the contents of SOD， complex Ⅰ， complex Ⅳ and ATP （except for positive control group） were increased significantly （

＜0.05 or

＜0.01）.

CONCLUSIONS

Quercetin can effectively reduce myocardial hypoxic injury， promote endogenous energy production and improve mitochondrial function after myocardial ischemia.

关键词

槲皮素心肌缺血缺血性心脏病线粒体能量代谢

Keywords

myocardial ischemiamyocardial ischemiamitochondriaenergy metabolism

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