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Effects and mechanism of polysaccharides from
Hedyotis diffusa on isoniazid-induced liver injury- ZHONGGUO YAOFANG Vol. 35, Issue 6, Pages: 665-670(2024)
- 作者机构：
1.山东中医药大学药学院，济南 250355
2.山东中医药大学附属医院儿科，济南 250011
3.山东中医药大学附属医院药学部，济南 250011
- 作者简介：
- 基金信息：
DOI：10.6039/j.issn.1001-0408.2024.06.05
CLC： R965;R285Published：30 March 2024，
Received：21 August 2023，
Revised：29 February 2024，
扫 描 看 全 文
庄秀萍,李莉,陈超等.白花蛇舌草多糖对异烟肼致肝损伤的影响及机制 Δ[J].中国药房,2024,35(06):665-670.
ZHUANG Xiuping,LI Li,CHEN Chao,et al.Effects and mechanism of polysaccharides from Hedyotis diffusa on isoniazid-induced liver injury[J].ZHONGGUO YAOFANG,2024,35(06):665-670.
庄秀萍,李莉,陈超等.白花蛇舌草多糖对异烟肼致肝损伤的影响及机制 Δ[J].中国药房,2024,35(06):665-670. DOI： 10.6039/j.issn.1001-0408.2024.06.05.
ZHUANG Xiuping,LI Li,CHEN Chao,et al.Effects and mechanism of polysaccharides from Hedyotis diffusa on isoniazid-induced liver injury[J].ZHONGGUO YAOFANG,2024,35(06):665-670. DOI： 10.6039/j.issn.1001-0408.2024.06.05.
摘要
目的
2
探讨白花蛇舌草多糖（HDP）对异烟肼致肝损伤的影响及机制。
方法
2
以正常发育的具有肝脏特异性荧光的转基因斑马鱼为对象，分为正常组、模型组（4 mmol/L异烟肼）、HDP低浓度组（4 mmol/L异烟肼+50 mg/mL HDP）和HDP高浓度组（4 mmol/L异烟肼+100 mg/mL HDP），分组处理后观察斑马鱼肝脏荧光面积、荧光强度以及肝组织病理变化。以人肝L02细胞为对象，分为正常组、模型组（4 mmol/L异烟肼）、HDP低浓度组（4 mmol/L异烟肼+2 mg/mL HDP）和HDP高浓度组（4 mmol/L异烟肼+ 4 mg/mL HDP），分组处理后检测细胞存活情况，细胞上清液中丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）水平及细胞裂解液中谷胱甘肽（GSH）含量，沉默信息调节因子1（Sirt1）、核转录因子红系2相关因子2（Nrf2）、血红素加氧酶1（HO-1）、醌氧化还原酶1（NQO1）蛋白的表达水平。
结果
2
与模型组比较，HDP低、高浓度组斑马鱼肝脏荧光面积和荧光强度（HDP低浓度组除外）均显著增加（
P
＜0.05或
P
＜0.01），肝损伤坏死特征均有不同程度改善。与模型组比较，HDP低、高浓度组L02细胞存活率，GSH含量（HDP低浓度组除外），Sirt1（HDP低浓度组除外）、Nrf2、NQO1、HO-1（HDP低浓度组除外）蛋白表达水平均显著升高（
P
＜0.05或
P
＜0.01）；ALT、AST（HDP低浓度组除外）水平均显著降低（
P
＜0.05）；存活细胞数量均明显增加，损伤或死亡细胞数量均明显减少。
结论
2
HDP对异烟肼诱导的肝损伤具有一定的改善作用，其机制可能与激活Sirt1/Nrf2信号通路、改善线粒体功能、提高抗氧化能力相关。
Abstract
OBJECTIVE
2
To investigate the effects and mechanism of polysaccharides from
Hedyotis diffusa
（HDP） on isoniazid （INH）-induced liver injury.
METHODS
2
Healthy transgenic zebrafish with liver-specific fluorescence were divided into normal group， model group （4 mmol/L INH）， HDP low-concentration group （4 mmol/L INH+50 mg/mL HDP） and HDP high-concentration group （4 mmol/L INH+100 mg/mL HDP）. After grouping treating， the liver fluorescence area， fluorescence intensity and pathological changes of liver tissue were observed. Human liver L02 cells were divided into normal group， model group （4 mmol/L INH）， HDP low-concentration group （4 mmol/L INH+2 mg/mL HDP）， and HDP high-concentration group （4 mmol/L INH + 4 mg/mL HDP）. After grouping treating， the cell viability was detected， and the levels of alanine transaminase （ALT）， aspartate transaminase （AST）， and the content of glutathione （GSH） as well as the expression levels of silent information regulator 1 （Sirt1）， nuclear factor-erythroid 2-related factor 2 （Nrf2）， heme oxygenase-1 （HO-1） and quinone oxidoreductase 1 （NQO1） proteins were detected.
RESULTS
2
Compared with the model group， the HDP low- and high-concentration groups showed varying degrees of increase in the fluorescence area and fluorescence intensity （except for HDP low-concentration group） of zebrafish liver （
P
＜0.05 or
P
＜0.01）， and the characteristics of liver injury and necrosis had been improved to varying degrees. Compared with model group， the survival rate of L02 cells， the content of GSH （except for HDP low-concentration group）， the protein expression levels of Sirt1 （except for HDP low-concentration group）， Nrf2， NQO1， HO-1 （except for HDP low-concentration group） were significantly increased in HDP low- and high-concentration groups （
P
＜0.05 or
P
＜0.01）， and the levels of ALT and AST （except for HDP low-concentration group） were significantly decreased （
P
＜0.05）； the number of survival cells significantly increased， while the number of damaged or dead cells significantly decreased.
