Differential component analysis between Fructus Tritici Levis and <italic style="font-style: italic">Triticum aestivum</italic> based on qualitative and quantitative methods

LI Xuejiao; HU Yu; CHEN Yun; SHANG Juan; LI Zhenyang; FENG Yunhua; ZOU Jiandong; YAO Weifeng; LU Su; XU Meijuan

doi:10.6039/j.issn.1001-0408.2024.11.03

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Differential component analysis between Fructus Tritici Levis and Triticum aestivum based on qualitative and quantitative methods

更新时间：2024-06-11

- Differential component analysis between Fructus Tritici Levis and Triticum aestivum based on qualitative and quantitative methods
- ZHONGGUO YAOFANG Vol. 35, Issue 11, Pages: 1296-1302(2024)
- 作者机构：
  
  1.南京中医药大学附属医院，南京　210029
  2.南京中医药大学第一临床医学院，南京　210023
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.11.03
  CLC： R284;R917
- Published：15 June 2024，
  
  Received：19 January 2024，
  
  Revised：08 May 2024，
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李雪娇,胡雨,陈赟等.基于定性、定量方法的浮小麦与小麦差异成分分析 ^Δ[J].中国药房,2024,35(11):1296-1302.

LI Xuejiao,HU Yu,CHEN Yun,et al.Differential component analysis between Fructus Tritici Levis and Triticum aestivum based on qualitative and quantitative methods[J].ZHONGGUO YAOFANG,2024,35(11):1296-1302.
李雪娇,胡雨,陈赟等.基于定性、定量方法的浮小麦与小麦差异成分分析 ^Δ[J].中国药房,2024,35(11):1296-1302. DOI： 10.6039/j.issn.1001-0408.2024.11.03.

LI Xuejiao,HU Yu,CHEN Yun,et al.Differential component analysis between Fructus Tritici Levis and Triticum aestivum based on qualitative and quantitative methods[J].ZHONGGUO YAOFANG,2024,35(11):1296-1302. DOI： 10.6039/j.issn.1001-0408.2024.11.03.

摘要

目的

分析浮小麦和小麦的成分差异，为二者的鉴定及质量控制提供参考。

方法

收集20批浮小麦和3批小麦药材，采用高效液相色谱法，利用《中药色谱指纹图谱相似度评价系统》（2012版）绘制指纹图谱并进行相似度评价，应用聚类分析（CA）、主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）对不同产地浮小麦及小麦样品进行差异分析，筛选差异成分，并测定浮小麦和小麦中6个指认成分的含量。

结果

浮小麦指纹图谱的相似度为0.928～0.996，小麦与浮小麦的相似度为0.761～0.773；在浮小麦和小麦中共标定19个共有峰，指认出了6个成分，分别为亚麻酸、亚油酸、5-十七烷基间苯二酚、5-十九烷基间苯二酚、5-二十一烷基间苯二酚和5-二十三烷基间苯二酚。CA、PCA结果显示，浮小麦和小麦能明显区分；产地为安徽的浮小麦的分布较为集中。OPLS-DA结果显示，亚麻酸、亚油酸及其他6个未知化合物为浮小麦和小麦的差异成分。浮小麦中上述6个指认成分的平均含量分别为0.100 9、1.094 0、0.005 1、0.030 9、0.098 2、0.024 8 mg/g，且浮小麦中亚麻酸、亚油酸的含量均显著高于小麦（

＜0.05）。

结论

所建定性、定量测定方法操作简单、结果可靠，可用于浮小麦和小麦的鉴别及质量评价。亚麻酸、亚油酸等差异成分可为浮小麦和小麦的鉴别及药效学差异研究提供线索。

Abstract

OBJECTIVE

To analyze the compositional differences between Fructus Tritici Levis and

Triticum aestivum

， and to provide reference for identification and quality control of both.

METHODS

Twenty batches of Fructus Tritici Levis and three batches of

T. aestivum

were collected， and their fingerprints were acquired by high-performance liquid chromatography and the similarities were evaluated by

the Evaluation System of Similarity of Chromatographic Fingerprints of Traditional Chinese Medicine

（2012 version）. Cluster analysis （CA）， principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis （OPLS-DA） were performed to analyze the difference of Fructus Tritici Levis and

T. aestivum

from different regions， and the differential components were screened. The contents of the six identified components in Fructus Tritici Levis and

T. aestivum

were determined.

RESULTS

The similarities of the fingerprints of Fructus Tritici Levis ranged from 0.928 to 0.996， and the relative similarities of

T. aestivum

with Fructus Tritici Levis ranged from 0.761 to 0.773. A total of 19 common peaks were calibrated， and six components including linolenic acid， linoleic acid， 5-heptadecylresorcinol， 5-nonadodecylresorcinol， 5-heneicosylresorcinol， and 5-tricosylresorcinol were identified. The results of CA and PCA showed that Fructus Tritici Levis and

T. aestivum

could be clearly distinguished； the distribution of Fructus Tritici Levis from Anhui province was relatively concentrated. The results of OPLS-DA showed that linolenic acid， linoleic acid， and other six unknown compounds were the differential components between Fructus Tritici Levis and

T. aestivum

. The average contents of the six identified components in Fructus Tritici Levis were 0.100 9， 1.094 0， 0.005 1， 0.030 9， 0.098 2， and 0.024 8 mg/g， respectively； the contents of linolenic acid and linoleic acid in Fructus Tritici Levis were significantly higher than those in

T. aestivum

（

＜0.05）.

CONCLUSIONS

The established qualitative and quantitative methods are simple and reliable， and can be used for the identification and quality evaluation of Fructus Tritici Levis and

T. aestivum

. The identified differential components， such as linolenic acid and linoleic acid， can also provide clues for the differentiation and pharmacological study of Fructus Tritici Levis and

T. aestivum

关键词

浮小麦小麦指纹图谱含量测定化学计量学分析差异成分高效液相色谱法

Keywords

Triticum aestivumfingerprintcontent determinationchemometrics analysisdifferential componentsHPLC

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Differential component analysis between Fructus Tritici Levis and Triticum aestivum based on qualitative and quantitative methods

DOI：10.6039/j.issn.1001-0408.2024.11.03

摘要

Abstract

关键词

Keywords

references