Analysis of <italic style="font-style: italic">in vivo</italic> components and metabolism pathway in rats after intragastric administration of aqueous extract from <italic style="font-style: italic">Abrus cantoniensis</italic>

CHEN Kuikui; NONG Yuxin; GUO Zhonghui; TAN Yong; HUANG Hongting; LI Jinman; WEI Jinrui; WEI Zhiying; LIANG Jie

doi:10.6039/j.issn.1001-0408.2024.13.06

您当前的位置：

首页 >

文章列表页 >

Analysis of in vivo components and metabolism pathway in rats after intragastric administration of aqueous extract from Abrus cantoniensis

更新时间：2024-07-10

- Analysis of in vivo components and metabolism pathway in rats after intragastric administration of aqueous extract from Abrus cantoniensis
- ZHONGGUO YAOFANG Vol. 35, Issue 13, Pages: 1576-1581(2024)
- 作者机构：
  
  1.广西中医药大学药学院，南宁　530200
  2.广西高校中药民族药资源保护与利用重点实验室，南宁　530200
  3.广西高校中药提取纯化与质量分析重点实验室，南宁　530200
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.13.06
  CLC： R965;R917
- Published：15 July 2024，
  
  Received：22 January 2024，
  
  Revised：14 June 2024，
- 稿件说明：
扫描看全文
陈奎奎,农雨欣,郭忠会等.鸡骨草水提物灌胃大鼠后的体内成分及代谢途径分析 ^Δ[J].中国药房,2024,35(13):1576-1581.

CHEN Kuikui,NONG Yuxin,GUO Zhonghui,et al.Analysis of in vivo components and metabolism pathway in rats after intragastric administration of aqueous extract from Abrus cantoniensis[J].ZHONGGUO YAOFANG,2024,35(13):1576-1581.
陈奎奎,农雨欣,郭忠会等.鸡骨草水提物灌胃大鼠后的体内成分及代谢途径分析 ^Δ[J].中国药房,2024,35(13):1576-1581. DOI： 10.6039/j.issn.1001-0408.2024.13.06.

CHEN Kuikui,NONG Yuxin,GUO Zhonghui,et al.Analysis of in vivo components and metabolism pathway in rats after intragastric administration of aqueous extract from Abrus cantoniensis[J].ZHONGGUO YAOFANG,2024,35(13):1576-1581. DOI： 10.6039/j.issn.1001-0408.2024.13.06.

摘要

目的

分析鸡骨草水提物灌胃大鼠后的入血和入肝成分，并推测其可能的代谢途径，为鸡骨草的药效物质基础分析提供参考。

方法

将雄性SD大鼠随机分为鸡骨草给药组（0.63 g/kg，以生药量计）、空白组，分别单次灌胃相应药液/超纯水，收集各组大鼠的血浆和肝脏样品。采用超高效液相色谱-四极杆-飞行时间质谱（UPLC-Q-TOF/MS）技术对鸡骨草水提物化学成分及入血、入肝成分进行鉴定。

结果

从鸡骨草水提物中共鉴定了30个化学成分，主要为生物碱类、黄酮类、有机酸类和环烯醚萜类（如相思子碱、夏佛塔苷和异夏佛塔苷等）等。从血浆样品中鉴定了10个原型成分和9个代谢产物（如原儿茶酸脱羧化硫酸化代谢产物、对羟基苯甲酸还原化硫酸化代谢产物等），从肝脏样品中鉴定了6个原型成分和5个代谢产物（如对羟基苯甲酸硫酸化代谢产物、对羟基苯甲酸脱羧化硫酸化代谢产物等）。代谢途径主要包括羟基化、去甲基化、甲基化、硫酸化、葡萄糖醛酸化等。

结论

生物碱类、黄酮类、有机酸类是鸡骨草水提物入血和入肝的主要成分，主要涉及羟基化、去甲基化、硫酸化等代谢途径。

Abstract

OBJECTIVE

To analyze the chemical components

that were the absorbed in blood and liver tissue of rats after intragastric administration of aqueous extract from

Abrus cantoniensis

， and to speculate its possible metabolic pathways， providing reference for basic analysis of pharmacological substance in

A. cantoniensis

METHODS

Male SD rats were randomly divided into

A. cantoniensis

group （0.63 g/kg， calculated by crude drug） and blank group； they were given relevant drug solution/ultrapure water intragastrically. After a single dose， plasma and liver samples of rats in each group were collected. UPLC-Q-TOF/MS technology was used to identify chemical components that were absorbed in the blood and liver tissue of rats.

RESULTS

Totally， 30 chemical constituents were identified from the water extracts of

A. cantoniensis

， including alkaloids， flavonoids， organic acids， iridoids （such as L-abrine， schaftoside， isoshaftoside）. Ten prototype components and nine metabolites （such as decarboxylation and sulfation metabolites of protocatechuic acid， reduced sulfated metabolites of

-hydroxybenzoic acid） were identified from plasma samples； six prototype components and five metabolites （such as sulfated metabolites of

-hydroxybenzoic acid， decarboxylation and sulfation metabolites of

-hydroxybenzoic acid） were identified from liver samples. The main metabolic pathways included hydroxylation， demethylation， methylation， sulfation， glucuronidation， etc.

