Effect of ethyl acetate extract from <italic style="font-style: italic">Mimosa pudica</italic> root on acute myeloid leukemia in mice

DENG Wanrou; LI Huimin; ZENG Yuanning; WANG Qiuhong

doi:10.6039/j.issn.1001-0408.2024.11.06

您当前的位置：

首页 >

文章列表页 >

Effect of ethyl acetate extract from Mimosa pudica root on acute myeloid leukemia in mice

更新时间：2024-06-11

- Effect of ethyl acetate extract from Mimosa pudica root on acute myeloid leukemia in mice
- ZHONGGUO YAOFANG Vol. 35, Issue 11, Pages: 1315-1319(2024)
- 作者机构：
  
  1.广东药科大学中药学院/广东省中药饮片规范化炮制工程技术研究中心，广州　510006
  2.黑龙江中医药大学教育部北药基础与应用研究重点实验室/黑龙江省中药及天然药物药效物质基础研究重点实验室，哈尔滨　150040
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.11.06
  CLC： R965;R285
- Published：15 June 2024，
  
  Received：27 October 2023，
  
  Revised：28 March 2024，
扫描看全文
邓婉柔,李慧敏,曾元宁等.含羞草根乙酸乙酯部位对急性髓系白血病小鼠的影响 ^Δ[J].中国药房,2024,35(11):1315-1319.

DENG Wanrou,LI Huimin,ZENG Yuanning,et al.Effect of ethyl acetate extract from Mimosa pudica root on acute myeloid leukemia in mice[J].ZHONGGUO YAOFANG,2024,35(11):1315-1319.
邓婉柔,李慧敏,曾元宁等.含羞草根乙酸乙酯部位对急性髓系白血病小鼠的影响 ^Δ[J].中国药房,2024,35(11):1315-1319. DOI： 10.6039/j.issn.1001-0408.2024.11.06.

DENG Wanrou,LI Huimin,ZENG Yuanning,et al.Effect of ethyl acetate extract from Mimosa pudica root on acute myeloid leukemia in mice[J].ZHONGGUO YAOFANG,2024,35(11):1315-1319. DOI： 10.6039/j.issn.1001-0408.2024.11.06.

摘要

目的

研究含羞草根乙酸乙酯部位（简称为乙酸乙酯部位）对急性髓系白血病小鼠的影响。

方法

以不同质量浓度（0.062 5、0.125、0.25、0.5 mg/mL）乙酸乙酯部位作用于急性粒-单核细胞白血病细胞WEHI-3，考察其对该细胞活力的影响。将50只BALB/C小鼠随机分为空白对照组、模型组、阳性对照组（5-氟尿嘧啶，13 mg/kg）和乙酸乙酯部位低、高剂量组（50、200 mg/kg），每组10只。除空白对照组外，其余各组小鼠均腹腔注射WEHI-3细胞悬液进行造模，并在造模第2天起灌胃相应药物/水，每天1次，连续14 d。末次给药后，检测小鼠肝、脾指数，并进行肝组织病理学形态观察和血液学分析以及白细胞分化检测；检测小鼠血清中细胞因子[白细胞介素2（IL-2）、IL-3、干扰素γ（IFN-γ）、肿瘤坏死因子α（TNF-α）]水平；检测小鼠全血中白细胞表面标志物[分化簇3（CD3）、CD19、CD11b、分化簇107b（即Mac-3）]抗体水平。

结果

经0.062 5～0.5 mg/mL的乙酸乙酯部位作用后，WEHI-3细胞增殖抑

制率均显著升高（

＜0.05）。经高剂量的乙酸乙酯部位干预后，小鼠的肝、脾指数，血清中TNF-α水平，全血中白细胞表面CD11b、Mac-3抗体水平均显著降低（

＜0.05），血清中IL-2、IL-3、IFN-γ水平和全血中白细胞表面CD3、CD19抗体水平均显著升高（

＜0.05）；肝实质偶有淋巴细胞浸润，几乎无炎症细胞浸润；血液学指标改善且白细胞分化减弱。

结论

含羞草根乙酸乙酯部位可抑制WEHI-3细胞增殖，改善急性髓系白血病小鼠症状，其作用机制与增强机体免疫功能有关。

Abstract

OBJECTIVE

To study the inhibitory effect of ethyl acetate extract from

Mimosa pudica

root （ethyl acetate extract for short） on acute myeloid leukemia in mice.

