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Anti-inflammatory activity and mechanism of
Clematis ranunculoides extract- ZHONGGUO YAOFANG Vol. 35, Issue 4, Pages: 453-458(2024)
- 作者机构：
1.云南省食品药品审核查验中心，昆明 650106
2.云南省药物研究所药理室， 昆明 650111
3.云南中医药大学动物实验中心，昆明 650500
- 作者简介：
- 基金信息：
DOI：10.6039/j.issn.1001-0408.2024.04.13
CLC： R965;R285Published：29 February 2024，
Received：22 August 2023，
Revised：10 January 2024，
扫 描 看 全 文
李海山,杨和金,郑永仁.绣球藤提取物的抗炎活性及机制 Δ[J].中国药房,2024,35(04):453-458.
LI Haishan,YANG Hejin,ZHENG Yongren.Anti-inflammatory activity and mechanism of Clematis ranunculoides extract[J].ZHONGGUO YAOFANG,2024,35(04):453-458.
李海山,杨和金,郑永仁.绣球藤提取物的抗炎活性及机制 Δ[J].中国药房,2024,35(04):453-458. DOI： 10.6039/j.issn.1001-0408.2024.04.13.
LI Haishan,YANG Hejin,ZHENG Yongren.Anti-inflammatory activity and mechanism of Clematis ranunculoides extract[J].ZHONGGUO YAOFANG,2024,35(04):453-458. DOI： 10.6039/j.issn.1001-0408.2024.04.13.
摘要
目的
2
探讨绣球藤提取物的抗炎活性及潜在机制。
方法
2
使用二甲苯致耳廓肿胀建立急性炎症小鼠模型，以阿司匹林（0.25 g/kg）为阳性对照，1.25、2.5、5 g/kg绣球藤提取物对小鼠耳肿胀度的影响。以植入棉球的方法建立慢性炎症大鼠模型，以阿司匹林（0.17 g/kg）为阳性对照，0.88、1.75、3.5 g/kg绣球藤提取物对大鼠肉芽净重的影响。以脂多糖诱导RAW264.7细胞建立炎症损伤细胞模型，考察12.5、25、50 μg/mL绣球藤提取物对细胞上清液中一氧化氮（NO）、前列腺素E
2
（PGE
2
）、肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）、单核细胞趋化蛋白1（MCP-1）含量，细胞中诱导型一氧化氮合酶（iNOS）、环氧合酶2（COX-2）、p65、磷酸化p65（p-p65）蛋白表达水平，以及p65蛋白核转移的影响。
结果
2
5 g/kg的绣球藤提取物可显著降低小鼠的耳肿胀度，1.75、3.5 g/kg的绣球藤提取物可显著降低大鼠的肉芽净重（
P
＜0.05）。12.5、25、50 μg/mL绣球藤提取物可显著降低细胞上清液中NO（12.5 μg/mL绣球藤提取物除外）、PGE
2
、TNF-α、IL-6、MCP-1含量，以及细胞中iNOS、COX-2蛋白的相对表达量和p-p65、p65蛋白的相对表达量比值（
P
＜0.05或
P
＜0.01）；25、50 μg/mL绣球藤提取物可阻断p65蛋白的核转移。
结论
2
绣球藤提取物具有良好的抗炎活性，其作用机制可能与抑制核因子κB信号通路激活、下调COX-2和iNOS蛋白的表达、减少炎症因子的释放有关。
Abstract
OBJECTIVE
2
To investigate the anti-inflammatory activity and potential mechanism of
Clematis ranunculoides
extract.
METHODS
2
The ear swelling was induced by xylene to establish an acute inflammation model of mice； using aspirin （0.25 g/kg） as a positive control， the effects of 1.25， 2.5， 5 g/kg
C. ranunculoides
extract on the degree of ear swelling were investigated. The chronic inflammation model of rats was also established by implanting cotton balls； using aspirin （0.17 g/kg） as a positive control， the effects of 0.88， 1.75， 3.5 g/kg
C. ranunculoides
extract on the net weight of granulomas were investigated. Furthermore， RAW264.7 cells were induced by lipopolysaccharide to establish an inflammatory injury model； the effects of 12.5， 25， 50 μg/mL
C. ranunculoides
extract on the contents of nitric oxide（NO）， prostaglandin E
2
（PGE
2
）， tumor necrosis factor-α（TNF-α）， interleukin-6（IL-6） and monocyte chemotactic protein-1（MCP-1） in the cell supernatant， the protein expressions of inducible nitric oxide synthase（iNOS）， cyclooxygenase-2（COX-2）， p65 and phosphorylated p65（p-p65） in cells as well as nuclear translocation of p65 protein were assessed.
RESULTS
2
C. ranunculoides
extract with 5 g/kg significantly relieved ear swelling in mice， and
C. ranunculoides
extract with 1.75， 3.5 g/kg significantly decreased the net weight of granulomas in rats （
P
＜0.05）.
C. ranunculoides
extract with 12.5， 25， 50 μg/mL significantly reduced the contents of NO （except for 12.5 μg/mL
C. ranunculoides
extract）， PGE
2
， TNF-α， IL-6 and MCP-1 in the cell supernatant， as well as the relative expressions of iNOS and COX-2 protein， and relative expression ratio of p-p65 and p65 protein （
P
＜0.05 or
P
＜0.01）；
C. ranunculoides
extract with 25， 50 μg/mL inhibited the translocation of p65 protein to the cell nucleus.
CONCLUSIONS
2
C. ranunculoides
extract exhibits significant anti-inflammatory activity， the mechanism of which may be attributed to the inhibition of the activation of nuclear factor-κB signaling pathway， down-regulation of COX-2 and iNOS protein expression， and the reduction of inflammatory cytokines release.
关键词
绣球藤提取物抗炎活性巨噬细胞核因子κB信号通路
Keywords
anti-inflammatory activitymacrophagesnuclear factor-κB signaling pathway
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