Establishment of a method for detecting propofol concentration in plasma and its application in patients with lymphedema

LI Jingfeng; SHI Zhengyuan; ZHANG Mengjie; GUAN Lei; JIANG Dechun

doi:10.6039/j.issn.1001-0408.2024.04.18

您当前的位置：

首页 >

文章列表页 >

Establishment of a method for detecting propofol concentration in plasma and its application in patients with lymphedema

更新时间：2024-02-23

- Establishment of a method for detecting propofol concentration in plasma and its application in patients with lymphedema
- ZHONGGUO YAOFANG Vol. 35, Issue 4, Pages: 476-480(2024)
- 作者机构：
  
  1.首都医科大学附属北京世纪坛医院药剂科，北京　100038
  2.首都医科大学附属北京世纪坛医院麻醉科，北京　100038
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.04.18
  CLC： R969.3;R917
- Published：29 February 2024，
  
  Received：24 August 2023，
  
  Revised：27 January 2024，
扫描看全文
李京峰,时正媛,张梦洁等.丙泊酚血药浓度检测方法建立及在淋巴水肿患者中的应用 ^Δ[J].中国药房,2024,35(04):476-480.

LI Jingfeng,SHI Zhengyuan,ZHANG Mengjie,et al.Establishment of a method for detecting propofol concentration in plasma and its application in patients with lymphedema[J].ZHONGGUO YAOFANG,2024,35(04):476-480.
李京峰,时正媛,张梦洁等.丙泊酚血药浓度检测方法建立及在淋巴水肿患者中的应用 ^Δ[J].中国药房,2024,35(04):476-480. DOI： 10.6039/j.issn.1001-0408.2024.04.18.

LI Jingfeng,SHI Zhengyuan,ZHANG Mengjie,et al.Establishment of a method for detecting propofol concentration in plasma and its application in patients with lymphedema[J].ZHONGGUO YAOFANG,2024,35(04):476-480. DOI： 10.6039/j.issn.1001-0408.2024.04.18.

摘要

目的

建立测定人血浆中丙泊酚浓度的方法并应用于淋巴水肿患者血药浓度检测。

方法

以麝香草酚为内标，血浆样品经蛋白沉淀后，采用超高效液相色谱-串联质谱（UPLC-MS/MS）法测定丙泊酚的浓度。以Kinetex C

为色谱柱，以乙腈（A）-水（B）为流动相进行梯度洗脱，流速为200 μL/min，进样量为5 μL，柱温为40 ℃，样品仓温度为15 ℃。采用多反应监测模式进行检测，用于定量分析的离子对为

177.0→161.2（丙泊酚）、

149.0→133.1（内标）。采用上述方法测定6例淋巴水肿患者血浆中丙泊酚的血浆浓度。

结果

丙泊酚检测质量浓度的线性范围为50～5 000 ng/mL（

＝0.995 0）；批内、批间精密度的RSD均不高于8.08%；空白血浆中无内源性干扰；无残留效应和稀释效应；提取回收率为89.80%～93.73%，基质效应为97.93%～101.73%；稳定性试验的RSD均小于3.27%。术中靶控输注2～30 min内，6例患者的丙泊酚血药浓度为1 865.3～6 056.2 ng/mL，停药后4～8 h内基本代谢完全。

结论

所建UPLC-MS/MS法可以在不进行衍生化的前提下，实现对丙泊酚的测定，样品前处理过程简便、快速，可用于淋巴水肿患者血浆样本中丙泊酚的血药浓度检测。

Abstract

OBJECTIVE

To establish a method for the determination of propofol concentration in human plasma and apply it in patients with lymphedema.

METHODS

The concentration of propofol was determined by UPLC-MS/MS after protein precipitation of plasma samples using thymol as internal standard. The sample was eluted on a Kinetex C

column with a mobile phase consisting of acetonitrile （A）-water （B） for gradient elution at the flow rate of 200 μL/min. The sample size was 5 μL， and the column temperature was set at 40 ℃. The sample chamber temperature was 15 ℃. Using multi-reaction monitoring mode， the ion pairs for quantitative analysis were

m/z

177.0→161.2 （propofol） and

m/z

149.0→133.1 （internal standard）， respectively. The above method was used to determine the plasma concentration of propofol in 6 patients with lymphedema.

RESULTS

The linear range of propofol was 50-5 000 ng/mL （

＝0.995 0）. RSDs of within- and between-batch precision were not more than 8.08%； no endogenous interference， carryover effect， or dilution effect was observed in blank plasma. The extraction recovery ranged from 89.80% to 93.73%， and matrix effects were within the range of 97.93%-101.73%. RSDs of the stability test were all lower than 3.27%. During intraoperative TCI 2-30 min， the plasma concentration of propofol in 6 patients was maintained in the range of 1 865.3-6 056.2 ng/mL， and the propofol was almost excreted within 4-8 h after operation.