CONCLUSIONS
2
HDP has a potential protective effect against INH-induced liver injury， the mechanism of which may be associated with activating Sirt1/Nrf2 signaling pathway， improving mitochondrial function and enhancing antioxidant capacity.
关键词
白花蛇舌草多糖异烟肼肝损伤斑马鱼人肝L02细胞Sirt1/Nrf2信号通路
Keywords
isoniazidliver injuryzebrafishhuman liver L02 cellSirt1/Nrf2 signaling pathway
references
ANDRADE R J，CHALASANI N，BJÖRNSSON E S，et al. Drug-induced liver injury[J]. Nat Rev Dis Primers，2019，5（1）：58.
BJÖRNSSON E S. Epidemiology，predisposing factors，and outcomes of drug-induced liver injury[J]. Clin Liver Dis，2020，24（1）：1-10.
SHEN T，LIU Y X，SHANG J，et al. Incidence and etio-logy of drug-induced liver injury in mainland China[J]. Gastroenterology，2019，156（8）：2230-2241.
赵科，谭婉莹，向菊芳，等. 正交设计探讨一线抗结核药物致小鼠肝损伤相互作用研究[J]. 中药药理与临床，2022，38（2）：185-190.
ZHAO K，TAN W Y，XIANG J F，et al. Orthogonal design to investigate the interaction of first-line anti-tuberculosis drugs on liver injury in mice[J]. Pharmacol Clin Chin Mater Med，2022，38（2）：185-190.
中华医学会结核病学分会儿童结核病专业委员会，中国研究型医院学会结核病学专业委员会，国家呼吸系统疾病临床医学研究中心，等. 儿童结核性脑膜炎诊断专家共识[J]. 中华实用儿科临床杂志，2022，8（7）：497-501.
Pediatric Tuberculosis Speciality Committees of Chinese Society of Tuberculosis，Chinese Medical Association，Tuberculosis Speciality Committees of Chinese Research Hospital Association，National Clinical Research Center for Respiratory Diseases，et al. Expert consensus on the diag-nosis of tuberculous meningitis in children[J]. Chin J Appl Clin Pediatr，2022，8（7）：497-501.
VILLANUEVA-PAZ M，MORÁN L，LÓPEZ-ALCÁNTARA N，et al. Oxidative stress in drug-induced liver injury（DILI）：from mechanisms to biomarkers for use in clinical practice[J]. Antioxidants，2021，10（3）：390.
ZHUANG X P，LI L，LIU T Y，et al. Mechanisms of isoniazid and rifampicin-induced liver injury and the effects of natural medicinal ingredients：a review[J]. Front Pharmacol，2022，13：1037814.
王长玲，江振洲，张陆勇，等. 基于SIRT1治疗胆汁淤积性肝损伤的作用机制及药物研究进展[J]. 军事医学，2020，44（5）：390-395.
WANG C L，JIANG Z Z，ZHANG L Y，et al. Research progress in mechanisms of action and drugs in SIRT1-based treatment of cholestatic liver injury[J]. Mil Med Sci，2020，44（5）：390-395.
王信，李莉，张瑞，等. 基于指纹图谱和网络药理学的白花蛇舌草质量标志物预测分析[J]. 中国医院药学杂志，2022，42（16）：1633-1641.
WANG X，LI L，ZHANG R，et al. Predictive analysis of quality markers of Oldenlandia diffusa（Willd.）Roxb based on fingerprint and network pharmacology[J]. Chin J Hosp Pharm，2022，42（16）：1633-1641.
ZHANG R，MA C J，WEI Y L，et al. Isolation，purification，structural characteristics，pharmacological activities，and combined action of Hedyotis diffusa polysaccharides：a review[J]. Int J Biol Macromol，2021，183：119-131.
马河，程艳琳，张金杰，等. 白花蛇舌草总多糖的分离纯化、结构鉴定及初步免疫活性分析[J]. 中国实验方剂学杂志，2014，20（22）：37-40.
MA H，CHENG Y L，ZHANG J J，et al. Effect of preliminary immune activity and structural identification of a polysaccharide extracted from Oldenlandia diffusa[J]. Chin J Exp Tradit Med Formulae，2014，20（22）：37-40.
宋奇，杨杰，鲁瑶，等. 基于硫酸-苯酚法的可溶性糖高通量测定方法及其应用[J]. 食品安全质量检测学报，2022，13（5）：1480-1487.
SONG Q，YANG J，LU Y，et al. High-throughput method for determining soluble sugar based on sulfuric acid-phenol method and its application[J]. J Food Saf Qual，2022，13（5）：1480-1487.
JIA Z L，CEN J，WANG J B，et al. Mechanism of isoniazid-induced hepatotoxicity in zebrafish larvae：activation of ROS-mediated ERS，apoptosis and the Nrf2 pathway[J]. Chemosphere，2019，227：541-550.
VERMA A K，YADAV A，DEWANGAN J，et al. Isoniazid prevents Nrf2 translocation by inhibiting ERK1 pho-sphorylation and induces oxidative stress and apoptosis[J]. Redox Biol，2015，6：80-92.
粟仑，代立娟，黄如，等. SIRT1在肝损伤中作用的研究进展[J]. 广东化工，2022，49（1）：119-120，118.
SU L，DAI L J，HUANG R，et al. Research progress of SIRT1 in liver injury[J]. Guangdong Chem Ind，2022，49（1）：119-120，118.
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