METHODS

Different concentrations of ethyl acetate extract （0.062 5， 0.125， 0.25， 0.5 mg/mL） were used to treat acute myelomonocytic leukemia cell lines WEHI-3， and their effects on cell viability were investigated. Fifty BALB/C mice were randomly divided into blank control group， model group， positive control group （5-fluorouracil， 13 mg/kg）， and ethyl acetate extract low-dose and high-dose groups （50， 200 mg/kg）， with 10 mice in each group. Except for the blank control group， the leukemia model was constructed by intraperitoneal injection of WEHI-3 cells in other groups， and from the second day of modeling， corresponding drugs/water were orally administered once a day for 14 consecutive days. After the last administration， the liver and spleen indexes of mice were measured， and liver tissue pathological morphology observation， hematological analysis， and white blood cell differentiation detection were performed； the levels of cytokine [interleukin-2 （IL-2）， IL-3， interferon-γ （IFN-γ）， tumor necrosis factor-α （TNF-α）] in serum were determined； the levels of leukocyte surface markers [cluster of differentiation 3 （CD3）， CD19， CD11b， CD107b （Mac-3）] in whole blood were all detected.

RESULTS

After treated with 0.062 5-0.5 mg/mL ethyl acetate， the inhibition rate of cell proliferation were increased significantly （

＜0.05）. After intervention with high-d

ose ethyl acetate， the liver and spleen index， serum level of TNF-α， the levels of CD11b and Mac-3 in blood were significantly reduced （

＜0.05）， while serum levels of IL-2， IL-3 and IFN-γ， and the levels of CD3 and CD19 in blood were increased significantly （

＜0.05）. Occasional lymphocyte infiltration was present in the liver parenchyma， with almost no infiltration of inflammatory cells； hematology improvement and weakened white blood cell differentiation were found.

CONCLUSIONS

The ethyl acetate extract of

M. pudica

root can inhibit the proliferation of WEHI-3 cells， and improve symptoms in acute myeloid leukemia mice， the mechanism of which may be associated with enhancing the immune function.

关键词

含羞草根乙酸乙酯部位急性髓系白血病白细胞分化免疫功能

Keywords

ethyl acetate extractacute myeloid leukemiawhite blood cell differentiationimmune function

references

张晓丹，周永明，严静贤. 中医药治疗急性髓系白血病的研究进展[J]. 中国中医急症，2020，29（5）：925-929.

ZHANG X D，ZHOU Y M，YAN J X. Research progress on treatment of acute myeloid leukemia with traditional Chinese medicine[J]. J Emerg Tradit Chin Med，2020，29（5）：925-929.

吕国庆，张肖肖，吴隼，等. 急性单核细胞白血病的临床特点及预后分析[J]. 现代肿瘤医学，2022，30（17）：3199-3203.

LYU G Q，ZHANG X X，WU S，et al. Analysis of clinical features and prognostic factors of acute monocytic leukemia[J]. J Mod Oncol，2022，30（17）：3199-3203.

王卫东，何小花，杨庆敏，等. 治疗急性髓系白血病的中药活性成分研究进展[J]. 时珍国医国药，2021，32（6）：1444-1449.

WANG W D，HE X H，YANG Q M，et al. Research pro- gress on active components of traditional Chinese medicine for treating acute myeloid leukemia[J]. Lishizhen Med Mater Med Res，2021，32（6）：1444-1449.

PINHEIRO FERREIRA P M，DRUMOND R R，SILVA J D N，et al. Chemotherapeutic and safety profile of a fraction from Mimosa caesalpiniifolia stem bark[J]. J Oncol，2021，2021：9031975.

BRITO L M，DE MORAES ALVES M M，SOUZA A C，et al. Selective in vitro antileishmanial activity of Mimosa caesalpiniifolia stem barks and its main constituent betulinic acid against Leishmania amazonensis[J]. S Afr N J Bot，2021，140：68-75.

YANG H L，THIYAGARAJAN V，LIAO J W，et al. Toona sinensis inhibits murine leukemia WEHI-3 cells and promotes immune response in vivo[J]. Integr Cancer Ther，2017，16（3）：308-318.

马嘉鑫，刘永忠，李文宏，等. 中药逆转白血病多药耐药机制研究进展[J]. 江西中医药大学学报，2022，34（4）：120-124.

