Effect of volatile oil of <italic style="font-style: italic">Ligusticum chuanxiong</italic> on transdermal properties and cytotoxicity of triptolide <italic style="font-style: italic">in vitro</italic>

JIANG Cheng; ZANG Zhenzhong; CHEN Lihua; ZHU Haiting; FU Shihua; ZHU Weifeng; WU Wenting; SHI Wei; GUAN Yongmei

doi:10.6039/j.issn.1001-0408.2024.04.06

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Effect of volatile oil of Ligusticum chuanxiong on transdermal properties and cytotoxicity of triptolide in vitro

更新时间：2024-02-23

- Effect of volatile oil of Ligusticum chuanxiong on transdermal properties and cytotoxicity of triptolide in vitro
- ZHONGGUO YAOFANG Vol. 35, Issue 4, Pages: 413-418(2024)
- 作者机构：
  
  1.江西中医药大学现代中药制剂教育部重点实验室，南昌　330004
  2.江西中医药大学药学院，南昌　330004
  3.福建中医药大学附属康复医院药学部，福州　350003
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.04.06
  CLC： R965
- Published：29 February 2024，
  
  Received：28 July 2023，
  
  Revised：11 December 2023，
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蒋成,臧振中,陈丽华等.川芎挥发油对雷公藤甲素体外透皮性能及细胞毒性的影响 ^Δ[J].中国药房,2024,35(04):413-418.

JIANG Cheng,ZANG Zhenzhong,CHEN Lihua,et al.Effect of volatile oil of Ligusticum chuanxiong on transdermal properties and cytotoxicity of triptolide in vitro[J].ZHONGGUO YAOFANG,2024,35(04):413-418.
蒋成,臧振中,陈丽华等.川芎挥发油对雷公藤甲素体外透皮性能及细胞毒性的影响 ^Δ[J].中国药房,2024,35(04):413-418. DOI： 10.6039/j.issn.1001-0408.2024.04.06.

JIANG Cheng,ZANG Zhenzhong,CHEN Lihua,et al.Effect of volatile oil of Ligusticum chuanxiong on transdermal properties and cytotoxicity of triptolide in vitro[J].ZHONGGUO YAOFANG,2024,35(04):413-418. DOI： 10.6039/j.issn.1001-0408.2024.04.06.

摘要

目的

探究川芎挥发油对雷公藤甲素体外透皮性能和细胞毒性的影响。

方法

采用气相色谱-质谱联用法对川芎挥发油的化学成分进行分析。分离KM小鼠下腹皮肤，分为雷公藤甲素、雷公藤甲素配伍川芎挥发油1∶10、1∶50、1∶100组（以下简称“配伍1∶10”“配伍1∶50”“配伍1∶100”组），将各组小鼠皮肤与相应乳膏各0.2 g充分接触24 h后，采用高效液相色谱法测定各组接收液中雷公藤甲素的累计透皮量（

），并计算透皮吸收速率（

）；再以人永生化角质形成细胞（HaCat）为模型，采用CCK-8法检测不同浓度川芎挥发油和雷公藤甲素配伍前后的细胞存活率。

结果

共鉴定出62个川芎挥发油化学成分，包括

-藁本内酯、洋川芎内酯、

-瑟林烯等。与雷公藤甲素组比较，配伍1∶10组和配伍1∶50组24 h内雷公藤甲素的

（

＜0.01）和

有所升高，而配伍1∶100组的

（

＜0.05）和

有所下降。与雷公藤甲素组比较，雷公藤甲素浓度在36、72、144 ng/mL时，配伍1∶10组和配伍1∶50组可以显著提高HaCat细胞的存活率（

＜0.05或

＜0.01），而配伍1∶100组细胞的存活率反而降低，但差异无统计学意义（

＞0.05）。

结论

雷公藤甲素与川芎挥发油在配伍比例为1∶10和1∶50时可以促进雷公藤甲素的透皮吸收，且可降低雷公藤甲素对HaCat细胞的毒性。

Abstract

OBJECTIVE

To explore the effect of volatile oil of

Ligusticum chuanxiong

on the transdermal properties and cytotoxicity of triptolide

in vitro

METHODS

The chemical constituents of the volatile oil of

L. chuanxiong

were analyzed by gas chromatography-mass spectrometry. The lower abdominal skin of KM mice was separated and divided into triptolide group， triptolide in compatibility with volatile oil of

L. chuanxiong

groups at 1∶10， 1∶50， 1∶100 （hereinafter referred to as “compatibility 1∶10”“compatibility 1∶50”“compatibility 1∶100” groups）. After the skin of mice in each group was fully exposed to 0.2 g of the corresponding cream for 24 h， the cumulative transdermal dose （

） of triptolide in the receiving solution of each group was determined by high-performance liquid chromatography， and the transdermal absorption rate （

） was calculated. Human immortalized keratinocytes （HaCat） were used as a model， the CCK-8 method was used to detect the cell survival rate of different concentrations of the volatile oil of

L. chuanxiong

and triptolide before and after compatibility.

RESULTS

A total of 62 chemical constituents of the volatile oil of

L. chuanxiong

were identified， including

-ligustilide， senkyunolide， and

-selinene. The

（

＜0.01） and

of triptolide increased within 24 h in the compatibility 1∶10 and 1∶50 groups， while the

（

＜0.05） and

decreased in the compatibility 1∶100 group as compared with the triptolide group. Compared with the triptolide group， the cell survival rate of HaCat was significantly increased in the compatibility 1∶10 and 1∶50 groups when the triptolide concentrations were 36， 72 and 144 ng/mL （

＜0.05 or

＜0.01）； while the cell survival rate of HaCat was decreased in the compatibility 1∶100 group， but the difference was not statistically significant （

＞0.05）.

CONCLUSIONS

When the compatibility ratio of triptolide and volatile oil of

L. chuanxiong

was 1∶10 or 1∶50， it can promote the transdermal absorption of triptolide and reduce the cytotoxicity of triptolide to HaCat.

关键词

川芎挥发油雷公藤甲素透皮吸收配伍增效减毒细胞毒性

Keywords

triptolidetransdermal absorptioncompatibilitysynergism and attenuationcytotoxicity

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⁰

Effect of volatile oil of Ligusticum chuanxiong on transdermal properties and cytotoxicity of triptolide in vitro

DOI：10.6039/j.issn.1001-0408.2024.04.06

摘要

Abstract

关键词

Keywords

references