CONCLUSIONS

Alkaloids， flavonoids and organic acids are the main components of the aqueous extract from

A. cantoniensis

that are absorbed into the blood and liver， their metabolism mainly involves hydroxylation， demethylation， and sulfation.

关键词

鸡骨草血浆肝脏UPLC-Q-TOF/MS药源性成分

Keywords

plasmaliverUPLC-Q-TOF/MSdrug-derived ingredients

references

国家药典委员会. 中华人民共和国药典：一部[M]. 2020年版. 北京：中国医药科技出版社，2020：203.

National Pharmacopoeia Commission. Chinese pharmacopoeia：part Ⅰ [M]. 2020 edition. Beijing：China Medical Science and Technology Press，2020：203.

杨燕玲. 鸡骨草对APAP诱导的小鼠肝损伤的保护作用及机制研究[D]. 赣州：赣南医学院，2023.

YANG Y L. Protective effect and mechanism of Abrus cantoniensis on liver injury induced by APAP in mice[D]. Ganzhou：Gannan Medical University，2023.

林海佳，苏志恒，何颖，等. 鸡骨草提取物对对乙酰氨基酚诱导小鼠急性肝损伤保护作用的研究[J]. 广西医科大学学报，2023，40（5）：733-741.

LIN H J，SU Z H，HE Y，et al. Protective effect of Abrus cantoniensis Hance on acetaminophen-induced acute liver injury in mice[J]. J Guangxi Med Univ，2023，40（5）：733-741.

LIU X，PAN Y C，SHEN Y M，et al. Protective effects of Abrus cantoniensis Hance on the fatty liver hemorrhagic syndrome in laying hens based on liver metabolomics and gut microbiota[J]. Front Vet Sci，2022，9：862006.

付振贺，黄玉凤，黄超君，等. HPLC-TOF/MS快速鉴别相思藤中的化学成分[J]. 第二军医大学学报，2018，39（3）：329-333.

FU Z H，HUANG Y F，HUANG C J，et al. Rapid identification of chemical constituents in Yine Abrus （Xiangsiteng） by HPLC-TOF/MS[J]. Acad J Second Mil Med Univ，2018，39（3）：329-333.

郭忠会，郑雪莹，梁洁，等. UPLC-Q-TOF/MS技术结合UNIFI平台快速鉴定鸡骨草化学成分[J]. 中国药房，2022，33（23）：2852-2857，2863.

GUO Z H，ZHENG X Y，LIANG J，et al. Rapid identification of chemical constituents in Abrus cantoniensis Hance by UPLC-Q-TOF/MS combined with UNIFI platform[J]. China Pharm，2022，33（23）：2852-2857，2863.

周子力. 广金钱草中三种黄酮碳苷的制备与含量测定[D]. 成都：西南交通大学，2013.

ZHOU Z L. Preparation and content determination of three flavonoids in Desmodium Styracifolium[D]. Chengdu：Southwest Jiaotong University，2013.

刘美静，张郭慧，黄小桃，等. 夏佛塔苷对高脂饮食诱导小鼠非酒精性脂肪肝的保护作用[J]. 中华中医药杂志，2017，32（11）：5078-5081.

LIU M J，ZHANG G H，HUANG X T，et al. Protection effect of schaftoside on mice with non-alcoholic fatty liver induced by high fat diet[J]. China J Tradit Chin Med Pharm，2017，32（11）：5078-5081.

DANG J，PAUDEL Y N，YANG X L，et al. Schaftoside suppresses pentylenetetrazol-induced seizures in zebrafish via suppressing apoptosis，modulating inflammation，and oxidative stress[J]. ACS Chem Neurosci，2021，12（13）：2542-2552.

YANG M，ZAHARNA M A，CHEN Y S，et al. In vitro antioxidant activities and anti-proliferative properties of the functional herb Abrus cantoniensis and its main alkaloid abrine[J]. Food Funct，2014，5（9）：2268-2277.

陈学芬，钟正贤，李燕婧，等. 海帕刺桐碱药理作用的研究[J]. 中国中医药科技，2009，16（5）：372-373.

CHEN X F，ZHONG Z X，LI Y J，et al. Study on pharmacological action of erythrine[J]. Chin J Tradit Med Sci Technol，2009，16（5）：372-373.

李太平，吴芳芳，孙晖，等. 基于网络药理学和分子对接探究壮药鸡骨草治疗乙型肝炎的作用机制[J]. 现代药物与临床，2022，37（1）：33-41.

LI T P，WU F F，SUN H，et al. Exploring the mechanism of the Abrus cantoniensis in the treatment of hepatitis B based on network pharmacology and molecular docking[J]. Drugs Clin，2022，37（1）：33-41.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

Address：8th Floor, Sihuan Building, No. 129 Daping Main Street, Yuzhong District, Chongqing, China Postal code：400042
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Analysis of in vivo components and metabolism pathway in rats after intragastric administration of aqueous extract from Abrus cantoniensis

DOI：10.6039/j.issn.1001-0408.2024.13.06

摘要

Abstract

关键词

Keywords

references