MA J X，LIU Y Z，LI W H，et al. Research progress on reversing multidrug resistance of leukemia with traditional Chinese medicine[J]. J Jiangxi Univ Chin Med，2022，34（4）：120-124.

WHITELEY A E，PRICE T T，CANTELLI G，et al. Leukaemia：a model metastatic disease[J]. Nat Rev Cancer，2021，21（7）：461-475.

卢聪，吴耀辉，胡俊斌. 急性白血病患者早期危重症概述[J]. 临床血液学杂志，2024，37（3）：156-161.

LU C，WU Y H，HU J B. An overview of early critical illness in patients with acute leukemia[J]. J Clin Hematol，2024，37（3）：156-161.

杨莹，关红梅，王舒，等. 脑脊液与血清IGF-1、IGF-2、IGFBP-3、IL-2、IL-6、IL-8在急性淋巴细胞性白血病中的表达水平及意义[J]. 热带医学杂志，2020，20（11）：1438-1441.

YANG Y，GUAN H M，WANG S，et al. Expression and significance of cerebrospinal fluid and serum IGF-1，IGF-2，IGFBP-3，IL-2，IL-6，IL-8 in acute lymphocytic leukemia[J]. J Trop Med，2020，20（11）：1438-1441.

LOPATINA T，KONI M，GRANGE C，et al. IL-3 signalling in the tumour microenvironment shapes the immune response via tumour endothelial cell-derived extracellular vesicles[J]. Pharmacol Res，2022，179：106206.

张小梅，陈莉. 细胞因子IL-6、IL-10、TNF-α、IFN-γ在急性白血病患者感染中的诊断价值[J]. 中国处方药，2021，19（4）：143-145.

ZHANG X M，CHEN L. Diagnostic value of cytokines IL-6，IL-10，TNF-α and IFN-γ in acute leukemia patients with infection[J]. J China Prescr Drug，2021，19（4）：143-145.

成娟，马海珍，张豪. 急性白血病患者T、B淋巴细胞的检测与分析[J]. 中国实验血液学杂志，2019，27（2）：327-330.

CHENG J，MA H Z，ZHANG H. Detection and analysis of T lymphocyte subsets and B lymphocytes in patients with acute leukemia[J]. J Exp Hematol，2019，27（2）：327-330.

白会恩，闫理想，林旭，等. CD19在急性髓系白血病中的研究进展[J]. 疑难病杂志，2023，22（4）：441-444.

BAI H E，YAN L X，LIN X，et al. Research progress of CD19 in acute myeloid leukemia[J]. Chin J Difficult Complicat Cases，2023，22（4）：441-444.

杨翀，张毅. 嵌合抗原受体T细胞治疗恶性肿瘤的研究进展[J]. 天津药学，2023，35（2）：72-78.

YANG C，ZHANG Y. Research progress of chimeric antigen receptor T cell therapy in treating malignancies[J]. Tianjin Pharm，2023，35（2）：72-78.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Analysis of therapeutic effect of modified Yupingfeng powder combined with Yougui pill on COPD patients with deficiency of lung and kidney in remission period

适应原样药物刺五加及其化学成分在载人航天中的应用

肺癌患者术后在ICU应用右美托咪定对免疫功能的影响

枸杞多糖对肝癌模型小鼠肿瘤抑制与免疫功能的影响

四生汤联合补中益气汤对脾虚患者胃肠道和免疫功能的影响

Related Author

Bing QIU

Dezhi HONG

Jingjing ZENG

Xiaohua HU

Yuhuan HUANG

刘军莲高建义李勇枝盖宇清郑石英

周明明袁建国周艳红张　宁

肖佩玉万正兰黄际薇

Related Institution

Dept. of Internal Medicine， Fuzhou Hospital of Traditional Chinese Medicine

Center for Treatment and Prevention， the Affiliated Hospital of Jiangxi University of Chinese Medicine

Dept. of Cardiovascular Medicine， Jiangxi Provincial People’s Hospital

Address：8th Floor, Sihuan Building, No. 129 Daping Main Street, Yuzhong District, Chongqing, China Postal code：400042
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Effect of ethyl acetate extract from Mimosa pudica root on acute myeloid leukemia in mice

DOI：10.6039/j.issn.1001-0408.2024.11.06

摘要

Abstract

关键词

Keywords

references