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1.江西中医药大学现代中药制剂教育部重点实验室,南昌 330004
2.江西中医药大学附属医院科研科,南昌 330004
Received:26 September 2023,
Revised:2024-03-20,
Accepted:20 March 2024,
Published:15 April 2024
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管咏梅,陶颖,邹成玉,等.苦参-黄柏药对的提取工艺研究[J].中国药房,2024,35(07):793-800.
GUAN Yongmei,TAO Ying,ZOU Chengyu,et al.Study on extraction technology of Sophora flavescens-Phellodendron chinense drug pair[J].ZHONGGUO YAOFANG,2024,35(07):793-800.
管咏梅,陶颖,邹成玉,等.苦参-黄柏药对的提取工艺研究[J].中国药房,2024,35(07):793-800. DOI: 10.6039/j.issn.1001-0408.2024.07.05.
GUAN Yongmei,TAO Ying,ZOU Chengyu,et al.Study on extraction technology of Sophora flavescens-Phellodendron chinense drug pair[J].ZHONGGUO YAOFANG,2024,35(07):793-800. DOI: 10.6039/j.issn.1001-0408.2024.07.05.
目的
2
研究苦参-黄柏药对的提取工艺,为治疗肛肠疾病的新药开发提供参考依据。
方法
2
以苦参总碱(苦参碱+氧化苦参碱)含量、盐酸小檗碱含量、总黄酮含量及浸膏得率为评价指标,采用层次分析法-熵权法计算各指标权重系数,结合Box-Behnken设计-响应面法研究苦参-黄柏药对的最佳提取工艺并进行验证。
结果
2
苦参-黄柏药对的最优提取工艺为以12倍量58%乙醇浸泡30 min后提取2次,每次120 min。验证实验结果与预测值的相对误差为1.88%。
结论
2
所得提取工艺稳定、可行,可为苦参-黄柏药对的进一步应用与新药开发提供参考。
OBJECTIVE
2
To study the extraction technology of
Sophora flavescens
-
Phellodendron chinense
drug pair and provide a reference for the development of new drugs for the treatment of anorectal diseases.
METHODS
2
Using the contents of total alkaloids of
S. flavescens
(matrine+oxymatrine), berberine hydrochloride and total flavonoid, and extract yield as evaluation indicators, analytic hierarchy process-entropy weight method was used to calculate the weight coefficient of each indicator, and was combined with Box-Behnken design-response surface method to study the extraction technology of
S. flavescens
-
P. chinense
drug pair and verify it.
RESULTS
2
The optimal extraction technology of
S. flavescens
-
P. chinense
drug pair was immersed in 12-fold amount of 58% ethanol for 30 minutes and extracted twice, each time for 120 minutes. The relative error between the verification experimental results and the predicted value was 1.88%.
CONCLUSIONS
2
The obtained extraction technology is stable and feasible and ca
n provide reference for the application of
S. flavescens
-
P. chinense
drug pair and development of new drugs.
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