CONCLUSIONS

The established UPLC-MS/MS method in this study can achieve the determination of propofol and a simple and fast sample pretreatment process without derivatization； it is proved to be suitable for the concentration monitoring of propofol in plasma samples of patients with lymphedema.

关键词

超高效液相-串联质谱法丙泊酚淋巴水肿血药浓度

Keywords

propofollymphedemaplasma concentration

references

姚晶曼，李元海. 依托咪酯联合丙泊酚用于老年患者无痛气管镜诊疗的临床观察[J]. 中国药房，2020，31（24）：3031-3035.

YAO J M，LI Y H. Clinical observation of the application of etomidate combined with propofol in diagnosis and treatment of elderly patients with painless tracheoscope[J]. China Pharm，2020，31（24）：3031-3035.

WALSH C T. Propofol：milk of amnesia[J]. Cell，2018，175（1）：10-13.

顾庆玲，黄民，李嘉丽. 丙泊酚药物不良反应潜在机制的研究现状[J]. 中国临床药理学杂志，2023，39（12）：1815-1819.

GU Q L，HUANG M，LI J L. Research status on the potential mechanisms of propofol adverse reactions[J]. Chin J Clin Pharmacol，2023，39（12）：1815-1819.

SINGH A，ANJANKAR A P. Propofol-related infusion syndrome：a clinical review[J]. Cureus，2022，14（10）：e30383.

邓景成，戴婷婷，曹卫刚. 淋巴水肿治疗的进展[J]. 组织工程与重建外科杂志，2016，12（1）：52-55.

DENG J C，DAI T T，CAO W G. Research progress of clinical therapy for lymphedema[J]. J Tissue Eng Reconstr Surg，2016，12（1）：52-55.

TIWARI P，CORIDDI M，SALANI R，et al. Breast and gynecologic cancer-related extremity lymphedema：a review of diagnostic modalities and management options[J]. World J Surg Oncol，2013，11：237.

BROUILLARD P，WITTE M H，ERICKSON R P，et al. Primary lymphoedema[J]. Nat Rev Dis Primers，2021，7（1）：77.

GREENE A K，GOSS J A. Diagnosis and staging of lymphedema[J]. Semin Plast Surg，2018，32（1）：12-16.

闫贺磊，刘勇，李果. 头颈部淋巴水肿的诊治进展[J]. 中国耳鼻咽喉颅底外科杂志，2023，29（2）：80-84.

YAN H L，LIU Y，LI G. Progress in diagnosis and treatment of head and neck lymphedema[J]. Chin J Otorhinolaryngol Skull Base Surg，2023，29（2）：80-84.

BAKER M T，NAGUIB M. Propofol：the challenges of formulation[J]. Anesthesiology，2005，103（4）：860-876.

郭田甜，尉洁，张帆，等. 丙泊酚及其代谢物在大鼠体内的动态分布[J]. 中国法医学杂志，2019，34（3）：213-217.

GUO T T，WEI J，ZHANG F，et al. Dynamic distribution of propofol and its metabolites in rats[J]. Chin J Forensic Med，2019，34（3）：213-217.

VAIANO F，SERPELLONI G，FOCARDI M，et al. LC-MS/MS and GC-MS methods in propofol detection：eva-luation of the two analytical procedures[J]. Forensic Sci Int，2015，256：1-6.

谭璐，杨翃，董瑞，等. 超高效液相色谱法监测重症脑损伤患者血浆中丙泊酚的血药浓度[J]. 中国医院药学杂志，2018，38（23）：2397-2401.

TAN L，YANG H，DONG R，et al. Monitoring the plasma concentration of propofol in intensive care unit patients with severe brain injury by UPLC[J]. Chin J Hosp Pharm，2018，38（23）：2397-2401.

SEGER C，SALZMANN L. After another decade：LC-MS/MS became routine in clinical diagnostics[J]. Clin Biochem，2020，82：2-11.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Research progress in therapy drug monitoring of levetiracetam

RP-HPLC-荧光法测定NM394 血药浓度及回归方程的选择

HPLC法测定人血清中左乙拉西坦的浓度

左卡尼汀对扩张型心肌病患者体内卡尼汀群的代谢影响

10-羟基喜树碱经2 种途径给药后在大鼠动、静脉血浆中的分布

Related Author

GAO Chang

NI Bin

CHEN Fanghui

GUO Chunyu

WEI Guilin

邵婷玑乔华廖明琪常威李丹马海忠李玲

李惠芬张玉琴刘彦

李盛楠荆凡波夏蕴秋郭琳琳孙振龙韩志武隋忠国王春波

Related Institution

Dept. of Pharmacy， the First Affiliated Hospital of Gannan Medical University

Ganzhou Key Laboratory of Immunotherapeutic Drugs Developing for Childhood Leukemia

Address：8/F,129 Daping Main Street,Yuzhong District,Chongqing 400042,P.R.China
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