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Study on the protective effect and mechanism of soybean isoflavones against threatened miscarriage model rats
- ZHONGGUO YAOFANG Vol. 35, Issue 12, Pages: 1482-1488(2024)
- 作者机构：
1.郑州卫生健康职业学院妇儿教研室， 河南 荥阳 450199
2.郑州大桥医院妇产科，郑州 450053
3.河南中医药大学第一附属医院妇产科，郑州 450003
- 作者简介：
- 基金信息：
DOI：10.6039/j.issn.1001-0408.2024.12.12
CLC： R965;R285Published：30 June 2024，
Received：08 November 2023，
Revised：22 March 2024，
扫 描 看 全 文
李三阳,金蓬勃,王秋红等.大豆异黄酮对先兆流产模型大鼠的保胎作用及机制研究 Δ[J].中国药房,2024,35(12):1482-1488.
LI Sanyang,JIN Pengbo,WANG Qiuhong,et al.Study on the protective effect and mechanism of soybean isoflavones against threatened miscarriage model rats[J].ZHONGGUO YAOFANG,2024,35(12):1482-1488.
李三阳,金蓬勃,王秋红等.大豆异黄酮对先兆流产模型大鼠的保胎作用及机制研究 Δ[J].中国药房,2024,35(12):1482-1488. DOI： 10.6039/j.issn.1001-0408.2024.12.12.
LI Sanyang,JIN Pengbo,WANG Qiuhong,et al.Study on the protective effect and mechanism of soybean isoflavones against threatened miscarriage model rats[J].ZHONGGUO YAOFANG,2024,35(12):1482-1488. DOI： 10.6039/j.issn.1001-0408.2024.12.12.
摘要
目的
2
研究大豆异黄酮对先兆流产大鼠的保胎作用及可能机制。
方法
2
取雌鼠促情，与雄鼠交配、妊娠后随机分为正常组（纯化水灌胃，
n
＝10）、模型组（纯化水灌胃，
n
＝9）、阳性对照药组（黄体酮4 mg/kg，肌内注射，
n
＝9）和大豆异黄酮低、中、高剂量组（25、50、100 mg/kg灌胃，
n
＝10）。除正常组外，其余各组大鼠均于孕8 d以米非司酮+米索前列醇灌胃建立先兆流产模型。各组大鼠分别于孕1～7 d、孕9～12 d给药/纯化水，每天1次。孕14 d时，计算各组大鼠的保胎率，检测大鼠血清中
β
-人绒毛膜促性腺激素（
β
-HCG）、孕酮（P）水平，观察大鼠胎盘与蜕膜组织病理形态学变化并检测其细胞凋亡指数，检测胎盘组织中凋亡相关因子（Fas）、凋亡相关因子配体（FasL）、增殖细胞核抗原（PCNA）和肝素结合表皮生长因子（HB-EGF） mRNA及其蛋白表达，检测蜕膜组织中Fas、PCNA和HB-EGF mRNA及其蛋白表达。
结果
2
模型组大鼠胎盘组织充血扩张明显，血管较少且形态不规则，可见大量严重基质水肿、炎症细胞浸润和铁胆黄素沉积。与模型组比较，大豆异黄酮各剂量组大鼠的保胎率，血清
β
-HCG、P水平，胎盘与蜕膜组织中PCNA、HB-EGF mRNA及其蛋白表达水平均显著升高（
P
＜0.05），病理学变化显著改善；胎盘与蜕膜组织细胞凋亡指数，胎盘组织中Fas、FasL mRNA及其蛋白表达水平，蜕膜组织中Fas mRNA及其蛋白表达水平均显著降低（
P
＜0.05），且大豆异黄酮的上述作用呈剂量依赖性（
P
＜0.05）。
结论
2
大豆异黄酮对先兆流产模型大鼠有保胎作用；其作用机制可能与下调母-胎界面Fas、FasL mRNA及其蛋白表达，上调PCNA、HB-EGF mRNA及其蛋白表达有关。
Abstract
OBJECTIVE
2
To study the protective effect and possible mechanism of soybean isoflavones against threatened miscarriage rats.
METHODS
2
Female mice were selected to promote estrus and mate with male mice. After pregnancy， they were randomly divided into normal group （purified water， i.g.，
n
＝10）， model group （purified water， i.g.，
n
＝9）， positive control drug group （progesterone 4 mg/kg， i.m.，
n
＝9）， low-， medium- and high-dose soybean isoflavone group
s （25， 50 and 100 mg/kg， i.g.，
n
＝10）. Except for the normal group， the rest were given mifepristone+misoprostol on the 8th day of pregnancy to establish threatened miscarriage model， and then given purified water or drugs， once a day， on days 1-7 and 9-12 of pregnancy， respectively. At 14 days of pregnancy， the rates of fetal protection were counted. Serum levels of
β
-human chorionic gonadotrophin （
β
-HCG） and progesterone （P） in rats were detected. Pathological and morphological changes in rat placenta and decidua tissues were observed， and the apoptosis indexes of cells were detected； mRNA and protein expressions of factor of apoptosis related （Fas）， factor of apoptosis related ligand （FasL）， proliferating cell nuclear antigen （PCNA） and heparin binding epidermal growth factor （HB-EGF） were determined in placenta tissues， and mRNA and protein expressions of Fas， PCNA and HB-EGF in decidua tissues were detected.
RESULTS
2
In the model group， the placental tissues of rats were hyperemia and dilatation， with fewer and irregular blood vessels； severe stromal edema， inflammatory cell infiltration and iron-choledrin deposition were observed. Compared with model group， the fetal survival rates， serum levels of
β
-HCG and P， the expressions of PCNA and HB-EGF mRNA and proteins in the placenta and decidua tissue of soybean isoflavone groups increased significantly （
P
＜0.05）， while pathological changes were improved significantly； cell apoptosis index in the placenta and decidua tissue， the expressions of Fas， FasL mRNA and proteins in the placenta and Fas mRNA and protein in the decidua tissue decreased significantly （
P
＜0.05）. The effect of soybean isoflavones was dose-dependent （
P
＜0.05）.
CONCLUSIONS
2
Soybean isoflavone has protective effect on threatened miscarriage， the mechanism of which is related to down-regulating the expressions of Fas and FasL mRNA and protein at the maternal-fetal interface， and up-regulating the expressions of mRNA and protein of PCNA and HB-EGF.
关键词
大豆异黄酮先兆流产母-胎界面凋亡相关因子凋亡相关因子配体增殖细胞核抗原肝素结合表皮生长因子
Keywords
threatened miscarriagematernal-fetal interfacefactor of apoptosis relatedfactor of apoptosis related ligandproliferating cell nuclear antigenheparin binding epidermal growth factor
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