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Effect and mechanism of dioscin on renal injury in septic rats
- ZHONGGUO YAOFANG Vol. 35, Issue 11, Pages: 1334-1338(2024)
- 作者机构：
成都大学附属医院急诊科，成都 610081
- 作者简介：
- 基金信息：
DOI：10.6039/j.issn.1001-0408.2024.11.09
CLC： R965;R285.5Published：15 June 2024，
Received：28 December 2023，
Revised：11 April 2024，
扫 描 看 全 文
申翔,徐尚刚,黄洋辉等.薯蓣皂苷对脓毒症大鼠肾损伤的影响及机制 Δ[J].中国药房,2024,35(11):1334-1338.
SHEN Xiang,XU Shanggang,HUANG Yanghui,et al.Effect and mechanism of dioscin on renal injury in septic rats[J].ZHONGGUO YAOFANG,2024,35(11):1334-1338.
申翔,徐尚刚,黄洋辉等.薯蓣皂苷对脓毒症大鼠肾损伤的影响及机制 Δ[J].中国药房,2024,35(11):1334-1338. DOI： 10.6039/j.issn.1001-0408.2024.11.09.
SHEN Xiang,XU Shanggang,HUANG Yanghui,et al.Effect and mechanism of dioscin on renal injury in septic rats[J].ZHONGGUO YAOFANG,2024,35(11):1334-1338. DOI： 10.6039/j.issn.1001-0408.2024.11.09.
摘要
目的
2
探究薯蓣皂苷对脓毒症大鼠肾损伤的影响及可能机制。
方法
2
采用盲肠结扎和穿刺法建立脓毒症大鼠模型。将建模成功的60只大鼠按照随机数字表法分为模型组（0.5%羧甲基纤维素钠溶液），薯蓣皂苷低、中、高剂量组（30、60、120 mg/kg）和地塞米松组（阳性对照，10 mg/kg），每组12只；另取12只大鼠作为假手术组（0.5%羧甲基纤维素钠溶液）。造模15 min后，各组大鼠尾静脉注射给药/0.5%羧甲基纤维素钠溶液。给药24 h后，检测大鼠血清中肌酐（Cr）、尿素氮（BUN）、中性粒细胞明胶酶相关脂质运载蛋白（NGAL）、肾损伤分子1（KIM-1）、白细胞介素6（IL-6）、IL-1β、肿瘤坏死因子α（TNF-α）水平以及肾组织中丙二醛（MDA）水平、超氧化物歧化酶（SOD）活性和核因子E2相关因子2（Nrf2）、血红素加氧酶1（HO-1）、NOD样受体蛋白3（NLRP3）表达量，并观察肾组织病理形态学变化。
结果
2
与模型组比较，薯蓣皂苷低、中、高剂量组大鼠肾组织病理损伤明显改善，血清中Cr、BUN、NGAL、KIM-1、IL-6、IL-1β、TNF-α水平和肾组织中MDA水平、NLRP3蛋白表达量均显著降低（
P
＜0.05），肾组织中SOD活性和Nrf2、HO-1蛋白表达量均显著升高（
P
＜0.05），且薯蓣皂苷的作用具有剂量依赖性（
P
＜0.05）；薯蓣皂苷高剂量组和地塞米松组大鼠肾组织病理损伤情况相似，上述指标水平差异均无统计学意义（
P
＞0.05）。
结论
2
薯蓣皂苷可能是通过激活Nrf2/HO-1信号通路抑制NLRP3炎性小体，实现对炎症因子表达和氧化应激的抑制，从而发挥对脓毒症肾损伤的保护作用。
Abstract
OBJECTIVE
2
To investigate the effect of dioscin on renal injury in septic rats and its possible mechanism.
METHODS
2
The septic rat model was induced by using cecal ligation and puncture. Sixty model rats were randomly divided into model group （0.5% sodium carboxymethyl cellulose solution）， dioscin low-dose， medium-dose and high-dose groups （30， 60， 120 mg/kg） and dexamethasone group （positive control， 10 mg/kg）， with 12 rats per group； another 12 rats were selected as the sham operation group （0.5% sodium carboxymethyl cellulose solution）. After 15 minutes of modeling， rats in each group were injected with medicine/0.5% sodium carboxymethyl cellulose solution via the tail vein. Twenty-four hours after administration， the levels of creatinine （Cr）， blood urea nitrogen （BUN）， neutrophil gelatinase associated lipocalin （NGAL）， kidney injury molecule-1 （KIM-1）， interleukin 6 （IL-6）， IL-1β and tumor necrosis factor-α （TNF-α） in serum and malondialdehyde （MDA） in renal tissue， superoxide dismutase （SOD） activity and the protein expressions of nuclear factor E2-related factor 2 （Nrf2）， heme oxygenase-1 （HO-1）， NOD-like receptor protein 3 （NLRP3） were detected； renal histomorphology was observed.
RESULTS
2
Compared with model group， pathological injury of renal tissue was improved significantly in dioscin low-dose， medium-dose and high-dose groups； the levels of Cr， BUN， NGAL， KIM-1， IL-6， IL-1β and TNF-α in serum， MDA level and protein expression of NLRP3 in renal tissue were decreased significantly （
P
＜0.05）； SOD activity in renal tissue， protein expressions of Nrf2 and HO-1 were increased significantly （
P
＜0.05）， in a dose-dependent manner （
P
＜0.05）. The pathological damage of renal tissue in the dioscin high-dose group was similar to dexamethasone group， and there was no statistically sig
nificant difference in the levels of the above indicators （
P
＞0.05）.
CONCLUSIONS
2
Dioscin can activate the Nrf2/HO-1 signaling pathway to inhibit NLRP3 inflammasome， and realize the inhibition of inflammatory factors and oxidative stress， so as to protect the kidney injury in sepsis.
关键词
薯蓣皂苷Nrf2/HO-1信号通路NLRP3炎性小体脓毒症肾损伤
Keywords
Nrf2/HO-1 signaling pathwayNOD-like receptor protein 3 inflammasomesepsisrenal injury
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